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Frontiers of Materials Science

ISSN 2095-025X

ISSN 2095-0268(Online)

CN 11-5985/TB

Postal Subscription Code 80-974

2018 Impact Factor: 1.701

Front. Mater. Sci.    2008, Vol. 2 Issue (2) : 167-171    https://doi.org/10.1007/s11706-008-0028-2
-linked oligosaccharide in MSP-1 and its implication for scallop calcification
NORIZUKI Michiko, TAKAKURA Daisuke, SAMATA Tetsuro
Laboratory for Cell Biology, Department of Environmental Health, Azabu University;
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Abstract We speculated the structure of the N-linked oligosaccharides enzymatically released from the organic matrix (OM) component in the foliated layer of Patinopecten yessoensis. The 80 kDa component of the soluble OM was detected by lectin blotting and was identified as MSP-1 using liquid chromatography/mass spectrometry (LC/MS/MS). LC/MS/MS analysis of the N-glycan liberated from MSP-1 detected a hybrid-type N-glycan, which contained sulfite and sialic acid at its terminus based on the characteristic Y ions. The data strongly imply that MSP-1, a sulfated OM glycoprotein, participates in molluscan biomineralization by creating a favorable environment for calcium ion uptake through sulfite acid and sialic acid. Further analyses of oligosaccharides linked to the OM components in wide variety of species and shell microstructures may definitely contribute in elucidation of molluscan biomineralization at the molecular level.
Issue Date: 05 June 2008
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TAKAKURA Daisuke,NORIZUKI Michiko,SAMATA Tetsuro. -linked oligosaccharide in MSP-1 and its implication for scallop calcification[J]. Front. Mater. Sci., 2008, 2(2): 167-171.
 URL:  
https://academic.hep.com.cn/foms/EN/10.1007/s11706-008-0028-2
https://academic.hep.com.cn/foms/EN/Y2008/V2/I2/167
1 Sarashina I Endo K The complete primary structureof molluscan shell protein 1 (MSP-1), an acidic glycoprotein in theshell matrix of the scallop Patinopecten yessoensisMarine Biotechnology 2001 3362369.
doi:10.1007/s10126‐001‐0013‐6
2 Miyamoto H Miyashita T Olushima M et al.A carbonic anhydrase from the nacreous layer inoyster pearlsProceedings of the Nationalacademy of Sciences of the United States of America 1996 9396579660.
doi:10.1073/pnas.93.18.9657
3 Sudo S Fujikawa T Nagakura T et al.Structures of mollusk shell framework proteinsNature 1997 387563564.
doi:10.1038/42391
4 Shen X Belcher A M Hansma P K et al.Molecular cloning and characterization of LustrinA, a matrix protein from shell and pearl nacre of Haliotis rufescensThe Journalof Biological Chemistry 1997 2723247232481.
doi:10.1074/jbc.272.51.32472
5 Samata T Hayashi N Kono M et al.A new matrix protein family related to the nacreouslayer formation of Pinctada fucataFEBS Letters 1999 462225229.
doi:10.1016/S0014‐5793(99)01387‐3
6 Kono M Hayashi N Samata T Molecular mechanism of the nacreous layer formation in Pinctada fucataBiochemical and Biophysical Research Communications 2000 269213218.
doi:10.1006/bbrc.2000.2274
7 Mann K Weiss I M Andre S et al.The amino acid sequence of the abalone (Haliotis laevigata) nacre protein perlucindetection of a functional C-type lectin domain with galactose/mannosespecificityEuropean Journal of Biochemistry 2000 27652575264.
doi:10.1046/j.1432‐1327.2000.01602.x
8 Marin F Corstjens P de Gaulejac B et al.Mucins and molluscan calcification. Molecular characterizationof mucoperlin, a novel mucin-like protein from the nacreous shelllayer of the fan mussel Pinna nobilis (bivalvia, pteriomorphia).The Journalof Biological Chemistry 2000 275(27)2066720675.
doi:10.1074/jbc.M003006200
9 Miyashita T Takagi R Okushima M et al.Complementary DNA cloning and characterization ofpearlin a new class of matrix protein in the nacreous layer of oysterpearlsMarine Biotechnology 2000 2409418
10 Weiss I M Gohring W Fritz M et al.Perlustrin, a Haliotis laevigata (abalone) nacre protein, is homologous to the insulin-like growthfactor binding protein N-terminalmodule of vertebratesBiochemical and BiophysicalResearch Communications 2001 285244249.
doi:10.1006/bbrc.2001.5170
11 Miyashita T Takagi R Miyamoto H et al.Identical carbonic anhydrase contributes to nacreousor prismatic layer formation in Pinctada fucata (Mollusca: Bivalvia)The Veliger 2002 45250255
12 Miyamoto H Yano M Miyashita T Similarities in the structure of nacrein the shell-matrixprotein, in a bivalve and a gastropodJournalof Molluscan Studies 2003 69(1)8789.
doi:10.1093/mollus/69.1.87
13 Wada K Studieson the mineralization of calcified tissue in mollusks VII. Histologicaland histochemical studies of organic matrices in shellsBulletin of the National Pearl Research Laboratory 1964 910781086
14 Crenshaw M A Thesoluble matrix from Mercenaria mercenaria shellBiomineralization 1972 6611
15 Marin F Amons R Guichard N et al.Caspartin and calprismin, two proteins of the shellcalcitic prism of the mediterranean fan mussel Pinna nobilisThe Journal ofBiological Chemistry 2005 2803389533908.
doi:10.1074/jbc.M506526200
16 Takakura D Norizuki M Ishikawa F et al.Isolation and characterization of the N-linked oligosaccharides in nacrein from Pinctada fucataMarine Biotechnology (in press)
17 Laemmli U K Cleavageof structural proteins during the assembly of the head of BacteriophageT4Nature 1970 227680685.
doi:10.1038/227680a0
18 Itoh S Kawasaki N Hashii N et al.N-linked oligosaccharideanalysis of rat brain Thy-1 by liquid chromatography with graphitizedcarbon column/ion trap-Fourier transform ion cyclotron resonance massspectrometry in positive and negative ion modesJournal of Chromatography A 2006 1103296306.
doi:10.1016/j.chroma.2005.11.043
19 Kawasaki N Itoh S Ohta M et al.Microanalysis of N-linked oligosaccharides in a glycoprotein by capillary liquid chromatography/massspectrometry and liquid chromatography/tandem mass spectrometryAnalytical Biochemistry 2003 3161522.
doi:10.1016/S0003‐2697(03)00031‐9
20 Dauphin Y Comparisonof the soluble matrices of the calcitic prismatic layer of Pinna nobilis (Mollusca, Bivalvia, Pteriomorpha)Comparative Biochemistry and Physiology Part A: Molecular &Integrative Physiology 2002 132(3)577590.
doi:10.1016/S1095‐6433(02)00099‐5
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