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New PCR based markers allowed to identify Secale chromatin in wheat- Secale africanum introgression lines |
| Juqing JIA,Guangrong LI,Cheng LIU,Jianping ZHOU,Zujun YANG, |
| School of Life Science
and Technology, University of Electronic Science and Technology of
China, Chengdu 610054, China; |
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Abstract The genus of Secale has many agronomically important characters. In order to use the best of this species, markers tracking the rye chromatin incorporated into wheat must be developed. In this study, one rye genome-specific random amplified polymorphic DNA (RAPD) marker was isolated from Secale africanum (Ra genome). Two cloned markers, named OPP131165 and OPP13662, were 1165 bp and 662 bp, respectively. Sequence analysis revealed that OPP131165 was highly homologous to a part of a new class of transposon-like gene called the Revolver family, and OPP13662 was partially similar to LTR gypsy-like retrotransposon. Fluorescence in situ hybridization (FISH) showed only OPP131165 localized within the whole arms of rye except their terminal regions and no signal was detected on wheat chromosomes, while OPP13662 had no hybridization signal detected on wheat and rye genomes. Based on these sequences, two pairs of sequence-characterized amplified region (SCAR) primers were designed, and the resulted SCAR markers were able to target both cultivated and wild Secale species. The FISH patterns and the two SCAR markers should be able to identify and track all wheat-rye translocation lines, especially the S. africanum chromatin.
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| Keywords
Secale africanum
rye
molecular marker
fluorescence in situ hybridization (FISH)
chromatin detection
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Issue Date: 01 April 2010
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Alkhimova A G, Heslop-Harrison J S, Shchapova A I, Vershinin A V (1999). Rye chromosome variability in wheat-rye addition and substitution lines. Chromosome Res, 7: 205–212
doi: 10.1023/A:1009299300018
|
|
Brunell M S, Lukaszewski A J, Whitkus R (1999). Development of arm specific RAPD markers for rye chromosome 2R in wheat. Crop Sci, 39: 1702–1706
|
|
Francki M G (2001). Identification of Bilby, a diverged centromeric Ty1-copia retrotransposonfamily from cereal rye (Secale cereale L.). Genome, 44: 266–274
doi: 10.1139/gen-44-2-266
|
|
Iqbal M J, Rayburn A L (1995). Identification of the 1RS rye chromosomal segment inwheat by RAPD analysis. Theor Appl Genet, 91: 1048–1053
doi: 10.1007/BF00223918
|
|
Ito H, Nasuda S, Endo T R (2004). A direct repeat sequence associated with the centromeric retrotransposons inwheat. Genome, 47: 747–756
doi: 10.1139/g04-034
|
|
Jia J Q, Yang Z J, Li G R, Liu C, Lei M P, Zhang T, Zhou J P, Ren Z L (2009). Isolation and chromosomal distribution of a novel Ty1-copia-like sequence from Secale, which enables identification of wheat-Secale africanum introgression lines. J Appl Genet, 50(1): 25–28
|
|
Jiang H R, Dai D Q, Sun D F (1992). Creation of special germaplasm resources in Triticum. Journal of Sichuan Agricultural University, 10(2): 255–259
|
|
Ko J M, Do G S, Suh D.Y, Seo B B, Shin D C, Moon H P (2002). Identification and chromosomal organization of two rye genome-specific RAPD products useful as introgression markersin wheat. Genome, 45: 157–164
doi: 10.1139/g01-133
|
|
Lapitan N L V, Sears R G, Gill B S (1988). Amplification of repeated DNA sequences in wheat -rye hybrids regenerated from tissueculture. Theor Appl Genet, 75: 381–388
doi: 10.1007/BF00276739
|
|
Linares C, Serna A, Fominaya A (1999). Chromosomal organization of a sequence related to LTR-like elements of Ty1-copia retrotransposons in Avena species. Genome, 42: 706–713
doi: 10.1139/gen-42-4-706
|
|
Liu C, Yang Z J, Li G R, Zeng Z X, Zhang T, Zhou J P, Liu Z H, Ren Z L (2008). Isolation of a new repetitive DNA sequence from Secale africanum enables targeting of Secale chromatin in wheat background. Euphytica, 159: 249–258
doi: 10.1007/s10681-007-9484-5
|
|
McIntyre C L, Pereira S, Moran L B, Appels R (1990). New Secale cereale (rye) DNA derivatives for the detection of ryechromosome segments in wheat. Genome, 33: 635–640
|
|
Mukai Y, Friebe B, Hatchett J H, Yamamoto M, Gill B S (1993). Molecular cytogenetic analysis of radiation-induced wheat-rye terminaland intercalary chromosomal translocations and the detection of ryechromatin resistance to Hessian fly. Chromosoma, 102: 88–95
doi: 10.1007/BF00356025
|
|
Nkongolo K K, Lapitan N L V, Quick J S, Muhlmann M D (1993). An optimized fluorescence in situ hybridization procedure for detecting rye chromosomes in wheat. Genome, 36: 701–705
doi: 10.1139/g93-094
|
|
Singh R J, Robbelen G (1977). Identification by Giemsa technique of the translocationsseparating cultivated rye from three wild species of Secale. Chromosoma, 59(3): 217–225
doi: 10.1007/BF00292779
|
|
Tomita M, Shinohara K, Morimoto M (2008). Revolver is a new class of transposon-likegene composing the Triticeae genome. DNA Research, 15: 49–62
doi: 10.1093/dnares/dsm029
|
|
Yang Z J, Li G R, Feng J, Jiang H R, Ren Z L (2005). Molecular cytogenetic characterization and disease resistance observation ofwheat-Dasypyrum breviaristatum partial amphiploid and its derivatives. Hereditas, 142: 80–85
doi: 10.1111/j.1601-5223.2005.01918.x
|
|
Yang Z J, Li G R, Ren Z L (2001). Identification of Triticum durum-Secale africanum amphiploid and its crossability with common wheat. J Genet Breed, 55: 45–50
|
|
Zeller F J, Hsam S L K (1984). Broadening the genetic variability of cultivated wheatby utilizing rye chromatin. In: Sakamoto S, ed. Proc Intl Wheat Genet Symp,6th, 161–173
|
|
Zhang P, Li W, Fellers J, Friebe B, Gill B S (2004). BAC-FISH in wheat identifies chromosome landmarks consisting of different typesof transposable elements. Chromosoma, 112: 288–299
doi: 10.1007/s00412-004-0273-9
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