New approaches to diagnostics and treatment of cholangiocellular cancer based on photonics methods

doi:10.1007/s12200-020-1093-0

Front. Optoelectron.

2020, Vol. 13

Issue (4) : 352-359 https://doi.org/10.1007/s12200-020-1093-0

RESEARCH ARTICLE

New approaches to diagnostics and treatment of cholangiocellular cancer based on photonics methods

Dmitry V. YAKOVLEV^1,²(

), Dina S. FARRAKHOVA², Artem A. SHIRYAEV³, Kanamat T. EFENDIEV⁴, Maxim V. LOSCHENOV², Liana M. AMIRKHANOVA³, Dmitry O. KORNEV³, Vladimir V. LEVKIN³, Igor V. RESHETOV³, Victor B. LOSCHENOV^2,⁴

¹. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia
². Prokhorov General Physics Institute of Russian Academy of Sciences, Moscow 119991, Russia
³. University Clinical Hospital No. 1, Oncology Center, I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation, Moscow 119991, Russia
⁴. Department of Laser Micro-, Nano-, and Biotechnology, Institute of Engineering Physics for Biomedicine, National Research Nuclear University MEPhI, Moscow 115409, Russia

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Abstract

Cholangiocellular cancer (CCС) is an oncological disease of the bile ducts characterized by a high mortality rate. To date, the use of standard methods for the diagnosis and treatment of CCС has not been able to reduce mortality from this disease. This work presents the results of fluorescence diagnostics (FD), which consists in using a modified optical fiber and photodynamic therapy (PDT) using a therapeutic laser instead of a low-intensity laser. This technique was tested on 43 patients in a clinical setting. The results obtained indicate a direct correlation between spectroscopic and video FD methods. Furthermore, a direct correlation was found between the photobleaching of a chlorin e6-based photosensitizer, with the commercial names of Photolon Radachlorin and Photoran and stricture regression. Our findings demonstrate the possibility of using a therapeutic laser with a wavelength of 660 nm for both diagnosis and treatment of bile ducts cancer, which results in a significant reduction of the operation time without decreasing its effectiveness.

Keywords cholangiocellular cancer (CCC) fluorescent diagnostics (FD) photodynamic therapy (PDT) chlorin e6 Photolon spectroscopic method video fluorescent method photobleaching cancer bile ducts tumor intraoperation diagnostics photosensitizer (PS)

Corresponding Author(s): Dmitry V. YAKOVLEV

Just Accepted Date: 09 December 2020 Online First Date: 24 December 2020 Issue Date: 31 December 2020

Cite this article:

Dmitry V. YAKOVLEV,Dina S. FARRAKHOVA,Artem A. SHIRYAEV, et al. New approaches to diagnostics and treatment of cholangiocellular cancer based on photonics methods[J]. Front. Optoelectron., 2020, 13(4): 352-359.

URL:

https://academic.hep.com.cn/foe/EN/10.1007/s12200-020-1093-0
https://academic.hep.com.cn/foe/EN/Y2020/V13/I4/352

Fig.1 Scheme 1 (a) Modified optical fiber. (b) Real image of the modified optical fiber

Fig.2 (a) Fluorescence PS spectra normalized to the laser line (l_ex = 660 nm) at the moment of maximum accumulation in the tumor before and after PDT, compared to normal tissue. (b) Histograms of the fluorescence index expressed as the ratio of the area under the chlorin e6 spectral fluorescence curve to the area under the laser radiation curve

Fig.3 Image of the bile duct region with the tumor before PDT. (a) Color mode. (b) Combined mode. The dotted line marks the region of the bile duct tumor. The fluorescence index obtained from the interactive target is displayed in the upper left corner of the image. The fluorescence index outside the tumor is less than 10-rel. units, which corresponds to normal tissue

Fig.4 Image of the bile duct region with the tumor after PDT. (a) Color mode. (b) Combined mode. The dotted line marks the region of the bile duct tumor. The fluorescence index obtained from the interactive target is displayed in the upper left corner of the image

Fig.5 Cholangiographic picture of the bile ducts. (a) Before PDT, a complete contrasting block is observed in the left lobar duct (the arrows indicate the length of the stricture). (b) After PDT, during cholangiography, the contrasting block in the left lobar duct is observed (the arrow indicates recanalization of the left lobar duct)

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