Formation of bone-like apatite on poly(L-lactide) to improve osteoblast-like compatibility in vitro and in vivo

doi:10.1007/s11706-007-0025-x

Front. Mater. Sci.

2007, Vol. 1

Issue (2) : 140-146 https://doi.org/10.1007/s11706-007-0025-x

Formation of bone-like apatite on poly(L-lactide) to improve osteoblast-like compatibility in vitro and in vivo

JIAO Yanpeng¹, LIU Zonghua², ZHOU Changren², CUI Fuzhai³

1.Department of Materials Science & Engineering, Tsinghua University, Beijing 100084, China; Department of Materials Science & Engineering, Jinan University, Guangzhou 510632, China; 2.Department of Materials Science & Engineering, Jinan University, Guangzhou 510632, China; 3.Department of Materials Science & Engineering, Tsinghua University, Beijing 100084, China;

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Abstract The biomimetic apatite coating process was adopted to modify poly(L-lactide) (PLLA) surfaces with osteoblasts-like cell compatibility. The apatite coating was formed on the pre-hydrolyzed PLLA film and scaffold surfaces by incubating in simulated body fluid (SBF). Scanning electron microscopy and energy dispersive X-ray analyzer were utilized to characterize the composition and the structure of the apatite coating. The cytocompatibility of the modified PLLA films was investigated by testing osteoblast-like attachment, proliferation, alkaline phosphatase (ALP) activity, and cell cycle. Subsequently, the modified PLLA scaffolds were co-cultured with the osteoblasts-like in vitro and subcutaneously implanted into nude mice. The experimental results showed that the formed apatite had a nano-sized particle and matrix configuration. The surface modification of PLLA with apatite coating significantly promoted osteoblast-like compatibility. After a four-week culture in vivo, no significant inflammatory signs were observed in the implanted regions and osteoblast-like congeries with bone-like structure began to form in the scaffolds. The positive results of this study suggest a good way to produce desirable PLLA biomaterials for bone tissue engineering.

Issue Date: 05 June 2007

Cite this article:

LIU Zonghua,JIAO Yanpeng,ZHOU Changren, et al. Formation of bone-like apatite on poly(L-lactide) to improve osteoblast-like compatibility in vitro and in vivo[J]. Front. Mater. Sci., 2007, 1(2): 140-146.

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https://academic.hep.com.cn/foms/EN/10.1007/s11706-007-0025-x
https://academic.hep.com.cn/foms/EN/Y2007/V1/I2/140

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