|
|
|
Construction of a recombinant yeast strain converting
xylose and glucose to ethanol |
| TIAN Shen1, ZANG Jinxin1, PAN Yaping1, LIU Jikai1, YANG Xiushan1, YUAN Zhenhong2, YAN Yongjie2 |
| 1.College of Life Science, Capital Normal University; 2.East China University of Science and Technology |
|
|
|
|
Abstract Candida shehatae gene xyl1 and Pichia stipitis gene xyl2, encoding xylose reductase (XR) and xylitol dehydrogenase (XD) respectively, were amplified by PCR. The genes xyl1 and xyl2 were placed under the control of promoter GAL in vector pYES2 to construct the recombinant expression vector pYES2-P12. Subsequently the vector pYES2-P12 was transformed into S. cerevisiae YS58 by LiAc to produce the recombinant yeast YS58-12. The alcoholic ferment indicated that the recombinant yeast YS58-12 could convert xylose to ethanol with the xylose consumption rate of 81.3%.
|
|
Issue Date: 05 June 2008
|
|
| 1 |
Aristos A Merja P 2000 Metabolicengineering applications to renewable resource utilizationCurrent Opinion in Biotechnology11187198.
doi:10.1016/S0958‐1669(00)00085‐9
|
| 2 |
Bruce S D 2000 Development of new ethanologenic Escherichia coli strains for fermentation of lignocellulosicbiomassApplied Biochemistry and Biotechnology84181196.
doi:10.1385/ABAB:84‐86:1‐9:181
|
| 3 |
Gietz R D Schiestl R H Willems A R Woods R A 1995 Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEGprocedureYeast11(4)355360.
doi:10.1002/yea.320110408
|
| 4 |
Gong C S Cao N J Du J 1999 Ethanol production from renewable resourseAdv Biochem Eng Biotechnol65207241
|
| 5 |
Hallborn J Walfridsson M Airaksinen U Ojamo H Hahn-Hägerdal B Penttila M Keränen S 1991 Xylitol production by recombinant Saccharomyces cerevisiaeBioTechnology910901095
|
| 6 |
Kotter P Amore R Hollenberg C P Ciriacy M 1990 Isolation and characterization of the Pichiastipitis xylitol dehydrogenase gene, xyl2, and construction of a xylose-utilizing Saccharomyces cerevisiae transformantCurr Genet18493.
doi: 10.1007/BF00327019
|
| 7 |
Sambrook J David W R 2001 Molecularcloning: A Laboratory Manual, 3rd edNew YorkCold Spring Harbor LaboratoryPress
|
| 8 |
Xie L P Wang Z X Zhu G J 2001 Application of metabolic engineering ofbacteria and yest in ethanol production from renewable resourceFood and Fermentation Industry27(12)6368 (in Chinese)
|
| 9 |
Zaldivar J Nielsen J Olsson L 2001 Fuel ethanol production from lignocellulose:a challenge for metabolic engineering and process integrationAppl Microbiol Biotechnol561734.
doi:10.1007/s002530100624
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
