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Frontiers of Materials Science

ISSN 2095-025X

ISSN 2095-0268(Online)

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Front Mater Sci Chin    2010, Vol. 4 Issue (4) : 359-365    https://doi.org/10.1007/s11706-010-0112-2
RESEARCH ARTICLE
Antibacterial and biological properties of silver-loaded coralline hydroxyapatite
Yu ZHANG1,2(), Qing-Shui YIN1, Hua-Fu ZHAO1, Jian LI1, Yue-Teng WEI3, Fu-Zhai CUI3, Hua-Yang HUANG1
1. Department of Orthopedic Surgery, General Hospital of Guangzhou Military Command, Guangzhou 510010, China; 2. Post-Graduate Institute, South China University of Technology, Guangzhou 510000, China; 3. Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China
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Abstract

The antibacterial and biological properties of silver-loated coralline hydroxyapatite (Ag-CHA) as a new antibacterial implant material were investigated in this study. Compared to other antibiotic and chemical bactericidal agents, Ag+ does not bring bacterial resistance to drugs and has less toxicity. The porous CHA was formed by hydrothermal exchange, then Ag+ was loated onto CHA through ion exchange and adsorption. The microstructure and composition of Ag-CHA were characterized by scanning electron microscopy (SEM), Rutherford backscattering spectrometry (RBS), and energy dispersive spectrometry (EDS). Antibacterial activity of Ag-CHA on the clinical strains of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) was evaluated by the flat plate diffusion method. The antibacterial activity of Ag-CHA was found to be correlated with the concentration of Ag+ in a dose-dependent manner, which indicated that the optimal antibacterial and biocompatible effects of Ag-CHA could be obtained with Ag+ concentrations from 5×10-5 to 1×10-4 mol/L.
Keywords silver-loated loralline hydroxyapatite (Ag-CHA)      antibacterial activity      cytotoxicity     
Corresponding Author(s): ZHANG Yu,Email:luck_2001@126.com   
Issue Date: 05 December 2010
 Cite this article:   
Yu ZHANG,Qing-Shui YIN,Hua-Fu ZHAO, et al. Antibacterial and biological properties of silver-loaded coralline hydroxyapatite[J]. Front Mater Sci Chin, 2010, 4(4): 359-365.
 URL:  
https://academic.hep.com.cn/foms/EN/10.1007/s11706-010-0112-2
https://academic.hep.com.cn/foms/EN/Y2010/V4/I4/359
Fig.1  Scanning electron microscopy (SEM) microstructures of Ag-CHA in different Ag concentration (red arrows showed the Ag particles adhered onto the coral hydroxyapatite; the same as CHA, the porosity of Ag-CHA was more than 40% and the diameters of the pores were about 150-250 μm): 1×10 mol/L; 1×10 mol/L; 5×10 mol/L; 1×10 mol/L; 8×10 mol/L; 5×10 mol/L; 1×10 mol/L; 0 mol/L; 1×10 mol/L
Fig.2  Rutherford backscattering spectrometry (RBS) analysis of Ag-CHA at different Ag concentrations (Extremely small shinning particles (shown by the red arrows) with 0.2-0.5 μm in diameter were found on the surface in high concentrations of Ag-CHA and were rarely found in low concentrations of Ag-CHA): 1×10 mol/L; 1×10 mol/L; 5×10 mol/L; 1×10 mol/L; 8×10 mol/L; 5×10 mol/L; 1×10 mol/L; 0 mol/L
ElementAtom ratio /at.%
[Ag]/(mol·L-1)=1×10-2[Ag]/(mol·L-1)=1×10-3[Ag]/(mol·L-1)=5×10-4[Ag]/(mol·L-1)=1×10-4[Ag]/(mol·L-1)=8×10-5[Ag]/(mol·L-1)=5×10-5[Ag]/(mol·L-1)=1×10-5[Ag]/(mol·L-1)=0
C19.3917.8421.2518.9416.6124.6227.8114.34
O53.9955.2153.9657.0758.2452.8659.4755.26
P7.859.319.068.189.261.341.8911.67
Ag4.552.331.961.781.520.030.03–
Ca13.7515.0413.6414.0014.3718.959.8418.73
Tab.1  Atom ratio of elemental components of Ag-CHA
Fig.3  Comparison of inhibition zones of Ag-CHA on and after 24 h (=3)
GroupDiameter of the inhibition zone /mmFP
[Ag]/(mol·L-1)=1×10-2[Ag]/(mol·L-1)=1×10-3[Ag]/(mol·L-1)=5×10-4[Ag]/(mol·L-1)=1×10-4[Ag]/(mol·L-1)=8×10-5
E. coli17.68±0.3416.24±0.78 a)14.82±1.50 a)15.13±1.7816.14±0.862.7290.090
S. aureus13.04±0.40 13.38±1.3013.05±0.6212.76±0.6612.48±0.48 0.5830.682
t-14.107-2.882-1.685-1.468-8.460
P0.0050.1020.2340.2800.014
Tab.2  Comparison of inhibition zones of Ag-CHA on and after 24 h (±, =3)
Fig.4  Cytotoxicity of 100% leaching solutions of Ag-CHA on L929 cells: blank control; CHA; 1×10 mol/L Ag-CHA; 1×10 mol/L Ag-CHA; 5×10 mol/L Ag-CHA; 1×10 mol/L Ag-CHA; 8×10 mol/L Ag-CHA; 5×10 mol/L Ag-CHA; 1×10 mol/L Ag-CHA (magnification: ×100)
Group25% SolutionGrade50% SolutionGrade100% SolutionGrade
CHA111.6%0109.9%0104.0%0
[Ag+]/(mol·L-1)=1×10-29.9%48.5%46.2%4
[Ag+]/(mol·L-1)=1×10-3120.3%013.2%46.6%4
[Ag+]/(mol·L-1)=5×10-4124.5%012.2%45.4%4
[Ag+]/(mol·L-1)=1×10-4131.6%017.2%46.4%4
[Ag+]/(mol·L-1)=8×10-5100%0105.6%094.2%1
[Ag+]/(mol·L-1)=5×10-5120%0127.9%0121.4%0
[Ag+]/(mol·L-1)=1×10-5111.6%0109.9%0104%0
Tab.3  Evaluation of cytotoxicity of Ag-CHA on L929 cells with MTT method (=3)
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