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Spectral imaging technology of epithelial tissue
based on two-photon excited fluorescence and second-harmonic generation |
| LUO Tianshu1, CHEN Jianxin1, ZHUO Shuangmu1, JIANG Xingshan1, Chen Huanglin1, Luo Teng1, ZOU Qilian2 |
| 1.Key Laboratory of Optoelectronics Science and Technology for Medicine, Fujian Normal University; 2.Division of Cell Biology and Genetics, Fujian Medical University; |
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Abstract The layer structures of the esophageal and oral tissues were investigated by using spectral imaging technology based on two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG). Because spectral imaging technology allows a simultaneous record of both the spectra and image, it is capable of identifying the layered structures of the epithelial tissues, including the keratinizing layer, epithelial cell layer and stromal layer in the molecular level, which are strongly correlated to tissue pathology. All this work indicates that this technique has the potential to provide more accurate and comprehensive information for the early pathological diagnosis of tissues with the stratified squamous epithelia.
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Issue Date: 05 June 2008
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