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Inhibition of vascular endothelial growth factor
expression by Chinese medicine of Hedyotis
diffusa Willd herbal compounds |
| Min WANG1,Zhumei SHI1,Jiahao SHA1,Dan LIU2,Gong-Yu ZHANG3,Bing-Hua JIANG4, |
| 1.Laboratory of Reproductive
Medicine, Department of Pathology, Cancer Center, Nanjing Medical
University, Nanjing 210029, China; 2.Laboratory of Reproductive
Medicine, Department of Pathology, Cancer Center, Nanjing Medical
University, Nanjing 210029, China；School of Life Science,
Soochow University, Suzhou 215021, China; 3.Nanjing University of
Traditional Chinese Medicine, Nanjing 210046, China; 4.Laboratory of Reproductive
Medicine, Department of Pathology, Cancer Center, Nanjing Medical
University, Nanjing 210029, China；Department of Pathology,
Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia,
PA 19107, USA; |
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Abstract The Hedyotis diffusa Willd herbal compounds (HDWHCs) are commonly used as Chinese medicine to treat cancer patients with established clinical therapeutic efficacy in China. However, the underlying mechanisms remain to be elucidated. In this study, we used freeze-dried powder of the water extracts of HDWHCs to investigate the potential mechanisms of HDWHCs in cancer treatment. HDWHCs treatment significantly inhibited vascular endothelial growth factor (VEGF) mRNA levels and VEGF transcriptional activation in cancer cells. HDWHCs also had a remarkable inhibitory effect on the expression of hypoxia-inducible factor 1alpha (HIF-1alpha). Forced expression of HIF-1α restored VEGF transcriptional activation inhibited by HDWHCs, indicating that HDWHCs suppressed VEGF expression through decreasing HIF-1alpha expression. Moreover, HDWHCs inhibited cyclooxygenase-2 (COX-2) expression, and overexpression of HIF-1alpha restored HDWHCs’ inhibitory effect on COX-2 at transcriptional level. These findings may provide better understanding of HDWHCs’ anti-cancer mechanism in cancer treatment.
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| Keywords
Hedyotis diffusa Willd
Chinese medicine
vascular endothelial growth factor (VEGF)
hypoxia-inducible factor 1alpha (HIF-1alpha)
cyclooxygenase-2 (COX-2)
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Issue Date: 01 August 2010
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