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Vitamin C induces periodontal ligament progenitor cell differentiation via activation of ERK pathway mediated by PELP1 |
Yan Yan1,3, Wenfeng Zeng2, Shujun Song4, Fayun Zhang2, Wenxi He1, Wei Liang2( ), Zhongying Niu1,3() |
| 1. Department of Endodontics ,School of Stomatology, Fourth Military Medical University, Xi’an, 710032, China; 2. Protein and Peptide Pharmaceutical Laboratory, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; 3. Treatment Center of Oral Diseases, the 306th Hospital of People’s Liberation Army, Beijing 100101, China; 4. Department of Pathology and Experimental Medicine, the 306th Hospital of People’s Liberation Army, Beijing 100101, China |
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Abstract The differentiation of periodontal ligament (PDL) progenitor cells is important for maintaining the homeostasis of PDL tissue and alveolar bone. Vitamin C (VC), a water-soluble nutrient that cannot be biosynthesized by humans, is vital for mesenchymal stem cells differentiation and plays an important role in bone remodeling. Therefore, the objective of this study was to determine the function and mechanism of VC in PDL progenitor cells osteogenic differentiation at the molecular level. We demonstrated that VC could induce the osteogenic differentiation and maturation of PDL progenitor cell without other osteogenic agents. During the process, VC preferentially activated ERK1/2 but did not affect JNK or p38. Co-treatment with ERK inhibitor effectively decreased the Vitamin C-induced expression of Runx2. ERK inhibitor also abrogated Vitamin C-induced the minimized nodules formation. PELP1, a nuclear receptor co-regulator, was up-regulated under VC treatment. PELP1 knockdown inhibited ERK phosphorylation. The overexpression of PELP1 had a positive relationship with Runx2 expression. Taken together, we could make a conclude that VC induces the osteogenic differentiation of PDL progenitor cells via PELP1-ERK axis. Our fi nding implies that VC may have a potential in the regeneration medicine and application to periodontitis treatment.
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| Keywords
periodontal ligament progenitor cells
Vitamin C
PELP1
ERK signaling pathway
osteogenic differentiation
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Corresponding Author(s):
Liang Wei,Email:weixx@sun5.ibp.ac.cn; Niu Zhongying,Email:nzhongying@gmail.com
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Issue Date: 01 August 2013
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