Cloning and expression of endo-β-glucanase
II gene in  H31-3

doi:10.1007/s11515-008-0053-7

Front. Biol.

2008, Vol. 3

Issue (3) : 287-292 https://doi.org/10.1007/s11515-008-0053-7

Cloning and expression of endo-β-glucanase II gene in H31-3

TAN Huifang¹, ZHANG Guoqing², CUI Fumian², QIAN Shijun², ZHENG Guangyu³

1.Institute of Microbiology, Chinese Academy of Sciences; Beijing Normal University; 2.Institute of Microbiology, Chinese Academy of Sciences; 3.Beijing Normal University;

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Abstract Endo-?-glucanase II (EG II) gene cDNA was isolated from the fungus Humicola insolens H31-3 by RT-PCR. It was cloned into the expression vector pGAPZ?A. The resultant recombinant plasmid was introduced into Pichia pastoris GS115 by electroporation after being linearized by BspHI digestion. The recombinant Pichia pastoris strain was obtained and SDS-PAGE showed that the molecular weight of the expression protein was about 55 kD.The cultivation condition and the characteristics of the recombinant EG II were also explored.

Issue Date: 05 September 2008

Cite this article:

TAN Huifang,ZHANG Guoqing,QIAN Shijun, et al. Cloning and expression of endo-β-glucanase II gene in H31-3[J]. Front. Biol., 2008, 3(3): 287-292.

URL:

https://academic.hep.com.cn/fib/EN/10.1007/s11515-008-0053-7
https://academic.hep.com.cn/fib/EN/Y2008/V3/I3/287

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