Synthesis, characterization and biological evaluation of poly [LA-co-(Glc-alt-Lys)] for nerve regeneration scaffold

doi:10.1007/s11706-014-0239-7

Front. Mater. Sci.

2014, Vol. 8

Issue (1) : 95-101 https://doi.org/10.1007/s11706-014-0239-7

RESEARCH ARTICLE

Synthesis, characterization and biological evaluation of poly [LA-co-(Glc-alt-Lys)] for nerve regeneration scaffold

Yi-Xia YIN,Ji-Ling YI,Li-Juan XIE,Qiong-Jiao YAN,Hong-Lian DAI,Shi-Pu LI(

)

Biomedical Materials and Engineering Research Center, Wuhan University of Technology, Wuhan 430070, China

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Abstract

A novel nerve repairing material poly [LA-co-(Glc-alt-Lys)] (PLGL) was synthesized. The viability and growth of Schwann cells (SCs) co-cultured with poly (D, L-lactic acid) (PDLLA) films (control group) and PLGL films were evaluated by MTT assay and SEM observation. Then, contact angle measurement, histological assessment and enzyme-linked immunosorbent assay (ELISA) testing on inflammatory-related cytokines such as IL-10 and TGF-β1 were performed. The results showed that, compared with PDLLA, PLGL films possesses better hydrophilicity, biocompatibility, degradation property and less inflammatory reaction. The present study indicated that PLGL scaffolds would meet the requirements of artificial nerve scaffold and have a potential application in the fields of nerve regeneration.

Keywords Schwann cell (SC) poly [LA-co-(Glc-alt-Lys)] (PLGL) implantation inflammatory factor biocompatibility

Corresponding Author(s): Shi-Pu LI

Issue Date: 24 June 2014

Cite this article:

Yi-Xia YIN,Ji-Ling YI,Li-Juan XIE, et al. Synthesis, characterization and biological evaluation of poly [LA-co-(Glc-alt-Lys)] for nerve regeneration scaffold[J]. Front. Mater. Sci., 2014, 8(1): 95-101.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-014-0239-7
https://academic.hep.com.cn/foms/EN/Y2014/V8/I1/95

Fig.1 The morphology of SCs (a) identified by S-100 immunocytochemistry and (b) observed under optical inversion microscope after cultured for 4 d.

Fig.2 ¹H NMR spectrum of PLGL.

Tab.1 Contact angle to water of PDLLA and PLGL films

Fig.3 SCs viability on films measured by MTT assay.

Fig.4 SEM images of SCs on (a) PDLLA film and (b) PLGL film.

Fig.5 (a) Macroscopic appearance of the sciatic nerve implanting in PLGL conduit. (b) HE staining of subcutaneous tissue after PDLLA implantation. (c) HE staining of subcutaneous tissue after PLGL implantation.

Fig.6 The concentrations of (a) TGF-β1 and (b) IL-10 after scaffold implantation at 1 and 5 weeks (N = 5; #: compared with PLGL, p < 0.05;*: compared with normal, p < 0.05).

Tab.2

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