miR-27b inhibits gastric cancer metastasis by targeting NR2F2

doi:10.1007/s13238-016-0340-z

Protein Cell

2017, Vol. 8

Issue (2) : 114-122 https://doi.org/10.1007/s13238-016-0340-z

RESEARCH ARTICLE

miR-27b inhibits gastric cancer metastasis by targeting NR2F2

Qingzhao Feng¹(

),Xionglin Wu⁵,Fuchao Li²,Beibei Ning⁴,Xiaofeng Lu¹,Yin Zhang¹,Ying Pan³,Wenxian Guan¹(

)

¹. Department of General Surgery, The Affiliated Drum Tower Hospital of Medical School of Nanjing University, Nanjing 210008, China
². Department of Gerontology, The Affiliated Drum Tower Hospital of Medical School of Nanjing University, Nanjing 210008, China
³. Department of Orthopaedics, The Affiliated Drum Tower Hospital of Medical School of Nanjing University, Nanjing 210008, China
⁴. The Children’s Hospital of Zhejiang University School of Medicine, Hangzhou 310003, China
⁵. Department of Emergency, The Yiwu Affiliated Hospital of Wenzhou Medical University, Yiwu 322000, China

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Abstract

Increasing attention is focused on the down-regulation of miRNAs in cancer process. Nuclear receptor subfamily 2 (NR2F2, also known as COUP-TFII) is involved in the development of many types of cancers, but its role in gastric cancer remains elusive. In this experiment, oncomine and Kaplan-meier database revealed that NR2F2 was up-regulated in gastric cancer and that the high NR2F2 expression contributed to poor survival. MicroRNA-27b was targeted and down-regulated by NR2F2 in human gastric cancer tissues and cells. The ectopic expression of miR-27b inhibited gastric cancer cell proliferation and tumor growth in vitroand in vivo. Assays suggested that the overexpression of miR-27b could promote MGC-803 cells’ migration and invasion and retard their metastasis to the liver. In addition, down-regulation of miR-27b enhanced GES-1 cells’ proliferation and metastasis in vitro. These findings reveal that miR-27b is a tumor suppressor in gastric cancer and a biomarker for improving patients’ survival.

Keywords miR-27b NR2F2 gastric cancer tumor metastasis

Corresponding Author(s): Qingzhao Feng,Wenxian Guan

Issue Date: 17 March 2017

Cite this article:

Qingzhao Feng,Xionglin Wu,Fuchao Li, et al. miR-27b inhibits gastric cancer metastasis by targeting NR2F2[J]. Protein Cell, 2017, 8(2): 114-122.

URL:

https://academic.hep.com.cn/pac/EN/10.1007/s13238-016-0340-z
https://academic.hep.com.cn/pac/EN/Y2017/V8/I2/114

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