Bioactive compounds from marine <i>Streptomyces</i>sp<i>.</i> VITPSA as therapeutics

doi:10.1007/s11515-017-1459-x

Front. Biol.

2017, Vol. 12

Issue (4) : 280-289 https://doi.org/10.1007/s11515-017-1459-x

RESEARCH ARTICLE

Bioactive compounds from marine Streptomycessp. VITPSA as therapeutics

S. Pooja, T. Aditi, S. Jemimah Naine, C. Subathra Devi(

)

School of Biosciences and Technology, VIT University, Tamil Nadu, India

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Abstract

BACKGROUND: Marine actinomycetes are efficient producers of new secondary metabolites that show different biological activities, including antibacterial, antifungal, anticancer, insecticidal, and enzyme inhibition activities.

METHODS: The morphological, physiological, and biochemical properties of the strain Streptomyces sp. VITPSA were confirmed by conventional methods. Antibacterial, anti-oxidant, anti-inflammatory, anti-diabetic, and cytotoxic activities of Streptomyces sp. VITPSA extract were determined. The media were optimized for the production of secondary metabolites. Characterization and identification of secondary metabolites were conducted by high-performance liquid chromatography, gas chromatography-mass spectroscopy, and Fourier transform infrared spectroscopy analysis.

RESULTS: The strain showed significant antibacterial, anti-oxidant, and cytotoxic activities, moderate anti-inflammatory activity, and no satisfactory anti-diabetic activity. The ethyl acetate extract of Streptomyces sp. VITPSA showed maximum antibacterial activity against two gram-positive and gram-negative bacteria at 0.5 mg/mL. The antioxidant potential of the crude extract exhibited strong reducing power activity at 0.5 mg/mL with 95.1% inhibition. The cytotoxic effect was found to be an IC₅₀ of 50 µg/mL on MCF-7 cell lines. Experimental design of optimization by one-factor analysis revealed the most favorable sucrose, yeast extract, pH (7.25), and temperature (28°C) conditions for the effective production of secondary metabolites.

CONCLUSION: This study revealed that Streptomycessp. VITPSA is an excellent source of secondary metabolites with various bioactivities.

Keywords Marine actinomycetes bioactive compounds pathogens oxidants

Corresponding Author(s): C. Subathra Devi

Online First Date: 05 September 2017 Issue Date: 13 September 2017

Cite this article:

S. Pooja,T. Aditi,S. Jemimah Naine, et al. Bioactive compounds from marine Streptomycessp. VITPSA as therapeutics[J]. Front. Biol., 2017, 12(4): 280-289.

URL:

https://academic.hep.com.cn/fib/EN/10.1007/s11515-017-1459-x
https://academic.hep.com.cn/fib/EN/Y2017/V12/I4/280

Fig.1 (A) Streak plate showing pure culture of Streptomyces sp. VITPSA on starch casein agar medium

(B) Microscopy 100X: showing morphology of Streptomyces sp. VITPSA

Fig.2 Phylogenetic tree showing relationship of Streptomyces sp. VITPSA to other species

Fig.3 Antibacterial activity of crude extract compared with positive control

Fig.4 Radical scavenging activity of crude extract

Fig.5 Anti-inflammatory activity of Streptomyces sp. VITPSA crude extract

Fig.6 Anti-diabetic activity of ethyl acetate extract of Streptomyces sp. VITPSA

Fig.7 (a) Graphical representation of cytotoxic test activity; (b) Cytotoxic test results at different concentrations on MCF7 cell lines

Fig.8 TLC plate showing spots

Fig.9 HPLC chromatogram for ethyl acetate extract of Streptomyces sp. VITPSA

Fig.10 GC-MS chromatogram for ethyl acetate extract of Streptomyces sp. VITPSA

Fig.11 IR spectra for ethyl acetate extract of Streptomyces sp. VITPSA extract

Fig.12 UV analysis spectrum of Streptomyces sp. VITPSA extract

Fig.13

Tab.1 Table 2Drug likeliness based on Lipinski rule of five

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