Unraveling fertilization effects on the dynamics of arbuscular mycorrhizal fungal community in the Qinghai-Tibet Alpine Meadow

doi:10.1007/s42832-024-0248-0

2024, Vol. 6

Issue (4) : 240248 https://doi.org/10.1007/s42832-024-0248-0

Unraveling fertilization effects on the dynamics of arbuscular mycorrhizal fungal community in the Qinghai-Tibet Alpine Meadow

Longfei Liu^1,^2,³, Yi Ren², Shuo Sun², Chen Liu², Kairui Ding², Rong Li², Pengfei Zhang^1,⁴(

), Biao Shen², Mohammadhossein Ravanbakhsh⁵, Wu Xiong^2,³(

), Qirong Shen²

¹. College of Ecology, Lanzhou University, Lanzhou 730000, China
². Key Lab of Organic-Based Fertilizers of China, Jiangsu Provincial Key Lab for Solid Organic Waste Utilization, Joint International Research Laboratory of Soil Health, Nanjing Agricultural University, Nanjing 210095, China
³. The Sanya Institute of Nanjing Agricultural University, Sanya 572000, China
⁴. Department of Ecology, Evolution, and Behavior, University of Minnesota, 1475 Gortner Ave, St. Paul, MN 55108, USA
⁵. Ecology and Biodiversity Group, Department of Biology, Institute of Environmental Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

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Abstract

● Community structure and composition of AMF shifted under different fertilization.

● Soil physicochemical properties played important roles in contributing plant diversity and biomass.

● Fertilization affected plant and AMF communities through changing soil abiotic properties.

● Acaulospora and Diversispora were highly linked with plant communities.

Arbuscular mycorrhizal fungi (AMF) represent a crucial component of soil microorganisms, playing pivotal roles in promoting plant growth by enhancing nutrient availability. However, the responses of AMF communities to different fertilization regimes and their correlations with plant communities in the context of anthropogenic disturbances in alpine meadow ecosystems remain largely unexplored. In this study, we investigated the effects of nitrogen, phosphorus, and combined nitrogen-phosphorus fertilization on AMF communities and their interconnections with plant diversity and biomass based on a seven-year long-term experiment conducted on the Qinghai-Tibet Plateau. Our results showed significant shifts in AMF community structure and composition under different fertilization treatments, while the richness of AMF exhibited no remarkable alterations. Notably, soil pH decreased, and electrical conductivity increased with the increasing nitrogen fertilizer application, emerging as pivotal abiotic factors in predicting plant richness and biomass. Fascinatingly, Acaulospora exhibited a positive correlation with plant richness, serving as an important bioindicator of plant richness, while Diversispora emerged as the primary bioindicator of plant biomass. Our findings shed light on potential correlations between AMF community composition and both plant and soil abiotic factors, driven by nitrogen and phosphorus fertilization. We advocate for the critical significance of balanced fertilization in sustaining beneficial plant–soil–AMF interactions in natural ecosystems as well as agricultural soils.

Keywords biodiversity arbuscular mycorrhizal fungi nitrogen and phosphorus fertilizer Qinghai-Tibet Plateau

Corresponding Author(s): Pengfei Zhang,Wu Xiong

Issue Date: 06 May 2024

Cite this article:

Longfei Liu,Yi Ren,Shuo Sun, et al. Unraveling fertilization effects on the dynamics of arbuscular mycorrhizal fungal community in the Qinghai-Tibet Alpine Meadow[J]. Soil Ecology Letters, 2024, 6(4): 240248.

URL:

https://academic.hep.com.cn/sel/EN/10.1007/s42832-024-0248-0
https://academic.hep.com.cn/sel/EN/Y2024/V6/I4/240248

Tab.1 Effects of different fertilizer treatments on soil physicochemical properties.

Fig.1 Effects of different fertilizer treatments on the diversity, community structure and composition of arbuscular mycorrhizal fungi. Richness of AMF community after nitrogen fertilizer (A) and phosphorus fertilizer application (B); Richness of AMF community after phosphorus fertilizer application in high nitrogen treatment (C); PCoA plot of AMF community from different fertilizer treatments (D); Changes in the relative abundance of the AMF genera after nitrogen fertilizer application (E) and phosphorus fertilizer application (F); Changes in the relative abundance of the AMF genera after phosphorus fertilizer application under high nitrogen treatment (G). Different letters indicate a significant difference for the treatments at p<0.05 according to Duncan?s multiple range test. In panels of (E), (F) and (G), only the top three most abundant AMF genera with significant differences for different treatments were shown. CK stands for the unfertilized blank control. N5, N10, N15 represent N addition level of 5, 10, and 15 g N m^?2 year^?1; P2, P4, P8 represent P addition level of 2, 4, and 8 g N m^?2 year^?1; N10P0, N10P2, N10P4, N10P8 represent P addition level of 0, 2, 4 and 8 g P m^?2 year^?1 with 10 g N m^?2 year^?1.

Fig.2 Linkages between arbuscular mycorrhizal fungi, plant communities and soil physicochemical properties. Correlations between plant diversity and biomass, AMF composition (AMF genera) and soil physicochemical properties (A); Relative importance of AMF diversity, AMF genera and soil physicochemical properties in predicting plant richness (B) and biomass (C) by random forest analysis; Linear correlation analysis of the relative abundance of Acaulospora with plant richness (D) and the relative abundance of Diversispora with plant biomass (E) across all the samples. In panels of (A), (B) and (C), * indicates p<0.05, ** indicates p<0.01, *** indicates p<0.001. OM=soil organic matter; TN=soil total nitrogen; TP=soil total phosphorus; AN=soil available nitrogen; AP=soil available nitrogen; EC=soil electrical conductivity.

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[1]	Binghua Han, Xueying Gan, Shunqin Shi, Xueqian Hu, Xianxian Mu, Qiaoling Yu, Shiheng Zhang, Huan Li. Gravesoil fungi are more sensitive than bacteria in response to precipitation[J]. Soil Ecology Letters, 2024, 6(3): 230225-.
[2]	Jiao Ming, Yunge Zhao, Yingying Sun, Zhe Liu. Biocrusts impact soil properties and ecological stoichiometry characteristics in frozen ground regions on the Qinghai-Tibet Plateau[J]. Soil Ecology Letters, 2024, 6(3): 230212-.
[3]	Xiao Li, Duo Zheng, Naili Zhang, Lijia Dong, Aiping Wu, Qiqian Wu, Hua Liu, Mingshui Zhao, Yan Li, Xinping Wang, Yanhong Wang. *Arbuscular mycorrhizal fungi-mediated resistance to salt spray in Cinnamomum camphora* seedlings enhanced by leaf functional traits**[J]. Soil Ecology Letters, 2024, 6(3): 230211-.
[4]	Shuai Du, Xin-Qi Li, Li Bi, Dong Zhu, Hang-Wei Hu, Xiuli Hao, Jiao Feng, Qiaoyun Huang, Yu-Rong Liu. Stochastic community assembly of abundant taxa maintains the relationship of soil biodiversity-multifunctionality under mercury stress[J]. Soil Ecology Letters, 2024, 6(2): 230197-.
[5]	Alexandre Jousset, Seon-Woo Lee. Coming of age for the rhizosphere microbiome transplantation[J]. Soil Ecology Letters, 2023, 5(1): 4-5.
[6]	Anika Lehmann, Eva F. Leifheit, Linshan Feng, Joana Bergmann, Anja Wulf, Matthias C. Rillig. Microplastic fiber and drought effects on plants and soil are only slightly modified by arbuscular mycorrhizal fungi[J]. Soil Ecology Letters, 2022, 4(1): 32-44.
[7]	Mingda Xie, Xinwei Wu, Shucun Sun. Interspecific interactions between burrowing dung beetles and earthworms on yak dung removal and herbage growth in an alpine meadow[J]. Soil Ecology Letters, 2021, 3(2): 94-102.
[8]	Jianing Wang, Xinlan Mei, Zhong Wei, Waseem Raza, Qirong Shen. Effect of bacterial intra-species community interactions on the production and activity of volatile organic compounds[J]. Soil Ecology Letters, 2021, 3(1): 32-41.
[9]	Jing Zhang, Changxin Quan, Lingling Ma, Guowei Chu, Zhanfeng Liu, Xuli Tang. Plant community and soil properties drive arbuscular mycorrhizal fungal diversity: A case study in tropical forests[J]. Soil Ecology Letters, 2021, 3(1): 52-62.
[10]	Jiangnan Li, Peiqin Peng, Jie Zhao. Assessment of soil nematode diversity based on different taxonomic levels and functional groups[J]. Soil Ecology Letters, 2020, 2(1): 33-39.
[11]	Songlin Wu, Xin Zhang, Longbin Huang, Baodong Chen. Arbuscular mycorrhiza and plant chromium tolerance[J]. Soil Ecology Letters, 2019, 1(3-4): 94-104.
[12]	Jiayin Feng, Xinquan Shen, Jian Chen, Jiachun Shi, Jianming Xu, Caixian Tang, Philip C. Brookes, Yan He. Improved rhizoremediation for decabromodiphenyl ether (BDE-209) in E-waste contaminated soils[J]. Soil Ecology Letters, 2019, 1(3-4): 157-173.
[13]	Qing-Lin Chen, Xin-Li An, Bang-Xiao Zheng, Michael Gillings, Josep Peñuelas, Li Cui, Jian-Qiang Su, Yong-Guan Zhu. Loss of soil microbial diversity exacerbates spread of antibiotic resistance[J]. Soil Ecology Letters, 2019, 1(1-2): 3-13.

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