Inhibition of NF-kappa B can enhance Fas-mediated apoptosis in leukemia cell line HL-60
Inhibition of NF-kappa B can enhance Fas-mediated apoptosis in leukemia cell line HL-60
Li WANG1, Shi ZHAO1,2(), Hong-Xiang WANG1, Ping ZOU2
1. Department of Hematology, The Central Hospital of Wuhan, Wuhan 430014, China; 2. Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
This study explored the effects of nuclear factor-kappa B (NF-κB) inhibitor Bay 11-7082 on Fas/FasL system and Fas-mediated apoptosis in cell line HL-60 cells. The mRNA and protein levels of Fas, FasL, and X-linked inhibitor of apoptosis protein (XIAP) were detected by reverse transcription-polymerase chain reaction (RT-PCR) and flow cytometry (FCM); the level of sFasL was evaluated by enzyme-linked immunosorbent assay (ELISA); and apoptosis was determined by FCM. After treatment with Bay 11-7082, the mRNA and protein levels of FasL and XIAP in HL-60 cells were significantly lower than in the controls (P<0.05), but the mRNA and protein levels of Fas and sFasL did not change significantly (P>0.05). Apoptotic rate of HL-60 cells treated with Bay 11-7082 was significantly higher than in the controls (P<0.05). Therefore, we conclude that Bay 11-7082 can enhance Fas-mediated apoptosis in HL-60 cells by downregulating FasL and XIAP levels.
. Inhibition of NF-kappa B can enhance Fas-mediated apoptosis in leukemia cell line HL-60[J]. Frontiers of Medicine in China, 2010, 4(3): 323-328.
Li WANG, Shi ZHAO, Hong-Xiang WANG, Ping ZOU. Inhibition of NF-kappa B can enhance Fas-mediated apoptosis in leukemia cell line HL-60. Front Med Chin, 2010, 4(3): 323-328.
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