Process optimization of benzo[ghi]perylene biodegradation by yeast consortium in presence of ZnO nanoparticles and produced biosurfactant using Box-Behnken design

doi:10.1007/s11515-018-1523-1

Front. Biol.

2018, Vol. 13

Issue (6) : 418-424 https://doi.org/10.1007/s11515-018-1523-1

RESEARCH ARTICLE

Process optimization of benzo[ghi]perylene biodegradation by yeast consortium in presence of ZnO nanoparticles and produced biosurfactant using Box-Behnken design

Sanjeeb Kumar Mandal, Nupur Ojha, Nilanjana Das(

)

Bioremediation Laboratory, Department of Bio-Medical Sciences, School of Bio Sciences and Technology, VIT (Vellore Institute of Technology), Vellore-632014, Tamil Nadu, India

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Abstract

BACKGROUND: Benzo[ghi]perylene (BghiP), a polycyclic aromatic hydrocarbon (PAH) containing six fused benzene rings is considered as priority pollutant because of its carcinogenicity, mutagenicity and acute toxicity.

METHODS: The synthesis of ZnO nanoparticles was done following the standard method. Biosurfactant production by yeast consortium YC04 in MSM was confirmed by various tests viz. drop collapse test, methylene blue agar plate method and emulsification test (E24) using the standard procedures. Efficiency of YC04 was tested to remediate BghiP in presence of ZnO nanoparticles and produced biosurfactant in the growth medium.

RESULTS: Response surface methodology (RSM), 3-level five variables Box-Behnken design (BBD) was employed to optimize the factors viz. pH 7.0, temperature 30°C, shaking speed 130 rpm, inoculum dosage 3% and ZnO nanoparticles concentration 2 g/L after a period of 6 days of incubation for the enhanced degradation of BghiP (63.83±0.01%). It was well in close agreement with the predicated value obtained by RSM model yield (63.83±0.08%). Analysis of variance (ANOVA) showed F-value of 51.70, R2 of 0.9764, probability of<0.0001 and coefficient of variation of 1.25% confirmed the validity of the model. Degradation of BghiP was assessed using GC-MS and FTIR analysis. Kinetic study demonstrated that BghiP degradation fitted first order kinetic model.

CONCLUSIONS: To the best of our knowledge, this is the first report on process optimization toward nanobioremediation of BghiP using yeast consortium in presence of ZnO nanoparticles and produced biosurfactant in medium.

Keywords biodegradation bioremediation optimization pollutants yeasts

Corresponding Author(s): Nilanjana Das

Online First Date: 26 October 2018 Issue Date: 30 November 2018

Cite this article:

Sanjeeb Kumar Mandal,Nupur Ojha,Nilanjana Das. Process optimization of benzo[ghi]perylene biodegradation by yeast consortium in presence of ZnO nanoparticles and produced biosurfactant using Box-Behnken design[J]. Front. Biol., 2018, 13(6): 418-424.

URL:

https://academic.hep.com.cn/fib/EN/10.1007/s11515-018-1523-1
https://academic.hep.com.cn/fib/EN/Y2018/V13/I6/418

Fig.1 Biodegradation studies of benzo[ghi]perylene (BghiP) using yeast consortium YC04 through instrumental analysis. (A) GC-MS analysis of BghiP degradation under different sets of condition after 6 days of incubation; (B) FT-IR spectrum of BghiP before and after degradation in pres-ence of ZnO nanoparticle and biosurfactant.

Fig.2 3-D interactions between the different variables for the response (BghiP biodegradation %). (A) pH vs. ZnO nanoparticles (AE); (B) Tem-perature vs. Dosage (BD); (C) Shaking speed vs. ZnO nanoparticles (CE); (D) D. Dosage vs. ZnO nanoparticles (DE); (E) Normal plot of residuals; (F) Predicted vs. actual plot.

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