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Protein Cell    2023, Vol. 14 Issue (3) : 180-201    https://doi.org/10.1093/procel/pwac058
RESEARCH ARTICLE
Single-cell transcriptomic atlas of mouse cochlear aging
Guoqiang Sun1,8, Yandong Zheng1,8, Xiaolong Fu3, Weiqi Zhang5,7,8,9,10, Jie Ren5,8,9,10, Shuai Ma2,9,11,12, Shuhui Sun2,9,11, Xiaojuan He4, Qiaoran Wang5,8,10, Zhejun Ji1,9,11, Fang Cheng6,8, Kaowen Yan2,9,11, Ziyi Liu15, Juan Carlos Izpisua Belmonte13, Jing Qu1,8,9,11(), Si Wang4,7,12(), Renjie Chai3,9,14(), Guang-Hui Liu2,4,8,9,11()
1. State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
2. State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
3. State Key Laboratory of Bioelectronics, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, School of Life Sciences and Technology, Southeast University, Nanjing 211189, China
4. Advanced Innovation Center for Human Brain Protection, National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing 100053, China
5. CAS Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China
6. National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
7. Aging Translational Medicine Center, International Center for Aging and Cancer, Beijing Municipal Geriatric Medical Research Center, Xuanwu Hospital, Capital Medical University, Beijing 100053, China
8. University of Chinese Academy of Sciences, Beijing 100049, China
9. Institute for Stem Cell and Regeneration, CAS, Beijing 100101, China
10. China National Center for Bioinformation, Beijing 100101, China
11. Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China
12. The Fifth People's Hospital of Chongqing, Chongqing 400062, China
13. Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA
14. Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China
15. Shandong Provincial Hospital and School of Laboratory Animal Science, Shandong First Medical University, Jinan 250000, China
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Abstract

Progressive functional deterioration in the cochlea is associated with age-related hearing loss (ARHL). However, the cellular and molecular basis underlying cochlear aging remains largely unknown. Here, we established a dynamic single-cell transcriptomic landscape of mouse cochlear aging, in which we characterized aging-associated transcriptomic changes in 27 different cochlear cell types across five different time points. Overall, our analysis pinpoints loss of proteostasis and elevated apoptosis as the hallmark features of cochlear aging, highlights unexpected age-related transcriptional fluctuations in intermediate cells localized in the stria vascularis (SV) and demonstrates that upregulation of endoplasmic reticulum (ER) chaperon protein HSP90AA1 mitigates ER stress-induced damages associated with aging. Our work suggests that targeting unfolded protein response pathways may help alleviate aging-related SV atrophy and hence delay the progression of ARHL.
Keywords single-cell transcriptomic atlas      mouse      cochlea      aging     
Corresponding Author(s): Jing Qu,Si Wang,Renjie Chai,Guang-Hui Liu   
Issue Date: 17 April 2023
 Cite this article:   
Guoqiang Sun,Yandong Zheng,Xiaolong Fu, et al. Single-cell transcriptomic atlas of mouse cochlear aging[J]. Protein Cell, 2023, 14(3): 180-201.
 URL:  
https://academic.hep.com.cn/pac/EN/10.1093/procel/pwac058
https://academic.hep.com.cn/pac/EN/Y2023/V14/I3/180
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