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Frontiers in Biology

ISSN 1674-7984

ISSN 1674-7992(Online)

CN 11-5892/Q

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Front. Biol.    2014, Vol. 9 Issue (4) : 283-286    https://doi.org/10.1007/s11515-014-1315-1
RESEARCH ARTICLE
Age-related peculiarities of change in content of free radical oxidation products in muscle during stress
Vadim V. DAVYDOV1,*(),Amjad HAMDALLAH2,Evgenya R. GRABOVETSKAYA3
1. Laboratory of Age Biochemistry State Institution Institute of Children and Adolescent Health NAMS of Ukraine, Kharkov, 61154, Ukraine
2. Chair of biochemistry V.N. Karazin Kharkov National University, Kharkov, 61077, Ukraine
3. Chair of medical chemistry Kharkov National Medical University, Kharkov, 61022, Ukraine
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Abstract

The age-dependent peculiarities of stimulation of free radical processes in subcellular fractions of skeletal muscle of rats subjected to long-term immobilization stress were studied in order to improve knowledge about changes of muscular tissue during ontogenesis. It is found that adult animals do not show accumulation of proteins carbonyls, TBA-reactive substances, and Schiff bases in subcellular fractions of the thigh muscle when immobilized. Long-term immobilization causes apparent manifestation of oxidative stress only in mitochondrial fraction in pubertal rats. Mitochondrial oxidative stress defense systems are sufficiently effective, however, direction of pathways of free radical oxidation carbonyl products catabolism alters in the cytoplasm of myocytes in old rats under long-term immobilization conditions.
Keywords ontogenesis      stress      skeletal muscle      free radical oxidation     
Corresponding Author(s): Vadim V. DAVYDOV   
Issue Date: 11 August 2014
 Cite this article:   
Vadim V. DAVYDOV,Amjad HAMDALLAH,Evgenya R. GRABOVETSKAYA. Age-related peculiarities of change in content of free radical oxidation products in muscle during stress[J]. Front. Biol., 2014, 9(4): 283-286.
 URL:  
https://academic.hep.com.cn/fib/EN/10.1007/s11515-014-1315-1
https://academic.hep.com.cn/fib/EN/Y2014/V9/I4/283
Fig.1  Ratio Shiff bases / TBA reactive substances (ShB/TBA + ) in mitochondrial and postmitochondrial fraction of femoral muscle during stress (mean ± SEM). The figure represents the average data from 6 investigations.

* The data are positively distinguished from intact rats (p < 0.05).

Age (months)Experimental group of ratsProtein carbonyls (μmol/mg protein)TBA + substances (μmol/mg protein)Shiff Bases (nmol/mg protein)
1.5intact0.77 ± 0.120.16 ± 0.030.49 ± 0.06
immobilized1.34 ± 0.02**0.15 ± 0.030.95 ± 0.04**
12intact1.90 ± 0.27*0.23 ± 0.02*0.94 ± 0.03*
immobilized1.39 ± 0.290.24 ± 0.030.59 ± 0.06**
24intact1.20 ± 0.390.09 ± 0.011.08 ± 0.05*
immobilized0.56 ± 0.080.07 ± 0.011.08 ± 0.14
Tab.1  Level products of free radical oxidation in mitochondrial fraction of femoral muscle during stress (mean ± SEM)
Age (months)Experimental group of ratsProtein Carbonyls (μmol/mg protein)TBA + substances (μmol/mg protein)Shiff Bases (nmol/mg protein)
1.5intact0.61 ± 0.140.045 ± 0.0060.48 ± 0.04
immobilized0.62 ± 0.080.045 ± 0.0080.70 ± 0.09
12intact0.60 ± 0.010.068 ± 0.0200.38 ± 0.02
immobilized0.64 ± 0.010.028 ± 0.004**0.29 ± 0.03**
24intact0.57 ± 0.130.050 ± 0.0160.23 ± 0.02*
immobilized0.73 ± 0.130.025 ± 0.0050.20 ± 0.03
Tab.2  Level products of free radical oxidation in postmitochondrial fraction of femoral muscle during stress (mean ± SEM)
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