Characterization of decellularized scaffold derived from porcine meniscus for tissue engineering applications

doi:10.1007/s11706-016-0335-y

Front. Mater. Sci.

2016, Vol. 10

Issue (2) : 101-112 https://doi.org/10.1007/s11706-016-0335-y

RESEARCH ARTICLE

Characterization of decellularized scaffold derived from porcine meniscus for tissue engineering applications

Shuang GAO¹,Zhiguo YUAN²,Tingfei XI^1,^*(

),Xiaojuan WEI^1,³,Quanyi GUO²

1. Center for Biomaterial and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
2. Key Laboratory of PLA, Institute of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, China
3. Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China

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Abstract

Menisci are fundamental fibrocartilaginous organs in knee joints. The injury in meniscus can impair normal knee function and predisposes patients to osteoarthritis. This study prepared decellularized meniscus scaffolds using a 1% (w/w) sodium dodecyl sulfate solution and sufficient rinsing steps. Complete cell removal was verified by hematoxylin and eosin staining and DNA content assay. Decellularized menisci had accordant tension properties to intact ones, but with declined compression properties. This occurred because the collagen fiber was not damaged but glycosaminoglycans was significantly lost during the decellularization process, which was confirmed by biochemical assay and histology staining. In vitro cytotoxicity assay demonstrated that decellularized meniscus scaffolds have no toxicity on L929 murine fibroblasts and porcine chondrocytes. Further experiment showed that porcine chondrocytes could adhere and proliferate on the scaffold surface, and some cells even could infiltrate into the scaffold. All results showed the potential of this decellularized meniscus to be the scaffolds in tissue engineering.

Keywords meniscus scaffold decellularization biomechanical cytotoxicity tissue engineering

Corresponding Author(s): Tingfei XI

Online First Date: 06 April 2016 Issue Date: 11 May 2016

Cite this article:

Shuang GAO,Zhiguo YUAN,Tingfei XI, et al. Characterization of decellularized scaffold derived from porcine meniscus for tissue engineering applications[J]. Front. Mater. Sci., 2016, 10(2): 101-112.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-016-0335-y
https://academic.hep.com.cn/foms/EN/Y2016/V10/I2/101

Fig.1 SEM images of (A) intact and (B) decellularized menisci. (C) Macroscopic features of intact and decellularized menisci.

Fig.2 Histology staining of intact and decellularized menisci. H&E staining of (A) intact and (B) decellularized menisci. Sirius red staining of (C) intact meniscus and (C₁) viewed in polarized light field; (D) decellularized meniscus and (D₁) viewed in polarized light field. Alcian blue staining of (E) intact and (F) decellularized menisci.

Tab.1 DNA, hydroxyproline and sGAG content in intact and decellularized menisci

Tab.2 Mechanical properties in intact and decellularized menisci

Fig.3 Cytotoxicity to L929 cells and PCs by the extract and contact methods in vitro (* p<0.05, by ANOVA).

Fig.4 (A)(B)(C) Live/dead staining of PCs seeded on decellularized meniscus scaffolds. (D)(E)(F) SEM images of PCs seeded on decellularized meniscus scaffolds.

Fig.5 (A) Cytoactivity of PCs seeded on scaffolds. (B) H&E staining of PCs cultured on scaffolds at 7 d.

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