|
|
|
Molecular phylogenetic relationship of based on sequences of mtDNA |
| ZHU Shihua, YANG Yingchun, ZHENG Wenjuan, SHEN Xiquan, ZOU Jixing |
| Faculty of Life Science and Biotechnology, Ningbo University |
|
|
|
|
Abstract The mtDNA Cyt b gene was sequenced partially for Variola louti of Serranidae, Epinephelinae and seven endemic species of groupers-Epinephelus awoara, E. brunneus, E. coioides, E. longispinis, E. sexfasciatus, E. spilotoceps and E. tauvina in China. The seven endemic species and other seven foreign species of groupers-E. aeneus, E. caninus, E. drummondhayi, E. haifensis, E. labriformis, E. marginatus and E. multinotatus from the GenBank were combined and analysed as ingroup, while Variola louti was used as outgroup. We compared the 420 bp sequences of Cyt b among the 15 species and constructed two types of molecular phylogenetic trees with maximum parsimony method (MP) and neighbor-joining method (NJ) respectively. The results were as follows: (1) As to the base composition of mtDNA Cyt b sequence (402 bp) of 14 species of Epinephelus, the content of (A + T) was 53.6%, higher than that of (G + C) (46.4%). The transition/transversion ratio was 4.78 with no mutation saturation. (2) The cluster relationships between E. awoara and E. sexfasciatus, E. coioides and E. tauvina, E. longispinis and E. spilotoceps were consistent with phenotypes in taxonomy. (3) In the phylogenetic tree, the species in the Atlantic Ocean were associated closely with those in the Pacific Ocean, which suggested that the Cyt b sequences of Epinephelus were highly conserved. This may be attributed to the coordinate evolution. (4) In wel1-bred mating or heredity management, mating Epinephelus of the same branch should be avoided. It is likely to be an effective way to mate the species of the Atlantic Ocean with those of the Pacific Ocean to improve the inheritance species.
|
|
Issue Date: 05 June 2008
|
|
| 1 |
Cantatore P Roberti M Pesole G Ludovico A Milella F Gadaleta M N Saccone C 1994 Evolutionaryanalysis of cytochrome b sequencesin some Perciformes evidence for a slower rate of evolution than inmammalsJ. Mol. Evol.39589597.
doi:10.1007/BF00160404
|
| 2 |
Chen Q C Chen Z 2002 Fishes fromNansha Islands to South China Ccoastal Wwaters II (M)BeijingScience Press105106 (in Chinese)
|
| 3 |
Cheng Q T Zheng B S 1987 Systematicsynopsis of Chinese FfishesBeijingScience Press289296 (in Chinese)
|
| 4 |
Felsenstein J 1985 Confidence limits on phylogenies: anapproach using thebootstrapEvolution39783791.
doi:10.2307/2408678
|
| 5 |
Gilles A Miquelis A Quignard J P Faure E 2000 Molecular phylogeography of western Mediterranean dusky grouper Epinephelus marginatusLife Sci323(2)195205
|
| 6 |
Hassanin A Lecointer G Tillier S 1998 The “ Evolutionary Signal ”of homoplasy in Pprotein coding gene sequences and its consequencesfor a priori weighting in phylogenyComptesRendus de l'Académie des Sciences321611620
|
| 7 |
He S P Liu H Z Chen Y Y Kuwahara M Nakajima T Zhong Y 2004 Molecular phylogenetic relationships of Eastern Asian Cyprinidae(Pisces: Cypriniformes) inferred from cytochrome b sequencesScience in ChinaSeries C-Life Sciences47(2)130138.
doi:10.1360/03yc0034
|
| 8 |
Heemstra P C Randall J E 1993 Groupers ofthe WworldRomeFAO Fisheries Synopsis 125184186
|
| 9 |
Huang Z G 1994 Marine species and their distributions in China's SeasBeijingChinaOcean Press693695 (in Chinese)
|
| 10 |
Kumar S Tamura K Nei M 2004 MEGA3: Integrated software for MolecularEvolutionary Genetics Analysis and sequence alignmentBriefings in Bioinformatics5150163.
doi:10.1093/bib/5.2.150
|
| 11 |
Maggio T Andaloro F Hemida F Arculeo M 2005 A molecular analysis of some Eastern Atlantic grouper from the Epinephelus and Mycteroperca genusJournal of Experimental Marine Biologyand Ecology321(1)8392.
doi:10.1016/j.jembe.2005.01.004
|
| 12 |
Peng Z G He S P Zhang Y G 2002 Mitochondrial cytochrome b sequence variationand phylogeny of the East Asian bagrid catfishesProg. Nat . Sci.12(6)596600
|
| 13 |
Shen S C 1986 Synopsis of Fishes of TaiwanSouthernMaterials Center, Inc.Taipei, Republicof China262269 (in Chinese).
|
| 14 |
Tang Q Y Yang X P Liu H Z 2003 Biogeographical process of spinibarbuscaldweli revealed by sequence variations of mitochondrial cytochrome b geneActa HydrobiologicalSinica27(4)352356 (in Chinese)
|
| 15 |
Wu H L Shao K T Lai C F 1999 Lantin-Chinese dictionary of fishes namesTaipeiFisheryPress320324 (in Chinese)
|
| 16 |
Xiang F Zou J X Deng F J Liu S Y Sui Y 2004 The molecular taxonomy and phylogeny ofzebrafish (Danio rerio) Bbasedon the Cytb Ppartial SsequencesChinese Journal of Zoology39(5)1318 (in Chinese)
|
| 17 |
Zou J X Hu C Q Xiao Y X 2000 Studies on the techniques of sex controland rearing in EpinephelusReservoir Fisheries20(2)13 (in Chinese)
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
