Please wait a minute...
Frontiers of Materials Science

ISSN 2095-025X

ISSN 2095-0268(Online)

CN 11-5985/TB

Postal Subscription Code 80-974

2018 Impact Factor: 1.701

Front. Mater. Sci.    2008, Vol. 2 Issue (2) : 221-227    https://doi.org/10.1007/s11706-008-0037-1
Improvements of anticoagulant activities of silk fibroin films with fucoidan
GAO Zhen1, WANG Song1, ZHU He-sun2, ZHAO Dong-xu3, XU Jia-chao4
1.Research Center of Material Science, Beijing Institute of Technology;School of Material Science and Engineering, Beijing Institute of Technology; 2.Research Center of Material Science, Beijing Institute of Technology; 3.School of Life Science and Technology, Beijing Institute of Technology; 4.Department of Food Science and Engineering, Ocean University of China
 Download: PDF(292 KB)   HTML
 Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract Fucoidan (FC), an effective anticoagulant constituent extracted from brown algae, was introduced into silk fibroin (SF) for improving its blood compatibility. The SF and SF/FC blend films were characterized by attenuated total reflectance Fourier-transform infrared (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and dynamic contact angle determinator (CA). The in vitro anticoagulant activities of the films were evaluated by activated partial thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT) measurements. The endothelial cell attachment and proliferation viability on the film were assessed by micropipette aspiration technique and MTT assay, respectively. The testing results indicated that the introduction of FC increased the roughness, hydrophilicity and sulfate component of the film surface without impeding the formation of ?-sheet conformation in SF. More important, FC brought excellent anticoagulant activity and better endothelial cell affinity to SF. The SF/FC blend film was hopeful to be used as blood-contacting biomaterials.
Issue Date: 05 June 2008
 Cite this article:   
WANG Song,GAO Zhen,ZHU He-sun, et al. Improvements of anticoagulant activities of silk fibroin films with fucoidan[J]. Front. Mater. Sci., 2008, 2(2): 221-227.
 URL:  
https://academic.hep.com.cn/foms/EN/10.1007/s11706-008-0037-1
https://academic.hep.com.cn/foms/EN/Y2008/V2/I2/221
1 Vepari C Kaplan D L Silk as a biomaterialProgress in Polymer Science 2007 329911007.
doi:10.1016/j.progpolymsci.2007.05.013
2 Tretinnikov O N Tamada Y Influence of casting temperatureon the near-surface structure and wettability of cast silk fibroinfilmsLangmuir 2001 1774067413.
doi:10.1021/la010791y
3 Altman G H Diaz F Jakuba C et al.Silk-based biomaterialsBiomaterials 2003 24(3)401416.
doi:10.1016/S0142‐9612(02)00353‐8
4 Horan R L Antle K Collette A L et al.In vitro degradationof silk fibroinBiomaterials 2005 26(17)33853393.
doi:10.1016/j.biomaterials.2004.09.020
5 Minoura N Aiba S Gotoh Y et al.Attachment and growth of cultured fibroblast cellson silk protein matricesJournal of BiomedicalMaterial Research 1995 29(10)12151221.
doi:10.1002/jbm.820291008
6 Hu K Lv Q Cui F Z et al.Biocompatible fibroin blended films with recombinanthuman-like collagen for hepatic tissue engineeringJournal of Bioactive Compatible Polymer 2006 21(1)2337.
doi:10.1177/0883911506060455
7 Arai T Freddi G Innocenti R et al.Biodegradation of B. mori silk fibroin fibers and filmsJournalof Applied Polymer Science 2004 9123832390.
doi:10.1002/app.13393
8 Ma X L Cao C B Zhu H S The biocompatibility of silk fibroin films containing sulfonatedsilk fibroinJournal of Biomedical MaterialResearch Part B: Applied Biomaterials 2006 78(1)8996.
doi:10.1002/jbm.b.30466
9 Lv Q Cao C B Zhu H S Clotting times and tensile properties of insoluble silkfibroin films containing heparinPolymerInternational 2005 5410761081.
doi:10.1002/pi.1814
10 Feng G L Wang S Zhu H S Immobilization of heparin to silk fibroin/collagen blendfilms and in vitro antithrombogenicity assessmentJournal of Functional Materials 2005 36(1)150152 (in Chinese)
11 Kelto J G Smith J W Warkentin T E Immunoglobulin from patients with heparin-induced thrombocytopeniabinds to a complex of heparin and platelet factorBlood 1994 8332323239
12 Warkentin T E Levine M N Hirsh J Heparin-induced thrombocytopenia in patients treated withlow-molecular-weight heparin or unfractionated heparinThe New England Journal of Medicine 1995 33213301335.
doi:10.1056/NEJM199505183322003
13 Von Appen K Ivanovich P Mujais S et al.Endothelium: the next frontier in biocompatibilityArtificial Organs 1993 17(2)985995
14 Mu X N Therole of vascular endothelial cells in thrombosis caused by biomaterialsBiomedical Engineering Foreign Medical Sciences 2004 27(2)7478 (in Chinese)
15 Stanley J C Burkel W E Ford J C et al.Enhanced patency of small diameter externally supportedDacron iliofemoral grafts seeded with endothelial cellsSurgery 1982 929941005
16 Springer G F Wurzel H A Mcsneal G M Isolation of anticoagulant fractions from crude fucoidanProceedings of the Society for Experimental Biologyand Medicine 1957 94404409
17 Colliec S Fischer A M Tapon-Bretaudiere J et al.Anticogulant properties of a fucoidanfractionThrombosis Research 1991 64143154.
doi:10.1016/0049‐3848(91)90114‐C
18 Church F C Meade J B Treanor R E et al.Anticoagulant activity of fucoidanJournal of Biological Chemistry 1989 26436183623
19 Fábio R M Mariana S P Débora F et al.Antithrombin-mediated anticoagulant activity ofsulfated polysaccharidesThe Journal ofBiological Chemistry 2004 2792082420835.
doi:10.1074/jbc.M308688200
20 Lv Q Cao C B Zhang Y et al.Preparation of insoluble fibroin films without methanoltreatmentJournal of Applied Polymer Science 2005 9621682173.
doi:10.1002/app.21682
21 Gao Z Wang S Zhu H S et al.Using selected uniform cells in round shape witha micropipette to measure cell adhesion strength on silk fibroin basedmaterialsMaterials Science and Engineering:C 2007 .
doi: .
doi: 10.1016/j.msec.2007.11.003 (in press)
22 Song G B Luo Q Qin J et al.Expression of integrin β1 and its roles onadhesion between different cell cycle hepatocellular carcinoma cells(SMMC-7721) and human umbilical vein endothelial cellsColloids and Surfaces B: Biointerfaces 2004 34247252.
doi:10.1016/j.colsurfb.2004.01.009
23 Wilson D Valluzzi R Kaplan D L Conformational transitions in model silk peptidesBiophysical Journal 2000 7826902701
24 Nam J Park Y H Morphology of regenerated silkfibroin: Effects of freezing temperature, alcohol addition, and molecularweightJournal of Applied Polymer Science 2001 8130083021.
doi:10.1002/app.1751
25 Hsieh T Hsieh K Simon G P et al.Effect of cross-linking density on the physicalproperties of interpenetrating polymer networks of polyurethane and2-hydroxyethyl methacrylate-teminated polyurethaneJournal of Polymer Research 1998 5153162.
doi:10.1007/s10965‐006‐0051‐x
26 Yeh Y S Iriyama Y Matsuzawa Y et al.Blood compatibility of surfaces modified by plasmapolymerizationJournal of Biomedical MaterialsResearch 1988 22795818.
doi:10.1002/jbm.820220904
27 Lanza R P Langer R Chick W L Principles of Tissue EngineeringSan DiegoAcademic Press 2000
28 Webb K Hlady V Tresco P A Relationships among cell attachment, spreading, cytoskeletalorganization, and migration rate for anchorage dependent cells onmodel surfacesJournal of Biomedical MaterialsResearch 2000 49362368.
doi:10.1002/(SICI)1097‐4636(20000305)49:3<362::AID‐JBM9>3.0.CO;2‐S
29 Yang J C Song L H Zhou J H et al.Medical Cell EngineeringShanghaiShanghai Jiao Tong UniversityPress 2003
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed