|
|
|
An in vitro study of femtosecond laser photodisruption
in rabbit sclera |
| JIANG Fagang1, YANG Xiaobo1, CUI Ling1, DAI Nengli2, LU Peixiang2, LONG Hua2 |
| 1.Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology; 2.State Key Laboratory of Laser Technology, Huazhong University of Science and Technology |
|
|
|
|
Abstract To explore the possibility of photodisruption in rabbit sclera by femtosecond (fs) laser and seek appropriate incision techniques and relevant parameters, a fs laser (800 nm/50 fs) with different pulse energies was applied to irradiate rabbit sclera in vitro. By moving a computer-controlled three-axis translation stage to which the sample was attached, the laser achieved three types of incisions: transscleral channel, snake pattern and linear cut. The irradiated samples were observed by light microscopy and scanning electron microscopy (SEM). In comparison with fs laser, Nd:YAG was used as control. The experimental results show that through an objective lens with numerical aperture (NA) of 0.2, the fs laser with power intensity larger than 955 TW/cm2 and pulse energy ranging from 37.5–125 ?J, cutting depths from 30–70 ?m may be achieved after linearly scanning in sclera at a translation speed of 0.1 mm/s. However, it failed to make any photodisruption when the power intensity was below 796 TW/cm2 or the pulse energy was less than 31.25 ?J under the same condition. Compared with the Nd:YAG laser, the inner wall of the channel was smoother and the damage to the surrounding tissues was slight with the fs laser. The high precision of intrascleral photodisruption and minimal damage to surrounding tissues by a fs laser display its potential application in the treatment of glaucoma.
|
|
Issue Date: 05 June 2008
|
|
| 1 |
Toyran S, Liu Y M, Singha S, et al.. Femtosecond laser photodisruption of human trabecularmeshwork: an in vitro study. ExperimentalEye Research, 2005, 81(3): 298–305
|
| 2 |
Ngoi B K, Hou D X, Koh L H, et al.. Femtosecond laser for glaucoma treatment: astudy on ablation energy in pig iris. Lasersin Medical Science, 2005, 19(4): 218–222.
doi:10.1007/s10103-004-0323-9
|
| 3 |
Juhasz T, Kastis G A, Suarez C, et al.. Time-resolved observations of shock waves andcavitation bubbles generated by femtosecond laser pulses in cornealtissue and water. Lasers in Surgery andMedicine, 1996, 19(1): 23–31.
doi:10.1002/(SICI)1096-9101(1996)19:1<23::AID-LSM4>3.0.CO;2-S
|
| 4 |
Schwab B, Hagner D, Müller W, et al.. Bone ablation using ultrashort laser pulses.A new technique for middle ear surgery. Laryngorhinootologie, 2004, 83(4): 219–225.
doi:10.1055/s-2004-814270
|
| 5 |
Binder P S . Flap dimensions created with the IntraLase FS laser. Journal of Cataract and Refractive Surgery, 2004, 30(1): 26–32.
doi:10.1016/S0886-3350(03)00578-9
|
| 6 |
Lubatschowski H, Maatz G, Heisterkamp A, et al.. Application of ultra-short laser pulses forintrastromal refractive surgery. Graefe'sArchive for Clinical and Experimental Ophthalmology, 2000, 238(1): 33–39.
doi:10.1007/s004170050006
|
| 7 |
Sacks Z S, Kurtz R M, Juhasz T, et al.. High precision subsurface photodisruption inhuman sclera. Journal of Biomedical Optics, 2002, 7(3): 442–450.
doi:10.1117/1.1482381
|
| 8 |
Wang X F, Jia T Q, Li X X, et al.. Ablation and ultrafast dynamics of zinc selenideunder femtosecond laser irradiation. ChineseOptics Letters, 2005, 3(10): 615–617
|
| 9 |
Sacks Z S, Kurtz R M, Juhasz T, et al.. Subsurface photodisruption in human sclera:wavelength dependence. Ophthalmic Surgery,Lasers & Imaging, 2003, 34(2): 104–113
|
| 10 |
Chen H X, Jia T Q, Huang M, et al.. Visible-infrared femtosecond laser-induced opticalbreakdown of 6H SiC. Acta Optica Sinica, 2006, 26(3): 468–470 (in Chinese)
|
| 11 |
Niemz M H . Laser-Tissue Interactions Fundamentals and Applications (in Chinese,trans. Zhang Zhenxi). 3rd ed.Beijing: Science Press, 2005, 92–132
|
| 12 |
Xing Q R, Mao F L, Lang L Y, et al.. Experiment research on femtosecond laser cellmicromanipulation system. Chinese Journalof Lasers, 2004, 31(6): 728
|
| 13 |
Frederickson K S, White W E, Wheeland R G, et al.. Precise ablation of skin with reduced collateraldamage using the femtosecond-pulsed, terawatt titanium-sapphire laser. Archives of Dermatology, 1993, 129(8): 989–993.
doi:10.1001/archderm.129.8.989
|
| 14 |
Suhm N, Götz M H, Fischer J P, et al.. Ablation of neural tissue by short-pulsed lasers–atechnical report. Acta Neurochirurgica, 1996, 138(3): 346–349.
doi:10.1007/BF01411747
|
| 15 |
Krueger R R, Kuszak J, Lubatschowski H, et al.. First safety study of femtosecond laser photodisruptionin animal lenses: tissue morphology and cataractogenesis. Journal of Cataract and Refractive Surgery, 2005, 31(12): 2386–2394.
doi:10.1016/j.jcrs.2005.05.034
|
| 16 |
Gerten G, Ripken T, Breitenfeld P, et al.. In vitro and in vivo investigations on the treatmentof presbyopia using femtosecond lasers. Ophthalmologe, 2007, 104(1): 40–46.
doi:10.1007/s00347-006-1400-1
|
| 17 |
Serbin J, Bauer T, Fallnich C, et al.. Femtosecond lasers as novel tool in dental surgery. Applied Surface Science, 2002, 197–198: 737–740.
doi:10.1016/S0169-4332(02)00402-6
|
| 18 |
Niemz M H, Kasenbacher A, Strassl M, et al.. Tooth ablation using a CPA-free thin disk femtosecondlaser system. Applied Physics B, 2004, 79(3): 269–271.
doi:10.1007/s00340-004-1579-2
|
| 19 |
Vogel A, Venugopalan V . Mechanisms of pulsed laserablation of biological tissues. ChemicalReviews, 2003, 103(2): 577–644.
doi:10.1021/cr010379n
|
| 20 |
Stern D, Schoenlein R W, Puliafito C A, et al.. Corneal ablation by nanosecond, picosecond andfemtosecond lasers at 532 and 625 nm. Archivesof Ophthalmology, 1989, 107(4): 587–592
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
