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Techniques of cell type-specific transcriptome analysis and applications in researches of sexual plant reproduction |
Tian-Xiang HU, Miao YU, Jie ZHAO( ) |
| Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China |
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Abstract In higher plants, specific cell differentiation and fate decision are controlled by differential gene expression. Cell type-specific transcriptome analysis has become an important tool for investigating cell regulatory mechanisms. In recent years, many different techniques have been developed for the isolation of specific cells and the subsequent transcriptome analysis, and considerable data are available regarding the transcriptional profiles of some specific cells. These cell type-specific transcriptome analyses hold significant promise for elucidating the gene expression linked to cellular identities and functions, and are extraordinarily important for research in functional genomics and systems biology aimed toward basic understanding of molecular networks and pathway interactions. Moreover, to reveal the critical mechanisms about sexual plant reproduction, the gamete and embryo cells have long been treated as good subjects for cell-specific transcriptome analysis, and there has been important progress in recent decades. In this review, we summarize current technologies in cell type-specific transcriptome analysis and review the applications of these technologies in research into the mechanisms of sexual reproduction in higher plants.
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| Keywords
specific cell
transcriptome
plant reproduction
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Corresponding Author(s):
ZHAO Jie,Email:jzhao@whu.edu.cn
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Issue Date: 01 February 2011
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|
| 1 |
Aharoni A, Vorst O (2002). DNA microarrays for functional plant genomics. Plant Mol Biol , 48(1-2): 99-118
doi: 10.1023/A:1013734019946 pmid:11860216
|
| 2 |
Ansorge W J (2009). Next-generation DNA sequencing techniques. New Biotechnol , 25(4): 195-203
doi: 10.1016/j.nbt.2008.12.009 pmid:19429539
|
| 3 |
Asano T, Masumura T, Kusano H, Kikuchi S, Kurita A, Shimada H, Kadowaki K (2002). Construction of a specialized cDNA library from plant cells isolated by laser capture microdissection: toward comprehensive analysis of the genes expressed in the rice phloem. Plant J , 32(3): 401-408
doi: 10.1046/j.1365-313X.2002.01423.x pmid:12410817
|
| 4 |
Baginsky S, Gruissem W (2006). Arabidopsis thaliana proteomics: from proteome to genome. J Exp Bot , 57(7): 1485-1491
doi: 10.1093/jxb/erj130 pmid:16551684
|
| 5 |
Becker J D, Boavida L C, Carneiro J, Haury M, Feijó J A (2003). Transcriptional profiling of Arabidopsis tissues reveals the unique characteristics of the pollen transcriptome. Plant Physiol , 133(2): 713-725
doi: 10.1104/pp.103.028241 pmid:14500793
|
| 6 |
Belyavsky A, Vinogradova T, Rajewsky K (1989). PCR-based cDNA library construction: general cDNA libraries at the level of a few cells. Nucleic Acids Res , 17(8): 2919-2932
doi: 10.1093/nar/17.8.2919 pmid:2471144
|
| 7 |
Birnbaum K, Shasha D E, Wang J Y, Jung J W, Lambert G M, Galbraith D W, Benfey P N (2003). A gene expression map of the Arabidopsis root. Science , 302(5652): 1956-1960
doi: 10.1126/science.1090022 pmid:14671301
|
| 8 |
Borges F, Gomes G, Gardner R, Moreno N, McCormick S, Feijó J A, Becker J D (2008). Comparative transcriptomics of Arabidopsis sperm cells. Plant Physiol , 148(2): 1168-1181
doi: 10.1104/pp.108.125229 pmid:18667720
|
| 9 |
Brady S M, Orlando D A, Lee J Y, Wang J Y, Koch J, Dinneny J R, Mace D, Ohler U, Benfey P N (2007). A high-resolution root spatiotemporal map reveals dominant expression patterns. Science , 318(5851): 801-806
doi: 10.1126/science.1146265 pmid:17975066
|
| 10 |
Brandt S, Kehr J, Walz C, Imlau A, Willmitzer L, Fisahn J (1999). Technical advance: A rapid method for detection of plant gene transcripts from single epidermal, mesophyll and companion cells of intact leaves. Plant J , 20(2): 245-250
doi: 10.1046/j.1365-313x.1999.00583.x pmid:10571884
|
| 11 |
Brandt S, Kloska S, Altmann T, Kehr J (2002). Using array hybridization to monitor gene expression at the single cell level. J Exp Bot , 53(379): 2315-2323
doi: 10.1093/jxb/erf093 pmid:12432024
|
| 12 |
Cao Y, Reece A, Russell S D (1996). Isolation of viable sperm cells from tobacco (Nicotiana tabacum). Zygote , 4(2): 81-84
doi: 10.1017/S096719940000294X pmid:8913020
|
| 13 |
Casson S, Spencer M, Walker K, Lindsey K (2005). Laser capture microdissection for the analysis of gene expression during embryogenesis of Arabidopsis. Plant J , 42(1): 111-123
doi: 10.1111/j.1365-313X.2005.02355.x pmid:15773857
|
| 14 |
Chen S H, Yang Y H, Liao J P, Kuang A X, Tian H Q (2008). Isolation of egg cells and zygotes of Torenia fournieri L. and determination of their surface charge. Zygote , 16(2): 179-186
doi: 10.1017/S0967199408004693 pmid:18405439
|
| 15 |
Diatchenko L, Lau Y F, Campbell A P, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov E D, Siebert P D (1996). Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc Natl Acad Sci USA , 93(12): 6025-6030
doi: 10.1073/pnas.93.12.6025 pmid:8650213
|
| 16 |
Dresselhaus T, L?rz H, Kranz E (1994). Representative cDNA libraries from few plant cells. Plant J , 5(4): 605-610
doi: 10.1046/j.1365-313X.1994.5040605.x pmid:8012409
|
| 17 |
Elling A A, Deng X W (2009). Next-generation sequencing reveals complex relationships between the epigenome and transcriptome in maize. Plant Signal Behav , 4(8): 760-762
doi: 10.4161/psb.4.8.9174 pmid:19820310
|
| 18 |
Emmert-Buck M R, Bonner R F, Smith P D, Chuaqui R F, Zhuang Z, Goldstein S R, Weiss R A, Liotta L A (1996). Laser capture microdissection. Science , 274(5289): 998-1001
doi: 10.1126/science.274.5289.998 pmid:8875945
|
| 19 |
Engel M L, Chaboud A, Dumas C, McCormick S (2003). Sperm cells of Zea mays have a complex complement of mRNAs. Plant J , 34(5): 697-707
doi: 10.1046/j.1365-313X.2003.01761.x pmid:12787250
|
| 20 |
Fu C M, Sun M X, Zhou C, Yang H Y (1996). Isolation of fertilized embryo sacs and zygotes and triggering of zygote division in vitro in Nicotiana tabacum. Acta Bot Sin , 38: 262-267
|
| 21 |
Ge X, Chen W, Song S, Wang W, Hu S, Yu J (2008). Transcriptomic profiling of mature embryo from an elite super-hybrid rice LYP9 and its parental lines. BMC Plant Biol , 8(1): 114
doi: 10.1186/1471-2229-8-114 pmid:19000321
|
| 22 |
Givan A L (2001). Flow Cytometry First Principles.New York: Wiley-Liss,. 273
|
| 23 |
Goldberg R B, Hoschek G, Ditta G S, Breidenbach R W (1981). Developmental regulation of cloned superabundant embryo mRNAs in soybean. Dev Biol , 83(2): 218-231
doi: 10.1016/0012-1606(81)90468-1 pmid:6113180
|
| 24 |
Gou X, Yuan T, Wei X, Russell S D (2009). Gene expression in the dimorphic sperm cells of Plumbago zeylanica: transcript profiling, diversity, and relationship to cell type. Plant J , 60(1): 33-47
doi: 10.1111/j.1365-313X.2009.03934.x pmid:19500307
|
| 25 |
Gou X P, Xu Y, Tang L, Yan F, Chen F (2001). Representative cDNA library from isolated rice sperm cells. Acta Bot Sin , 43: 1093-1096
|
| 26 |
Haerizadeh F, Wong C E, Bhalla P L, Gresshoff P M, Singh M B (2009). Genomic expression profiling of mature soybean (Glycine max) pollen. BMC Plant Biol , 9(1): 25
doi: 10.1186/1471-2229-9-25 pmid:19265555
|
| 27 |
Herzenberg L A, De Rosa S C (2000). Monoclonal antibodies and the FACS: complementary tools for immunobiology and medicine. Immunol Today , 21(8): 383-390
doi: 10.1016/S0167-5699(00)01678-9 pmid:10916141
|
| 28 |
Hobo T, Suwabe K, Aya K, Suzuki G, Yano K, Ishimizu T, Fujita M, Kikuchi S, Hamada K, Miyano M, Fujioka T, Kaneko F, Kazama T, Mizuta Y, Takahashi H, Shiono K, Nakazono M, Tsutsumi N, Nagamura Y, Kurata N, Watanabe M, Matsuoka M (2008). Various spatiotemporal expression profiles of anther-expressed genes in rice. Plant Cell Physiol , 49(10): 1417-1428
doi: 10.1093/pcp/pcn128 pmid:18776202
|
| 29 |
Holm P B, Knudsen S, Mouritzen P, Negri D, Olsen F L, Roue C (1994). Regeneration of fertile barley plants from mechanically isolated protoplasts of the fertilized egg cell. Plant Cell , 6(4): 531-543
doi: 10.1105/tpc.6.4.531 pmid:12244247
|
| 30 |
Honys D, Twell D (2003). Comparative analysis of the Arabidopsis pollen transcriptome. Plant Physiol , 132(2): 640-652
doi: 10.1104/pp.103.020925 pmid:12805594
|
| 31 |
Honys D, Twell D (2004). Transcriptome analysis of haploid male gametophyte development in Arabidopsis. Genome Biol , 5(11): R85
doi: 10.1186/gb-2004-5-11-r85 pmid:15535861
|
| 32 |
Hu S Y, Li L G, Zhu C (1985). Isolation of viable embryo sacs and their protoplasts of Nicotiana tabacum. Acta Bot Sin , 27: 337-344
|
| 33 |
Hu S Y, Yang H Y (2002). Biology of Angiosperm Fertilization.Beijing: Science Press, 250
|
| 34 |
Hu T X, Yu M, Zhao J (2010). Comparative transcriptional profiling analysis of the two daughter cells from tobacco zygote reveals the transcriptome differences in the apical and basal cells. BMC Plant Biol , 10(1): 167
doi: 10.1186/1471-2229-10-167 pmid:20699003
|
| 35 |
Huang B Q, Russell S D (1989). Isolation of fixed and viable eggs, central cells, and embryo sacs from ovules of Plumbago zeylanica. Plant Physiol , 90(1): 9-12
doi: 10.1104/pp.90.1.9 pmid:16666774
|
| 36 |
Ito Y, Chujo A, Eiguchi M, Kurata N (2004). Radial axis differentiation in a globular embryo is marked by HAZ1, a PHD-finger homeobox gene of rice. Gene , 331: 9-15
doi: 10.1016/j.gene.2004.02.040 pmid:15094187
|
| 37 |
Johnston A J, Meier P, Gheyselinck J, Wuest S E, Federer M, Schlagenhauf E, Becker J D, Grossniklaus U (2007). Genetic subtraction profiling identifies genes essential for Arabidopsis reproduction and reveals interaction between the female gametophyte and the maternal sporophyte. Genome Biol , 8(10): R204
doi: 10.1186/gb-2007-8-10-r204 pmid:17915010
|
| 38 |
Jones-Rhoades M W, Borevitz J O, Preuss D (2007). Genome-wide expression profiling of the Arabidopsis female gametophyte identifies families of small, secreted proteins. PLoS Genet , 3(10): 1848-1861
doi: 10.1371/journal.pgen.0030171 pmid:17937500
|
| 39 |
Karrer E E, Lincoln J E, Hogenhout S, Bennett A B, Bostock R M, Martineau B, Lucas W J, Gilchrist D G, Alexander D (1995). In situ isolation of mRNA from individual plant cells: creation of cell-specific cDNA libraries. Proc Natl Acad Sci U S A , 92(9): 3814-3818
doi: 10.1073/pnas.92.9.3814 pmid:7731989
|
| 40 |
Katoh N, L?rz H, Kranz E (1997). Isolation of viable egg cells of rape (Brassica napus L.). Zygote , 5(1): 31-33
doi: 10.1017/S0967199400003531 pmid:9223243
|
| 41 |
Kerk N M, Ceserani T, Tausta S L, Sussex I M, Nelson T M (2003). Laser capture microdissection of cells from plant tissues. Plant Physiol , 132(1): 27-35
doi: 10.1104/pp.102.018127 pmid:12746508
|
| 42 |
Kranz E, Bautor J, L?rz H (1991). In vitro fertilization of single, isolated gametes of maize mediated by electrofusion. Sex Plant Reprod , 4: 12-16
doi: 10.1007/BF00194565
|
| 43 |
Kumlehn J, L?rz H, Kranz E (1998). Differentiation of isolated wheat zygotes into embryos and normal plants. Planta , 205(3): 327-333
doi: 10.1007/s004250050327
|
| 44 |
Kumlehn K, Czihal A, Matzk L (2001). Parthenogenetic egg cells of wheat: cellular and molecular studies. Sex Plant Reprod , 14(4): 239-243
doi: 10.1007/s00497-001-0115-3
|
| 45 |
Le B H, Cheng C, Bui A Q, Wagmaister J A, Henry K F, Pelletier J, Kwong L, Belmonte M, Kirkbride R, Horvath S, Drews G N, Fischer R L, Okamuro J K, Harada J J, Goldberg R B (2010). Global analysis of gene activity during Arabidopsis seed development and identification of seed-specific transcription factors. Proc Natl Acad Sci USA , 107(18): 8063-8070
doi: 10.1073/pnas.1003530107 pmid:20385809
|
| 46 |
Le B H, Wagmaister J A, Kawashima T, Bui A Q, Harada J J, Goldberg R B (2007). Using genomics to study legume seed development. Plant Physiol , 144(2): 562-574
doi: 10.1104/pp.107.100362 pmid:17556519
|
| 47 |
Lê Q, Gutièrrez-Marcos J F, Costa L M, Meyer S, Dickinson H G, L?rz H, Kranz E, Scholten S (2005). Construction and screening of subtracted cDNA libraries from limited populations of plant cells: a comparative analysis of gene expression between maize egg cells and central cells. Plant J , 44(1): 167-178
doi: 10.1111/j.1365-313X.2005.02518.x pmid:16167904
|
| 48 |
Lee J M, Williams M E, Tingey S V, Rafalski J A (2002). DNA array profiling of gene expression changes during maize embryo development. Funct Integr Genomics , 2(1-2): 13-27
doi: 10.1007/s10142-002-0046-6 pmid:12021847
|
| 49 |
Loader N M, Woolner E M, Hellyer A, Slabas A R, Safford R (1993). Isolation and characterization of two Brassica napus embryo acyl-ACP thioesterase cDNA clones. Plant Mol Biol , 23(4): 769-778
doi: 10.1007/BF00021532 pmid:8251630
|
| 50 |
Márton M L, Cordts S, Broadhvest J, Dresselhaus T (2005). Micropylar pollen tube guidance by egg apparatus 1 of maize. Science , 307(5709): 573-576
doi: 10.1126/science.1104954 pmid:15681383
|
| 51 |
Nakazono M, Qiu F, Borsuk L A, Schnable P S (2003). Laser-capture microdissection, a tool for the global analysis of gene expression in specific plant cell types: identification of genes expressed differentially in epidermal cells or vascular tissues of maize. Plant Cell , 15(3): 583-596
doi: 10.1105/tpc.008102 pmid:12615934
|
| 52 |
Nelson T, Tausta S L, Gandotra N, Liu T (2006). Laser microdissection of plant tissue: what you see is what you get. Annu Rev Plant Biol , 57(1): 181-201
doi: 10.1146/annurev.arplant.56.032604.144138 pmid:16669760
|
| 53 |
Ning J, Peng X B, Qu L H, Xin H P, Yan T T, Sun M (2006). Differential gene expression in egg cells and zygotes suggests that the transcriptome is restructed before the first zygotic division in tobacco. FEBS Lett , 580(7): 1747-1752
doi: 10.1016/j.febslet.2006.02.028 pmid:16510144
|
| 54 |
Okada T, Bhalla P L, Singh M B (2006). Expressed sequence tag analysis of Lilium longiflorum generative cells. Plant Cell Physiol , 47(6): 698-705
doi: 10.1093/pcp/pcj040 pmid:16571618
|
| 55 |
Okada T, Singh M B, Bhalla P L (2007). Transcriptome profiling of Lilium longiflorum generative cells by cDNA microarray. Plant Cell Rep , 26(7): 1045-1052
doi: 10.1007/s00299-006-0300-9 pmid:17245599
|
| 56 |
Okamoto T, Higuchi K, Shinkawa T, Isobe T, L?rz H, Koshiba T, Kranz E (2004). Identification of major proteins in maize egg cells. Plant Cell Physiol , 45(10): 1406-1412
doi: 10.1093/pcp/pch161 pmid:15564524
|
| 57 |
Okamoto T, Scholten S, L?rz H, Kranz E (2005). Identification of genes that are up- or down-regulated in the apical or basal cell of maize two-celled embryos and monitoring their expression during zygote development by a cell manipulation- and PCR-based approach. Plant Cell Physiol , 46(2): 332-338
doi: 10.1093/pcp/pci032 pmid:15695436
|
| 58 |
Okuda S, Tsutsui H, Shiina K, Sprunck S, Takeuchi H, Yui R, Kasahara R D, Hamamura Y, Mizukami A, Susaki D, Kawano N, Sakakibara T, Namiki S, Itoh K, Otsuka K, Matsuzaki M, Nozaki H, Kuroiwa T, Nakano A, Kanaoka M M, Dresselhaus T, Sasaki N, Higashiyama T (2009). Defensin-like polypeptide LUREs are pollen tube attractants secreted from synergid cells. Nature , 458(7236): 357-361
doi: 10.1038/nature07882 pmid:19295610
|
| 59 |
Pina C, Pinto F, Feijó J A, Becker J D (2005). Gene family analysis of the Arabidopsis pollen transcriptome reveals biological implications for cell growth, division control, and gene expression regulation. Plant Physiol , 138(2): 744-756
doi: 10.1104/pp.104.057935 pmid:15908605
|
| 60 |
Ranford J C, Bryce J H, Morris P C (2002). PM19, a barley (Hordeum vulgare L.) gene encoding a putative plasma membrane protein, is expressed during embryo development and dormancy. J Exp Bot , 53(366): 147-148
doi: 10.1093/jexbot/53.366.147 pmid:11741051
|
| 61 |
Russell S D (1986). Isolation of sperm cells from the pollen of Plumbago zeylanica. Plant Physiol , 81(1): 317-319
doi: 10.1104/pp.81.1.317 pmid:16664799
|
| 62 |
Russell S D (1992). Double fertilization. Int Rev Cytol , 40: 357-390
doi: 10.1016/S0074-7696(08)61102-X
|
| 63 |
Sanger F (1988). Sequences, sequences, and sequences. Annu Rev Biochem , 57(1): 1-28
doi: 10.1146/annurev.bi.57.070188.000245 pmid:2460023
|
| 64 |
Shendure J, Ji H (2008). Next-generation DNA sequencing. Nat Biotechnol , 26(10): 1135-1145
doi: 10.1038/nbt1486 pmid:18846087
|
| 65 |
Simone N L, Bonner R F, Gillespie J W, Emmert-Buck M R, Liotta L A (1998). Laser-capture microdissection: opening the microscopic frontier to molecular analysis. Trends Genet , 14(7): 272-276
doi: 10.1016/S0168-9525(98)01489-9 pmid:9676529
|
| 66 |
Spencer M W, Casson S A, Lindsey K (2007). Transcriptional profiling of the Arabidopsis embryo. Plant Physiol , 143(2): 924-940
doi: 10.1104/pp.106.087668 pmid:17189330
|
| 67 |
Sprunck S, Baumann U, Edwards K, Langridge P, Dresselhaus T (2005). The transcript composition of egg cells changes significantly following fertilization in wheat (Triticum aestivum L.). Plant J , 41(5): 660-672
doi: 10.1111/j.1365-313X.2005.02332.x pmid:15703054
|
| 68 |
Steffen J G, Kang I H, Macfarlane J, Drews G N (2007). Identification of genes expressed in the Arabidopsis female gametophyte. Plant J , 51(2): 281-292
doi: 10.1111/j.1365-313X.2007.03137.x pmid:17559508
|
| 69 |
Stinson J R, Eisenberg A J, Willing R P, Pe M E, Hanson D D, Mascarenhas J P (1987). Genes expressed in the male gametophyte of flowering plants and their isolation. Plant Physiol , 83(2): 442-447
doi: 10.1104/pp.83.2.442 pmid:16665265
|
| 70 |
Tanaka I (1988). Isolation of generative cells and their protoplasts from pollen of Lilium longiflorum. Protoplasma , 142(1): 68-73
doi: 10.1007/BF01273227
|
| 71 |
Tang F, Barbacioru C, Wang Y, Nordman E, Lee C, Xu N, Wang X, Bodeau J, Tuch B B, Siddiqui A, Lao K, Surani M A (2009). mRNA-Seq whole-transcriptome analysis of a single cell. Nat Methods , 6(5): 377-382
doi: 10.1038/nmeth.1315 pmid:19349980
|
| 72 |
Theerakulpisut P, Xu H, Singh M B, Pettitt J M, Knox R B (1991). Isolation and developmental expression of Bcp1, an anther-specific cDNA clone in Brassica campestris. Plant Cell , 3(10): 1073-1084
pmid:1821760
|
| 73 |
Twell D, Wing R, Yamaguchi J, McCormick S (1989). Isolation and expression of an anther-specific gene from tomato. Mol Gen Genet , 217(2-3): 240-245
doi: 10.1007/BF02464887 pmid:2770694
|
| 74 |
Wall P K, Leebens-Mack J, Chanderbali A S, Barakat A, Wolcott E, Liang H, Landherr L, Tomsho L P, Hu Y, Carlson J E, Ma H, Schuster S C, Soltis D E, Soltis P S, Altman N, dePamphilis C W (2009). Comparison of next generation sequencing technologies for transcriptome characterization. BMC Genomics , 10(1): 347
doi: 10.1186/1471-2164-10-347 pmid:19646272
|
| 75 |
Wang D, Zhang C, Hearn D J, Kang I H, Punwani J A, Skaggs M I, Drews G N, Schumaker K S, Yadegari R (2010). Identification of transcription-factor genes expressed in the Arabidopsis female gametophyte. BMC Plant Biol , 10(1): 110
doi: 10.1186/1471-2229-10-110 pmid:20550711
|
| 76 |
Wang D Y, Zhang Q, Liu Y, Lin Z F, Zhang S X, Sun M X, Sodmergen (2010). The levels of male gametic mitochondrial DNA are highly regulated in angiosperms with regard to mitochondrial inheritance. Plant Cell , 22(7): 2402-2416
doi: 10.1105/tpc.109.071902 pmid:20605854
|
| 77 |
Wei L Q, Xu W Y, Deng Z Y, Su Z, Xue Y, Wang T (2010). Genome-scale analysis and comparison of gene expression profiles in developing and germinated pollen in Oryza sativa. BMC Genomics , 11(1): 338
doi: 10.1186/1471-2164-11-338 pmid:20507633
|
| 78 |
Weterings K, Apuya N R, Bi Y, Fischer R L, Harada J J, Goldberg R B (2001). Regional localization of suspensor mRNAs during early embryo development. Plant Cell , 13(11): 2409-2425
pmid:11701878
|
| 79 |
Whittle C A, Malik M R, Li R, Krochko J E (2010). Comparative transcript analyses of the ovule, microspore, and mature pollen in Brassica napus. Plant Mol Biol , 72(3): 279-299
doi: 10.1007/s11103-009-9567-x pmid:19949835
|
| 80 |
Wuest S E, Vijverberg K, Schmidt A, Weiss M, Gheyselinck J, Lohr M, Wellmer F, Rahnenführer J, von Mering C, Grossniklaus U (2010). Arabidopsis female gametophyte gene expression map reveals similarities between plant and animal gametes. Curr Biol , 20(6): 506-512
doi: 10.1016/j.cub.2010.01.051 pmid:20226671
|
| 81 |
Xin H P, Peng X B, Ning J, Yan T T, Ma L G, Sun M X (2010). Expressed sequence-tag analysis of tobacco sperm cells reveals a unique transcriptional profile and selective persistence of paternal transcripts after fertilization. Sex Plant Reprod ,
doi: 10.1007/s00497-010-0151-y pmid:20981558
|
| 82 |
Xu H, Swoboda I, Bhalla P L, Sijbers A M, Zhao C, Ong E K, Hoeijmakers J H, Singh M B (1998). Plant homologue of human excision repair gene ERCC1 points to conservation of DNA repair mechanisms. Plant J , 13(6): 823-829
doi: 10.1046/j.1365-313X.1998.00081.x pmid:9681020
|
| 83 |
Xu H, Swoboda I, Bhalla P L, Singh M B (1999). Male gametic cell-specific gene expression in flowering plants. Proc Natl Acad Sci USA , 96(5): 2554-2558
doi: 10.1073/pnas.96.5.2554 pmid:10051681
|
| 84 |
Xu H, Weterings K, Vriezen W, Feron R, Xue Y, Derksen J, Mariani C (2002). Isolation and characterization of male-germ-cell transcripts in Nicotiana tabacum. Sex Plant Reprod , 14(6): 339-346
doi: 10.1007/s00497-002-0128-6
|
| 85 |
Yang H, Kaur N, Kiriakopolos S, McCormick S (2006). EST generation and analyses towards identifying female gametophyte-specific genes in Zea mays L. Planta , 224(5): 1004-1014
doi: 10.1007/s00425-006-0283-3 pmid:16718485
|
| 86 |
Yu H J, Hogan P, Sundaresan V (2005). Analysis of the female gametophyte transcriptome of Arabidopsis by comparative expression profiling. Plant Physiol , 139(4): 1853-1869
doi: 10.1104/pp.105.067314 pmid:16299181
|
| 87 |
Zhang G, Guo G, Hu X, Zhang Y, Li Q, Li R, Zhuang R, Lu Z, He Z, Fang X, Chen L, Tian W, Tao Y, Kristiansen K, Zhang X, Li S, Yang H, Wang J, Wang J (2010). Deep RNA sequencing at single base-pair resolution reveals high complexity of the rice transcriptome. Genome Res , 20(5): 646-654
doi: 10.1101/gr.100677.109 pmid:20305017
|
| 88 |
Zhang Z, Xu H, Singh M B, Russell S D (1998). Isolation and collection of two populations of viable sperm cells from the pollen of Plumbago zeylanica. Zygote , 6(4): 295-298
doi: 10.1017/S0967199498000240 pmid:9921639
|
| 89 |
Zhao J, Zhou C, Yang H Y (2000). Isolation and in vitro culture of zygotes and central cells of Oryza sativa L. Plant Cell Rep , 19(3): 321-326
doi: 10.1007/s002990050020
|
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