1. Stem Cell Research Center, Department of Regenerative Medicine, Tongji University School of Medicine, Tongji University, Shanghai 200092, China; 2. Advanced Institute of Translational Medicine, Tongji University, Shanghai 200092, China; 3. School of Life Sciences and Technology, Tongji University, Shanghai 200092, China; 4. Department of Human Genetics, UCLA, Los Angeles, CA 90095, USA
The newly developed next-generation sequencing platforms, in combination with genome-scale amplification methods, provide a powerful tool to study genomics from a single cell. This mini-review summarizes the technologies of single cell genomics and their applications in several areas of biomedical research including stem cells, cancer biology and reproductive medicine. Particularly, it highlights recent advances in single cell exome sequencing, RNA-seq, and genome sequencing. The application of these powerful techniques will shed new light on the fundamental principles of gene transcription and genome organization at single-cell level and improve our understanding of cellular heterogeneity and diversity in multicellular organisms.
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