Preparation and properties of calcium sulfate bone cement incorporated with silk fibroin and Sema3A-loaded chitosan microspheres

doi:10.1007/s11706-015-0278-8

Front. Mater. Sci.

2015, Vol. 9

Issue (1) : 51-65 https://doi.org/10.1007/s11706-015-0278-8

RESEARCH ARTICLE

Preparation and properties of calcium sulfate bone cement incorporated with silk fibroin and Sema3A-loaded chitosan microspheres

Peng WANG,Bin PI,Jin-Ning WANG,Xue-Song ZHU(

),Hui-Lin YANG(

)

Department of Orthopedic Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, China

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Abstract

To search for new bioactive materials which can be used as the substitute of bone repairing and drug carriers, Sema3A-loaded chitosan microspheres (SLCM) and silk fibroin (SF) were mixed with calcium sulfate cement (CSC). SEM, particle size analysis and swelling rate determination were performed to study properties of the microspheres. The drug loading, encapsulation efficiency and drug release rate were determined by ELISA. Microspheres with different SLCM weight contents (0.5%, 1% and 5%) were prepared to determine which one has the strongest mechanical properties and the appropriate setting time. It was revealed that CSC/SF/0.5SLCM has satisfactory mechanical properties, and its in vitro biocompatibility was assessed by MTS. Chitosan microspheres (5--18 μm) were globular, the surface was smooth, and the swelling rate is (77.02±5.57)%. With this formula, the setting time was increased with the addition of SLCM in CSC/SF, and the cumulative drug release rate is 44.62% in 28 d. XRD results demonstrate that the main component is calcium sulfate. Also it was found that CSC/SF/0.5SLCM supports the growth of MC3T3 cells. Thus the preparation of CSC/SF/0.5SLCM was reliable, and the products had good structures, physical properties and biocompati-bility, appearing to be a promising bone substitute material.

Keywords Sema3A chitosan microsphere sustained release silk fibroin (SF) bone cement

Corresponding Author(s): Xue-Song ZHU,Hui-Lin YANG

Online First Date: 04 February 2015 Issue Date: 02 March 2015

Cite this article:

Peng WANG,Bin PI,Jin-Ning WANG, et al. Preparation and properties of calcium sulfate bone cement incorporated with silk fibroin and Sema3A-loaded chitosan microspheres[J]. Front. Mater. Sci., 2015, 9(1): 51-65.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-015-0278-8
https://academic.hep.com.cn/foms/EN/Y2015/V9/I1/51

Fig.1 The particle size distribution of SLCM soaked in double distilled water.

Fig.2 Morphology of chitosan microspheres by (a) light microscopy and (b) electron microscopy.

Fig.3 Compressive strength of CSC/SF/SLCM with different proportions of microspheres.

Fig.4 Setting time of CSC/SF/SLCM with different proportions of microspheres.

Fig.5 XRD patterns of different types of bone cement: calcium sulfate powder, CSC, CSC/SF and CSC/SF/0.5SLCM.

Fig.6 SEM images of bone cements: (a) CSC; (b) CSC/SF; (c) CSC/SF/0.5SLCM; (d) CSC immersed in PBS for two months; (e) CSC/SF immersed in PBS for two months; (f) CSC/SF/0.5SLCM immersed in PBS for two months.

Fig.7 Sema3A cumulative release rate of SLCM and CSC/SF/0.5SLCM in 28 d.

Fig.8 The degradation of CSC, CSC/SF and CSC/SF/0.5SLCM composite cement soaked in PBS for 8 weeks.

Fig.9 MTS assay of MC3T3-E1 cells cultured in α-MEM supplemented with CSC, CSC/SF and CSC/SF/0.5SLCM powder extracts.

Fig.10 SEM image of MC3T3 cells seeded onto the surface of CSC/SF/0.5SLCM.

Fig.11 MicroCT photographs of new bone formation in the defect area in 8 weeks of implantation.

Fig.12 Amounts of (a) new bone formation (BV) and (b) residual material (MV) relative to the total volume in the defect area in 8 weeks of implantation.

Fig.13 Histological analyses of the explants in 2, 4, 6 and 8 weeks after implantation.

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