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Protein & Cell

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ISSN 1674-8018(Online)

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Prot Cell    2012, Vol. 3 Issue (9) : 641-647    https://doi.org/10.1007/s13238-012-2079-5      PMID: 22926765
MINI-REVIEW
Mass spectrometry based proteomics, background, status and future needs
Peter Roepstorff()
Department of Biochemistry and Molecular Biology, University of Southern Denmark Campusvej 55, DK 5230 Odense M, Denmark
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Abstract

An overview of the background for proteomics and a description of the present state of art are given with a description of the main strategies in proteomics. The advantages and limitations of the two major strategies, 2D-gel based and LC-MS based, are discussed and a combination for the two, CeLC-MS is described. A number of challenging problems which have been solved using different proteomics strategies including the advantage of organell enrichment or modifications specific peptide isolation to get deeper into the proteome are described. Finally the present status and future needs discussed.
Keywords proteomics      2D-PAGE      LC-MS      isoforms      phosphorylation     
Corresponding Author(s): Roepstorff Peter,Email:roe@bmb.sdu.dk   
Issue Date: 01 September 2012
 Cite this article:   
Peter Roepstorff. Mass spectrometry based proteomics, background, status and future needs[J]. Prot Cell, 2012, 3(9): 641-647.
 URL:  
https://academic.hep.com.cn/pac/EN/10.1007/s13238-012-2079-5
https://academic.hep.com.cn/pac/EN/Y2012/V3/I9/641
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