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Frontiers in Biology

ISSN 1674-7984

ISSN 1674-7992(Online)

CN 11-5892/Q

Front. Biol.    2008, Vol. 3 Issue (2) : 203-206    https://doi.org/10.1007/s11515-008-0030-1
Induction, selection and antibacterial activity of the antibacterial peptides from lepidopteran insect cultured cell lines
PENG Rong1, LIU Kaiyu1, YAO Hanchao1, YANG Hong1, HONG Huazhu1, YANG Zhong2, CUI Yanfang3
1.College of Life Science, Central China Normal University; 2.College of Bioengineering, East China University of Science and Technology; 3.College of Chemistry, Central China Normal University
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Abstract We induced 3 cell lines that were in vitro cultured from Lepidoptera with heat inactivated Escherichia coli DH5? to stimulate the antibacterial peptide followed by antibacterial activity assay, induction dynamic research and Tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Tricine SDS-PAGE) experiment. The antibacterial activity of the induced BTI-Tn-5B1 cell line was the highest, and the antibacterial activity increased gradually to the highest level in 16 hours after stimulation. A new antibacterial peptide with a molecular weight of about 8000 Da was preferentially induced in Trichoplusia ni BTI-Tn-5B1 cells in 16 hours after stimulation. Antibacterial activity assays indicated that it had inhibition against Staphylococcus aureus, Escherichia coli K12D31 and Salmonella derby. It has especially strong inhibition against Gram-negative bacteria such as Escherichia coli K12D31 and Salmonella derby.
Issue Date: 05 June 2008
 Cite this article:   
PENG Rong,YANG Zhong,YANG Hong, et al. Induction, selection and antibacterial activity of the antibacterial peptides from lepidopteran insect cultured cell lines[J]. Front. Biol., 2008, 3(2): 203-206.
 URL:  
https://academic.hep.com.cn/fib/EN/10.1007/s11515-008-0030-1
https://academic.hep.com.cn/fib/EN/Y2008/V3/I2/203
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