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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2007, Vol. 1 Issue (1) : 67-71     DOI: 10.1007/s11703-007-0012-0
Research article |
Effect of microenvironments and exogenous substance application on 5’-nucleotidase activities in apple peel
Jianguang ZHANG1,Bao DI1,Yingli LI2,Jianqiang ZHANG1(),Jianshe SUN1,Yufang LIU1
1College of Horticulture, Agricultural University of Hebei, Baoding 071001, China
2 College of Sciences, Agricultural University of Hebei, Baoding 071001, China
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The present experiment was conducted to examine the effect of microenvironments and exogenous substance application on 5’-nucleotidase activity in apple peel tissue. By enclosing apple fruits in bags, treating them with exogenous active oxygen species and regulative agents or placing them under controlled conditions at different fruit temperatures or relative humidity, the 5’-nucleotidase activities were compared with the corresponding controls. The results indicated that, a considerable effect of the microenvironments was found on 5’-nucleotidase activities in fruit peel tissue. The highest enzymatic activity appeared in fruits on the southwest exposure of canopy, regardless of bagged or non-bagged fruits, significantly higher than those from any other exposures. Fruits with bags had a significantly higher 5’-nucleotid-ase activity than the exposed ones. A variation in enzymatic activities was observed in fruits enclosed with different types of bags, which were supposed to alter the microenvironments around them. Within a certain range, gradual or fluctuating rise of fruit temperatures could favor the increase of 5’nucleotidase activities as a result of heat adaptation, whereas the activity would be inhibited if the temperature-rising period was too short or temperature differential was too large. No matter what temperatures fruits were subjected to, high relative humidity was favorable for stimulating the 5’nucleotidase activities, which might partly explain why fruit sunburn would not happen in humid climates. Treatments with four kinds of exogenous active oxygen species could reduce the 5’-nucleotidase activities significantly but spraying with CaCl2 was able to enhance 5’-nucleotidase activities by 55.39%, reaching a 5% significant level.

Keywords microenvironment      exogenous substance      apple      5’-nucleotidase      enzymatic activity     
Issue Date: 22 February 2016
 Cite this article:   
Jianguang ZHANG,Bao DI,Yingli LI, et al. Effect of microenvironments and exogenous substance application on 5’-nucleotidase activities in apple peel[J]. Front. Agric. China, 2007, 1(1): 67-71.
Fig. 1  Effect of different canopy exposures on 5’-nucleotodase activity in fruit peel

The difference between bagged and control on all exposures reached 1% significant level

Fig. 2  Comparison of fruit surface temperatures between bagged and exposed fruits
Fig. 3  Effect of different types of bags on 5’-nucleotodase activity
Fig. 4  Effect of temperature-rising modes on 5’-nucleotidase activity
Fig. 5  Effect of different humidity on 5’-nucleotidase activity
Fig. 6  Effect of different exogenous active oxygen species on 5’-nucleotidase activity
Fig. 7  Effect of exogenous agents on 5’-nucleotidase activity
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