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ISSN 1674-8018(Online)

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Prot Cell    2012, Vol. 3 Issue (11) : 834-845    https://doi.org/10.1007/s13238-012-2078-6      PMID: 23073833
REVIEW
Overcoming barriers to the clinical utilization of iPSCs: reprogramming efficiency, safety and quality
Suying Cao1, Kyle Loh2, Yangli Pei1, Wei Zhang1, Jianyong Han1()
1. State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China; 2. Stem Cell and Developmental Biology, Genome Institute of Singapore, 138672, Singapore
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Abstract

Differentiated cells can be reprogrammed into pluripotent stem cells, known as “induced pluripotent stem cells” (iPSCs), through the overexpression of defined transcription factors. The creation of iPSC lines has opened new avenues for patient-specific cell replacement therapies for regenerative medicine. However, the clinical utilization of iPSCs is largely impeded by two limitations. The first limitation is the low efficiency of iPSCs generation from differentiated cells. The second limitation is that many iPSC lines are not authentically pluripotent, as many cell lines inefficiently differentiate into differentiated cell types when they are tested for their ability to complement embryonic development. Thus, the “quality” of iPSCs must be increased if they are to be differentiated into specialized cell types for cell replacement therapies. Overcoming these two limitations is paramount to facilitate the widespread employment of iPSCs for therapeutic purposes. Here, we summarize recent progress made in strategies enabling the efficient production of high-quality iPSCs, including choice of reprogramming factors, choice of target cell type, and strategies to improve iPSC quality.
Keywords iPSCs      reprogramming      embryonic stem cells      efficiency and quality     
Corresponding Author(s): Han Jianyong,Email:hanjy@cau.edu.cn   
Issue Date: 01 November 2012
 Cite this article:   
Suying Cao,Kyle Loh,Yangli Pei, et al. Overcoming barriers to the clinical utilization of iPSCs: reprogramming efficiency, safety and quality[J]. Prot Cell, 2012, 3(11): 834-845.
 URL:  
https://academic.hep.com.cn/pac/EN/10.1007/s13238-012-2078-6
https://academic.hep.com.cn/pac/EN/Y2012/V3/I11/834
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