Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells
Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells
LI Yahui1, DONG Shiyuan1, YU Chao1, JIANG Yu1, LI Huaixing2, SUN Shuhan3
1.Center for Healthy Food Evaluation, State Food and Drug Administration, Beijing 100061, China; 2.Institute for Nutritional Science, Shanghai Institute for Biological Sciences, Chinese Academy of Science, Shanghai 200031, China; 3.Department of Medical Genetics, College of Basic Medical Science, Second Military Medical University, Shanghai 200433, China
Abstract：The aim of this article was to investigate the influence and the related mechanism of the Retn gene on glucose uptake and insulin resistance in 3T3-L1 cells. Radioimmunoassay was used to determine glucose uptake in 3T3-L1 cells with different Retn gene expression levels, whether cells were stimulated by insulin or not. RT-PCR and real-time RT-PCR analysis were used to determine the mRNA levels of several glucose transport proteins in 3T3-L1 cells with different Retn gene expression levels, including insulin receptor substrate-1(IRS-1), phosphatidylinositol 3-kinase (PI-3K), AKT-2, glucose transporter-4 (GLUT-4), p38 mitogen-activated protein kinase (p38MAPK) and glycogen synthase kinase-3b (GSK-3β). The glucose uptake decreased with the increase in Retn gene expression in 3T3-L1 cells, which was independent of whether the cells were stimulated by insulin or not. The mRNA expression of two signal proteins PI-3K and AKT-2 decreased and the other two signal proteins, GSK-3β and p38MAPK, increased with Retn overexpression in 3T3-L1 cells. Resistin could induce insulin resistance in adipocytes, which might be related to the changes of some proteins in PI-3K and Ras pathways.
出版日期: 2007-09-05
引用本文:
. Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells[J]. Frontiers of Medicine in China, 2007, 1(3): 269-273.
LI Yahui, DONG Shiyuan, YU Chao, JIANG Yu, LI Huaixing, SUN Shuhan. Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells. Front. Med., 2007, 1(3): 269-273.
