Effects of plant growth regulators on the rapid proliferation of shoots and root induction in the Chinese traditional medicinal plant <italic>Atractylodes macrocephala

doi:10.1007/s11515-009-0006-9

Front Biol Chin

2009, Vol. 4

Issue (2) : 217-221 https://doi.org/10.1007/s11515-009-0006-9

RESEARCH ARTICLE

Effects of plant growth regulators on the rapid proliferation of shoots and root induction in the Chinese traditional medicinal plant Atractylodes macrocephala

Bizeng MAO¹(

), Bowei HE², Zaiming CHEN¹, Bingliang WANG¹, Huifeng PAN², Debao LI¹

1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; 2. Agricultural Department of Zhejiang Province, Hangzhou 310020, China

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Abstract

We developed an efficient plant regeneration protocol for rapidly propagating Atractylodes macrocephala Koidz, an important traditional Chinese medicinal plant, via shoot organogenesis. Shoot multiplication was induced on Murashige-Skoog (MS) medium supplemented with various concentrations of N-phenyl-N-1,2,3-thidiazol-5-ylurea (TDZ), 6-benzylaminopurine (BA) and α-naphthaleneacetic acid (NAA). Rooting was induced on half-strength MS medium supplemented with NAA and indolebutyric acid (IBA). The maximum mean number of shoots (5.61) was obtained from a single explant by the combined effect of 1.08 μmol/L NAA and 2.25 μmol/L TDZ. The longest roots and a minimum number of roots were produced when they were cultured in a medium without plant growth regulators. The shortest roots and the largest number of roots were observed in the medium supplemented with 2.7 μmol/L NAA.

Keywords Chinese traditional medicinal plant Atractylodes macrocephala Koidz multiple shoot plant regeneration

Corresponding Author(s): MAO Bizeng,Email:maobz@zju.edu.cn

Issue Date: 05 June 2009

Cite this article:

Bizeng MAO,Bowei HE,Zaiming CHEN, et al. Effects of plant growth regulators on the rapid proliferation of shoots and root induction in the Chinese traditional medicinal plant Atractylodes macrocephala[J]. Front Biol Chin, 2009, 4(2): 217-221.

URL:

https://academic.hep.com.cn/fib/EN/10.1007/s11515-009-0006-9
https://academic.hep.com.cn/fib/EN/Y2009/V4/I2/217

Fig.1 Variations of growth parameters in response to different sterilization treatments. (1) Sterilized with 0.1% (w/v) aqueous mercuric chloride for six min; (2) Sterilized with 0.1% (w/v) aqueous mercuric chloride for eight min; (3) Sterilized with 0.1% (w/v) aqueous mercuric chloride for 10 min; (4) Sterilized with 10% aqueous solution of 5.4% sodium hypochlorite for 10 min; (5) Sterilized with 10% aqueous solution of 5.4% sodium hypochlorite for 15 min. Bars with different letters are significantly different (≤0.05).

Tab.1 Effect of PGRs on shoot regeneration of Baizhu

Fig.2 Development of a regeneration system for Baizhu through shoot multiplication. A: Proliferation of regenerated shoots (bar=1 cm); B: Cluster of shoots proliferated from bud explant on MS medium containing 2.25 μmol/L TDZ and 1.08 μmol/L NAA after 4 weeks in culture (bar=1 cm); C: Plantlet with regenerated root system before transfer to soil (bar=1 cm); D: Plant in the greenhouse 2 months after transplantation (bar=2 cm); E: Mature normal plants produced in the greenhouse (bar=4 cm); F: Flower bud of the regenerated plant (bar=1 cm); G: Mature flower of regenerated plant (bar=1 cm).

Tab.2 Effect of PGRs on root formation from shoots

Fig.3 Effects of plant growth regulators on root formation after four weeks of culture. Rooting of regenerated shoots in half-strength MS medium, A: without plant growth regulators; B: containing 2.45 μmol/L IBA; C: containing 1.08 μmol/L NAA; D: containing 2.7 μmol/L NAA. Bars=1 cm.

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