Injectable hydrogel wound dressing based on strontium ion cross-linked starch

doi:10.1007/s11706-020-0508-6

Front. Mater. Sci.

2020, Vol. 14

Issue (2) : 232-241 https://doi.org/10.1007/s11706-020-0508-6

RESEARCH ARTICLE

Injectable hydrogel wound dressing based on strontium ion cross-linked starch

Yuxuan MAO¹, Mingming PAN², Huilin YANG², Xiao LIN^1,²(

), Lei YANG^1,^2,³(

)

¹. College of Chemistry, Chemical Engineering and Materials Science, Orthopaedic Institute, Soochow University, Suzhou 215006, China
². Department of Orthopaedics, The First Affiliated Hospital, Soochow University, Suzhou 215006, China
³. Center for Health Science and Engineering, School of Materials Science and Engineering, Hebei University of Technology, Tianjin 300131, China

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Abstract

Severe skin wounds cause great problems and sufferings to patients. In this study, an injectable wound dressing based on strontium ion cross-linked starch hydrogel (SSH) was developed and evaluated. The good inject-ability of SSH made it easy to be delivered onto the wound surface. The good tissue adhesiveness of SSH ensured a firm protection of the wound. Besides, SSH supported the proliferation of NIH/3T3 fibroblasts and facilitated the migration of human umbilical vein endothelial cells (HUVECs). Importantly, SSH exhibited strong antibacterial effects on Staphylococcus aureus (S. aureus), which could prevent wound infection. These results demonstrate that SSH is a promising wound dressing material for promoting wound healing.

Keywords wound dressing injectable antibacterial gel-point adhesive hydrogel (GPAH)

Corresponding Author(s): Xiao LIN,Lei YANG

Online First Date: 14 May 2020 Issue Date: 27 May 2020

Cite this article:

Yuxuan MAO,Mingming PAN,Huilin YANG, et al. Injectable hydrogel wound dressing based on strontium ion cross-linked starch[J]. Front. Mater. Sci., 2020, 14(2): 232-241.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-020-0508-6
https://academic.hep.com.cn/foms/EN/Y2020/V14/I2/232

Fig.1 Characterization of SSH: (a) photographs of SSH; (b) FTIR spectrum of SSH; (c) SEM image of the SSH surface; (d) SEM image of the cross section of SSH; (e) variation of the water content of SSH during aging at 25 °C and 60% relative humidity.

Fig.2 Rheology tests of SSH: (a) G', G'' and G''/G' of starch hydrogel on frequency sweep; (b) G', G'' and G''/G' of SSH on frequency sweep; (c) G', G'' and G''/G' of SSH on strain sweep; (d) the rheological properties of SSH when alternate step strain switched from 1% to 500%; (e) photograph of the SSH injection performance.

Fig.3 Adhesion characterization of SSH: (a) photographs of SSH adhered onto weight and biological tissues; (b) the relationship between stress and displacement during the adhesiveness test; (c) material/porcine skin interfacial strength; (d) material/porcine skin interfacial toughness.

*

p<0.05.

Fig.4 Cell experiment of SSH: (a) viability of NIH/3T3 fibroblasts when cultured with the SSH extracts for 1 and 3?d versus a control group (viability set as 100%); (b) fluorescence micrographs of NIH/3T3 fibroblasts after culture with SSH extracts or regular cell culture media for 1 and 3?d (Green: living cells; Red: dead cells; Arrow pointing to dead cells); (c) microscopic photographs of HUVECs migration when cultured with SSH extracts for 0, 12, 24, 36, and 48?h.

Fig.5 Antibacterial capability of SSH: (a) number of S. aureus after culture with SSH extracts or LB (control) for 12 h; (b) antibacterial activity against S. aureus of SSH; (c) photographs of S. aureus on agar plates after diluted bacteria cultured with SSH extracts or LB for 12 h (×10: the bacterial solution after 12 h of culture was diluted 10 times; ×10000: the bacterial solution after 12 h of culture was diluted 10000 times).

*

p<0.05.

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