Electrospinning of curcumin loaded chitosan/poly (lactic acid) nanofilm and evaluation of its medicinal characteristics

doi:10.1007/s11706-013-0222-8

Front Mater Sci

2013, Vol. 7

Issue (4) : 350-361 https://doi.org/10.1007/s11706-013-0222-8

RESEARCH ARTICLE

Electrospinning of curcumin loaded chitosan/poly (lactic acid) nanofilm and evaluation of its medicinal characteristics

Bhaarathi DHURAI¹(

), Nachimuthu SARASWATHY², Ramasamy MAHESWARAN¹, Ponnusamy SETHUPATHI¹, Palanisamy VANITHA¹, Sukumar VIGNESHWARAN¹, Venugopal RAMESHBABU¹

1. Department of Textile Technology, Kumaraguru College of Technology, Coimbatore 641049, Tamilnadu, India; 2. Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641049, Tamilnadu, India

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Abstract

The curcumin loaded chitosan/poly (lactic acid) (PLA) nanofibers were produced using electrospinning. Box-Behnken experimental design was used for the optimization of variables (–1, 0, +1 coded level) like chitosan/PLA strength (% w/v), curcumin strength (% w/v) and applied voltage (kV) to obtain uniform fiber diameter. The morphology of nanofibers was shown by SEM. Molecular interactions and the presence of each chemical compound of curcumin loaded chitosan/PLA fibers were characterized by FTIR and EDX analysis. Antioxidant, drug release and in vitro cytotoxicity tests were performed to evaluate the suitability of nanofibers that would be used for wound healing. In vivo wound healing studies on excision and incision wounds created on rat model showed significant reduction of wound area when compared to untreated. The better healing efficiency can be attributed to the presence of curcumin and chitosan.

Keywords Box-Behnken chitosan curcumin electrospinning poly (lactic acid) (PLA)

Corresponding Author(s): DHURAI Bhaarathi,Email:bhaarathi_dhurai@yahoo.com

Issue Date: 05 December 2013

Cite this article:

Bhaarathi DHURAI,Nachimuthu SARASWATHY,Ramasamy MAHESWARAN, et al. Electrospinning of curcumin loaded chitosan/poly (lactic acid) nanofilm and evaluation of its medicinal characteristics[J]. Front Mater Sci, 2013, 7(4): 350-361.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-013-0222-8
https://academic.hep.com.cn/foms/EN/Y2013/V7/I4/350

Tab.1 Process parameters at different coded levels

Tab.2 Box-Behnken experimental design

Fig.1 Contour plots of chitosan/PLA strength and curcumin strength on the nanofiber diameter, chitosan/PLA strength and applied voltage on the nanofiber diameter, and curcumin strength and applied voltage on the nanofiber diameter.

Fig.2 Optimization plot.

Fig.3 SEM image of optimized sample.

Fig.4 Element analysis of optimized sample.

Tab.3 Weight percent of each element

Fig.5 FTIR analysis of curcumin powder, chitosan/PLA nanofilm and chitosan/PLA nanofilm loaded with curcumin.

Tab.4 Antioxidant activity of curcumin loaded chitosan/PLA nanofibers ( = 3)

Fig.6 Drug release of nanofilm.

Tab.5 Cytotoxicity grading of samples

Fig.7 Excision wound on rat model.

Tab.6 Percentage reduction on wound size (excision wound rat model)

Tab.7 Effect of sample in incision wound rat model

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