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Cloning and characterization of PhPI9 involved in floral development from Phalaenopsis Orchid |
| GUO Bin, DAI Wei, CHEN Donghong, WEI Xing, MING Feng |
| Institute of Genetics, State Key Laboratory of Genetic Engineering, Research Center of Gene Diversity and Designed Agriculture, Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life Science, Fudan University, Shanghai 200433, China |
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Abstract In the attempt to discover new genes involved in the floral development in monocotyledonousin species, we have cloned and characterized the homologous PISTALLATA-like (PI-like) gene from Phalaenopsis hybrid cultivar named PhPI9 (Phalaenopsis PI STILLATA # 9). The cDNA of PhPI9 has a fragment of 834 bp and has 60% identity with the PISTILATA from Arabidopsis. The deduced amino acid sequence of PhPI9 had the typical PI-motif. It also formed a subclade with other monocot PI-type genes in phylogenetic analysis. Southern analysis showed that PhPI9 was present in the Phalaenopsis orchid genome as a single copy. Furthermore, it was expressed only in the lip of the Phalaenopsis flower and no expression was detected in vegetative organs. Thus, as a B-function MADS-box gene, PhPI9 specifies floral organ identity in orchids.
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Issue Date: 05 December 2007
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