The comparison of the Wnt signaling pathway inhibitor delivered electrospun nanoyarn fabricated with two methods for the application of urethroplasty

doi:10.1007/s11706-016-0359-3

Front. Mater. Sci.

2016, Vol. 10

Issue (4) : 346-357 https://doi.org/10.1007/s11706-016-0359-3

RESEARCH ARTICLE

The comparison of the Wnt signaling pathway inhibitor delivered electrospun nanoyarn fabricated with two methods for the application of urethroplasty

Xuran GUO¹,Kaile ZHANG²,Mohamed EL-AASSAR^1,³,Nanping WANG⁴,Hany EL-HAMSHARY^5,⁶,Mohamed EL-NEWEHY^5,⁶,Qiang FU²(

),Xiumei MO^1,⁷(

)

¹. State Key Lab for Modification of Chemical Fibers & Polymer Materials, College of Chemistry & Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China
². Department of Urology, Affiliated Sixth People’s Hospital, Shanghai Jiaotong University, Shanghai 200030, China
³. Polymer Materials Research Department, Advanced Technology and New Material Institute, City?of?Scientific?Research?and?Technological?Applications?(SRTA-City),?New?Borg?El-Arab?City, Alexandria 21934,?Egypt
⁴. Shanghai Aquatic Product Research Institute,?Shanghai 200030,?China
⁵. Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
⁶. Department of Chemistry, Faculty of Science,?Tanta?University,?Tanta?31527,?Egypt
⁷. Shandong International Biotechnology Park Development Co., Ltd.,?Shanghai 200030,?China

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Abstract

Urethral strictures were common disease caused by over-expression of extracellular matrix from fibroblast. In this study, we compare two nanoyarn scaffolds for improving fibroblasts infiltration without inhibition the?over-expression of extracellular matrix. Collagen/poly(L-lactide-co-caprolactone)?(Col/P(LLA-CL)) nanoyarn scaffolds were prepared by conjugated electrospinning and dynamic liquid electrospinning, respectively. In addition, co-axial electrospinning technique was combined with the nanoyarn fabrication process to produce nanoyarn scaffolds loading?Wnt?signaling pathway inhibitor. The mechanical properties of the scaffolds were examined and morphology was observed by SEM. Cell morphology, proliferation and infiltration on the scaffolds were investigated by SEM, MTT assay and H&E staining, respectively. The release profiles?of different scaffolds were?determined using?HPLC. The results indicated that cells showed an organized morphology along the?nanoyarns and considerable infiltration?into the nanoyarn scaffolds prepared by dynamic liquid electrospinning (DLY). It was also observed that the DLY significantly facilitate cell proliferation. The D-DLY could facilitate the infiltration of the fibroblasts and could be a promising scaffold for the treatment of urethra stricture while it may inhibit the collagen production.

Keywords biomaterials nanoyarn electrospinning inhibitor

Corresponding Author(s): Qiang FU,Xiumei MO

Online First Date: 14 November 2016 Issue Date: 24 November 2016

Cite this article:

Xuran GUO,Kaile ZHANG,Mohamed EL-AASSAR, et al. The comparison of the Wnt signaling pathway inhibitor delivered electrospun nanoyarn fabricated with two methods for the application of urethroplasty[J]. Front. Mater. Sci., 2016, 10(4): 346-357.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-016-0359-3
https://academic.hep.com.cn/foms/EN/Y2016/V10/I4/346

Fig.1 The chemical structure of ICG-001.

Fig.2 The schematic diagrams of (a) the fabrication of nanoyarn with the dynamic liquid system and (b) the co-axial electrospinning device.

Fig.3 The schematic diagram of the conjugated electrospinning.

Fig.4 SEM images and characteristics of different scaffolds, and their fiber diameter distributions of scaffolds: (a) CY; (b) DLY; (c) NF; (d) D-CY; (e) D-DLY; (f) D-NF.

Fig.5 The TEM image of electrospun Col/P(LLA-CL) loading ICG-001. Red arrows indicate ICG-001.

Fig.6 Mechanical properties of different scaffolds (* p<0.05; ** p<0.01): (a) tensile stress–strain curves; (b) tensile strength at break; (c) strain at break.

Fig.7 The porosity and the pore diameter of different scaffolds.

Fig.8 The images of water droplets on the surface of different scaffolds and water contact angle values.

Fig.9 The cumulative release of the ICG-001 delivered in different scaffolds in 8 weeks in vitro.

Fig.10 (a) The gross morphology and (b) the immunofluorescence of fibroblasts stained with vimentin antibody.

Fig.11 SEM images of cells on scaffolds on day 7 after culture: (a) D-DLY, (d) DLY, (b) D-CY, (e) CY, (c) D-NF and (f) NF.

Fig.12 The relative absorption of MTT assay for fibroblast cells on different scaffolds (DLY, D-DLY, CY, D-CY, NF and D-NF) and cover slips for control on 1, 3, 5, 7 d.

Fig.13 The immunofluorescence of collagen type 1 expression from fibroblasts: (a) fibroblasts without treatment; (b) fibroblasts treated with released medium from D-DLY; (c) fibroblasts treated with released medium from D-CY; (d) fibroblasts treated with released medium from D-NF.

Fig.14 The infiltration of cells in the scaffolds. Cells seeded (a)(c) CY and (b)(d) D-CY at 2 and 3 weeks with different magnification. Cells seeded (e)(g) DLY and (f)(h) D-DLY at 2 and 3 weeks with different magnification.

Fig.1 Fig. S1&chsp;The H&E staining image of fibroblast seeded on nanofiber scaffold (NF).

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