Please wait a minute...
Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2007, Vol. 1 Issue (1) : 67-71     DOI: 10.1007/s11703-007-0012-0
Research article |
Effect of microenvironments and exogenous substance application on 5’-nucleotidase activities in apple peel
Jianguang ZHANG1,Bao DI1,Yingli LI2,Jianqiang ZHANG1(),Jianshe SUN1,Yufang LIU1
1College of Horticulture, Agricultural University of Hebei, Baoding 071001, China
2 College of Sciences, Agricultural University of Hebei, Baoding 071001, China
 Download: PDF(359 KB)   HTML
 Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract

The present experiment was conducted to examine the effect of microenvironments and exogenous substance application on 5’-nucleotidase activity in apple peel tissue. By enclosing apple fruits in bags, treating them with exogenous active oxygen species and regulative agents or placing them under controlled conditions at different fruit temperatures or relative humidity, the 5’-nucleotidase activities were compared with the corresponding controls. The results indicated that, a considerable effect of the microenvironments was found on 5’-nucleotidase activities in fruit peel tissue. The highest enzymatic activity appeared in fruits on the southwest exposure of canopy, regardless of bagged or non-bagged fruits, significantly higher than those from any other exposures. Fruits with bags had a significantly higher 5’-nucleotid-ase activity than the exposed ones. A variation in enzymatic activities was observed in fruits enclosed with different types of bags, which were supposed to alter the microenvironments around them. Within a certain range, gradual or fluctuating rise of fruit temperatures could favor the increase of 5’nucleotidase activities as a result of heat adaptation, whereas the activity would be inhibited if the temperature-rising period was too short or temperature differential was too large. No matter what temperatures fruits were subjected to, high relative humidity was favorable for stimulating the 5’nucleotidase activities, which might partly explain why fruit sunburn would not happen in humid climates. Treatments with four kinds of exogenous active oxygen species could reduce the 5’-nucleotidase activities significantly but spraying with CaCl2 was able to enhance 5’-nucleotidase activities by 55.39%, reaching a 5% significant level.

Keywords microenvironment      exogenous substance      apple      5’-nucleotidase      enzymatic activity     
Issue Date: 22 February 2016
 Cite this article:   
Jianguang ZHANG,Bao DI,Yingli LI, et al. Effect of microenvironments and exogenous substance application on 5’-nucleotidase activities in apple peel[J]. Front. Agric. China, 2007, 1(1): 67-71.
 URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-007-0012-0
http://academic.hep.com.cn/fag/EN/Y2007/V1/I1/67
Fig. 1  Effect of different canopy exposures on 5’-nucleotodase activity in fruit peel

The difference between bagged and control on all exposures reached 1% significant level

Fig. 2  Comparison of fruit surface temperatures between bagged and exposed fruits
Fig. 3  Effect of different types of bags on 5’-nucleotodase activity
Fig. 4  Effect of temperature-rising modes on 5’-nucleotidase activity
Fig. 5  Effect of different humidity on 5’-nucleotidase activity
Fig. 6  Effect of different exogenous active oxygen species on 5’-nucleotidase activity
Fig. 7  Effect of exogenous agents on 5’-nucleotidase activity
[1] Chen S N, Zou X J, Liang B (1996). Cytological translocation of 5’nucleotidase in leaf cells of two rice varieties with different cold-resistance. Acta Batanica Yunnanica, 18(4): 476-478 (in Chinese)
[2] Chen S N, Zou X J, Liang B (1997). Electromicroscope observation on membrane system of leaf cells of some varieties of rice seedlings with different cold-resistance. Plant Physiology Communication, 33(3): 191-194 (in Chinese)
[3] Eastwell K C, Stumpf P K (1982). The presence of 5’-nucleotidase in Swiss chard chloroplasts Beta vulgaris, comparison of nucleotide metabolism in Swiss chard and spinach chloroplasts. Biochemical and Biophysical Research Communications, 108(4): 1690-1694
[4] Li M R, Liu H X, Wang Y R (1996). Effect of low temperature on the activity of 5’-nucleotidase in leaves plasmmatemma of rice seedlings. Plant Physiology Communication, 32(3): 195-197 (in Chinese)
[5] Pan J, Sun L H, Jian L C (1992). Study on biochemistry and cytochemistry of 5’-nucleotidase activity with different cold-resistant rice varieties. Science Bullitin, 37(7): 653-656 (in Chinese)
[6] Pan Z G, Xin P G (1995). Effect of bagging on apple fruit quality and analysis of ecological microenvironments. Northern Horticulture, 101(2): 21-22 (in Chinese)
[7] Simpson J, Rom C R, Patterson M (1998). Causes and possible controls of sunburn on apples. Good Fruit Grower, 39(2): 16-17
[8] Su W A (2000). Plant adaptation to temperature stress. In: Yu S W, Tang Z C, eds. Plant Physiology and Molecular Biochemistry. Beijing: Science Press, 731-732 (in Chinese)
[9] Wang H, Jian L C, Zhang J R (1994). The activity changes of ATPase and 5’-nucleotidase in the cells of rice seedling under low temperature stress. Journal of Electromicroscopy, (3):190-195 (in Chinese)
[10] Yang S A, Endo K (1994). Acclimation temperature affects activities of 5’-nucleotidase and acid phosphatase and lipid and fatty acid composition in carp muscle microsomes. Journal of Food Science, 59(5): 1009-1012
[11] Zhang J G, Liu Y F, Sun J S, Schrader L (2002). Characteristics of fruit surface temperature changes in apple trees under natural conditions. In: Su H R, Li Y N, eds. In: Proceeding of international apple symposium. Tai’an: Journal of Shandong Agricultural University, 88-91
[1] Kejun YUAN, Lixiang Huang, Chengxiang AI, Hairong WEI, Qingzhong LIU. Genomic organization and sequence polymorphism of a farnesyl diphosphate synthase gene in apples (Malus domestica Borkh.)[J]. Front Agric Chin, 2011, 5(2): 209-214.
[2] CAO Shangyin, GUO Junying, CHEN Yuling, XUE Huabai, ZHANG Qiuming, ZHU Zhiyong. Preliminary proteomics analysis of the total proteins of flower bud induction of apple trees[J]. Front. Agric. China, 2008, 2(4): 467-473.
[3] YAO Yuxin, ZHAI Heng, ZHAO Lingling, YI Kai, LIU Zhi, SONG Ye. Analysis of the apple fruit acid/low-acid trait by SSR markers[J]. Front. Agric. China, 2008, 2(4): 463-466.
[4] YAO Yuxin, HAO Yujin, LI Ming, PANG Mingli, LIU Zhi, ZHAI Heng. Gene clone, expression and enzyme activity assay of a cytosolic malate dehydrogenase from apple fruits[J]. Front. Agric. China, 2008, 2(3): 307-313.
[5] YUAN Kejun, LIU Qingzhong, LI Bo, ZHANG Lisi. Genomic structure and sequence polymorphism of E,E-alpha-farnesene synthase gene in apples ( Borkh.)[J]. Front. Agric. China, 2008, 2(2): 190-193.
[6] HAN Mingyu, LI Bingzhi, ZHANG Linsen, BAI Ru, Gao Dengtao. Characteristics of canopy and light transmittance in three types of apple orchards in Weibei areas of Shaanxi Province, China[J]. Front. Agric. China, 2008, 2(1): 93-96.
[7] ZHANG Jianguang, CHEN Shaochun, DI Bao, ZHANG Jianqiang, LIU Yufang, LI Yingli. Effect of high temperature and excessive light on glutathione content in apple peel[J]. Front. Agric. China, 2008, 2(1): 97-102.
[8] ZHENG Weiwei, CHEN Feng, ZHAI Heng, XU Yuehua, ZHANG Jing. Interactive effects of organic fertilizer, CaSO4 and amino acid Ca on Fuji apple in Burozem soil in China[J]. Front. Agric. China, 2007, 1(4): 460-467.
[9] Shuqing LIU,Zhixin YANG,Xiaomin WANG,Xiaogui ZHANG,Rutai GAO,Xia LIU. Effects of Cd and Pb pollution on soil enzymatic activities and soil microbiota[J]. Front. Agric. China, 2007, 1(1): 85-89.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed