Antimicrobial power of biosynthesized Ag nanoparticles using refined <i>Ginkgo biloba</i> leaf extracts

doi:10.1007/s11706-022-0594-8

Front. Mater. Sci.

2022, Vol. 16

Issue (2) : 220594 https://doi.org/10.1007/s11706-022-0594-8

RESEARCH ARTICLE

Antimicrobial power of biosynthesized Ag nanoparticles using refined Ginkgo biloba leaf extracts

Wenbo ZHUANG¹, Dafeng HU¹, Xudong ZHANG¹, Kai XIONG¹, Xiao DING², Jian LU³, Yong MAO¹, Peng YANG¹(

), Chao LIU³(

), Yanfen WAN¹(

)

¹. National Center for International Research on Photoelectric and Energy Materials, Advanced Computing Center, Materials Genome Institute, School of Materials and Energy, Yunnan University, Kunming 650091, China
². Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
³. Department of Nuclear Medicine, Yunnan Cancer Hospital & The Third Affiliated Hospital of Kunming Medical University, Kunming 650118, China

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Abstract

Silver nanoparticles (Ag NPs), relative to existing antibacterial agents, are more effective, less toxic and more economical, and have shown enormous potential for the nanomedicine application. In this work, we report a ‘green’ method for the rapid and efficient synthesis of Ag NPs using Ginkgo biloba extracts as reducing agent and capping agent. The properties of Ag NPs against fungi and bacteria were investigated. The results showed that the Ginkgo biloba extracts are crucial for the preparation of uniform and monodispersed Ag NPs. The prepared Ag NPs exhibited remarkable antibacterial activities. The minimum inhibitory concentrations of Ag NPs for Escherichia coli and Pseudomonas aeruginosa were 0.044 and 0.088 μg·mL⁻¹, respectively. Moreover, Ag NPs exhibited excellent bactericidal performance against MDR-Pseudomonas aeruginosa. It was found that the effect of the antibacterial activity of Ag NPs on Escherichia coli and Staphylococcus aureus was tightly related to the reactive oxygen species accumulation. This research provides guidelines for the efficient green synthesis of Ag NPs and its antibacterial applications.

Keywords Ginkgo biloba extract silver nanoparticle green synthesis antibacterial application

Corresponding Author(s): Peng YANG,Chao LIU,Yanfen WAN

About author:

Miaojie Yang and Mahmood Brobbey Oppong contributed equally to this work.

Issue Date: 09 May 2022

Cite this article:

Wenbo ZHUANG,Dafeng HU,Xudong ZHANG, et al. Antimicrobial power of biosynthesized Ag nanoparticles using refined Ginkgo biloba leaf extracts[J]. Front. Mater. Sci., 2022, 16(2): 220594.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-022-0594-8
https://academic.hep.com.cn/foms/EN/Y2022/V16/I2/220594

Fig.1 TEM images of Ag NPs synthesized under different synthesis conditions (Ginkgo biloba extract (R), reaction time (t) and reaction temperature (θ)): (a)(b)(c) t = 30 min, θ = 40 °C, R = 0.3, 0.6 and 0.9; (d)(e)(f) R = 0.6, θ = 40 °C, t = 5, 30 and 120 min; (g)(h)(i) R = 0.6, t = 120 min, θ = 40, 60 and 80 °C.

Fig.2 (a) UV?vis absorption spectrum of Ginkgo biloba extracts. (b) FTIR spectra of Ginkgo biloba extracts (black line) and Ag NPs (red line). (c) Zeta potential spectrum of Ag NPs. (d) UV?vis absorption spectra of the Ag NP solution changing with different reaction durations (other conditions remain constant: R = 0.6, θ = 40 °C), and the top right illustration shows the Ag NP solutions with different reaction durations of 5, 30 and 120 min, respectively, from left to right.

Fig.3 The antimicrobial ability of Ag NPs: (a) the MIC of Ag NPs for Candida albicans; (b) the MIC of Ag NPs for Propionibacterium acnes; (c) the MIC of Ag NPs for Staphylococcus aureus; (d) the MIC of Ag NPs for E. coli; (e) the MIC of Ag NPs for Pseudomonas aeruginosa; (f) the MIC of Ag NPs for MDR-Pseudomonas aeruginosa (the inset is a partially enlarged view of the MIC of Ag NPs). Commercial antibiotics such as penicillin G, ampicillin and gentamicin, tetracycline, fusidine and nystatin were used as positive control or negative control according to their mechanism of actions.

Fig.4 TEM images of E. coli exposed to Ag NPs: (a) E. coli without Ag NPs; (b) E. coli exposed to Ag NPs for 2 min; (c) E. coli exposed to Ag NPs for 30 min.

Fig.5 Induced ROS levels in E. coli (upper panels) and Staphylococcus aureus (lower panels) detected by DCFDA: (a)(d) DMSO treatment was used as negative control; (b)(e) H₂O₂ treatment was used as positive control; (c)(f) Ag NPs treatment was used as experimental.

Fig.6 (a) E. coli of ROS levels induced by Ag NPs. (b) Staphylococcus aureus of ROS levels induced by Ag NPs. (c)(d)(e) Ag NPs promote the cellular oxidation process in E. coli with expressions of dmpI, narJ and narK, respectively (data were presented as mean ± SD, n = 3).

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[1]	Cheng-Zhen WEI,Hai-Feng MA,Feng GAO. Green synthesis of metal/C and metal oxide/C films by using natural membrane as support[J]. Front. Mater. Sci., 2014, 8(2): 150-156.

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