Super-resolution imaging of the dynamic cleavage of intercellular tunneling nanotubes

doi:10.1007/s12200-020-1068-1

Front. Optoelectron.

2020, Vol. 13

Issue (4) : 318-326 https://doi.org/10.1007/s12200-020-1068-1

RESEARCH ARTICLE

Super-resolution imaging of the dynamic cleavage of intercellular tunneling nanotubes

Wanjun GONG, Wenhui PAN, Ying HE, Meina HUANG, Jianguo ZHANG, Zhenyu GU, Dan ZHANG, Zhigang YANG(

), Junle QU

Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China

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Abstract

As a new method of cell–cell communication, tunneling nanotubes (TNTs) play important roles in cell–cell signaling and mass exchanges. However, a lack of powerful tools to visualize dynamic TNTs with high temporal/spatial resolution restricts the exploration of their formation and cleavage, hindering the complete understanding of its mechanism. Herein, we present the first example of using stochastic optical reconstruction microscopy (STORM) to observe the tube-like structures of TNTs linking live cells with an easily prepared fluorescent dye. Because of this new imaging microscopy, the cleavage process of TNTs was observed with a high spatial resolution.

Keywords super-resolution tunneling nanotubes (TNTs) live cell

Corresponding Author(s): Zhigang YANG

Just Accepted Date: 18 September 2020 Online First Date: 29 October 2020 Issue Date: 31 December 2020

Cite this article:

Wanjun GONG,Wenhui PAN,Ying HE, et al. Super-resolution imaging of the dynamic cleavage of intercellular tunneling nanotubes[J]. Front. Optoelectron., 2020, 13(4): 318-326.

URL:

https://academic.hep.com.cn/foe/EN/10.1007/s12200-020-1068-1
https://academic.hep.com.cn/foe/EN/Y2020/V13/I4/318

Fig.1 Diagram of the proposed labeling method for probe 1 on TNTs

Fig.2 Photophysical properties of probe 1 were evaluated. (a) Excitation and emission spectra of probe 1 (black and red curves, respectively), with a laser excitation wavelength of 633 nm. (b) Duty cycle calculated for probe 1. (c) Single-molecule fluorescence time traces measured in the absence of bME and oxygen-scavenging system

Fig.3 Fluorescent imaging of the probe in HeLa cells. The co-localization experiment was conducted by co-incubating 1 (1 mmol/L) (a) with a commercially available dye, membrane tracker-Dioctadecylcarbocyanines (DIO) (1 mmol/L) (b). The merged image (c) and Pr coefficient (d) was obtained by overlapping (a) and (b); FITC is the abbreviation of fluoresceinthioisocyanate. (e) Cell viability experiment on HeLa cells when incubating with probe 1

Fig.4 STORM imaging of TNTs and their dynamic changes. (a) One snapshot (t = 0 s) from a 184-s movie (left) and a reconstructed STORM image from 180 frames recorded over 3 s. (b) and (c) Time-series STORM snapshots of intercellular filament dynamics. Each image was reconstructed from 180 frames recorded over 3 s. (d) Magnified images of the circle in the square in (b) obtained by wide-field and STORM imaging. (e) Fluorescence intensity profiles along the white lines in (d). The black line shows the signal in the STORM image, whereas the blue line shows the signal in the wide-field image. FWHM values of these profiles are 133.5±1.5 nm (super-resolution image) and 530.1±14.1 nm (wide-field image), respectively

Fig. S1 Reversible and spontaneous fluorescence blinking over tens of seconds in the PBS buffer at different times. (a) 10 s. (b) 150 s. (c) 300 s. (d) 450 s. (e) 600 s. (f) 760 s. The bright spots are still observed under long-time illumination by a laser beam (656 nm)

Fig. S2 Wide field imaging (a) of TNTs and STORM imaging of TNTs at different time. (b) 8 s. (c) 40 s. (d) 88 s. (e) 144 s. (f) 196 s

Fig. S3 ¹H NMR spectrum for 1

Fig. S4 ¹³C NMR (300 MHz, CDCl₃) spectra of 1

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