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Protein & Cell

ISSN 1674-800X

ISSN 1674-8018(Online)

CN 11-5886/Q

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Prot Cell    2012, Vol. 3 Issue (4) : 311-320    https://doi.org/10.1007/s13238-012-2027-4      PMID: 22549588
RESEARCH ARTICLE
Bisindoylmaleimide I enhances osteogenic differentiation
Fangfang Zhou1,2, Huizhe Huang2(), Long Zhang1()
1. Department of Molecular Cell Biology and Centre for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands; 2. Faculty of Basic Medical Sciences, Chongqing Medical University Medical College, Chongqing 071007, China
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Abstract

The Wnt/β-catenin and bone morphogenetic proteins (BMPs) pathways play important roles in controlling osteogenesis. Using a cell-based kinase inhibitor screening assay, we identified the compound bisindoylmaleimide I (BIM) as a potent agonist of the cytosolic β-catenin accumulation in preosteoblast cells. Through suppressing glycogen synthase kinase 3β enzyme activities, BIM upregulated β-catenin responsive transcription and extended duration of BMP initiated signal. Functional analysis revealed that BIM promoted osteoblast differentiation and bone formation. The treatment of human mesenchymal stem cells with BIM promoted osteoblastogenesis. Our findings provide a new strategy to regulate mesenchymal stem cell differentiation by integration of the cellular signaling pathways.
Keywords bisindoylmaleimide I      Wnt/β-catenin      glycogen synthase kinase 3β      bone morphogenetic protein      human mesenchymal stem cells (hMSCs)      osteogenesis     
Corresponding Author(s): Huang Huizhe,Email:devbiology@cqmu.edu.cn; Zhang Long,Email:L.Zhang@lumc.nl   
Issue Date: 01 April 2012
 Cite this article:   
Fangfang Zhou,Huizhe Huang,Long Zhang. Bisindoylmaleimide I enhances osteogenic differentiation[J]. Prot Cell, 2012, 3(4): 311-320.
 URL:  
https://academic.hep.com.cn/pac/EN/10.1007/s13238-012-2027-4
https://academic.hep.com.cn/pac/EN/Y2012/V3/I4/311
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