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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2008, Vol. 2 Issue (1) : 82-92     DOI: 10.1007/s11703-008-0016-4
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Adventitious shoot regeneration from the leaves of some pear varieties ( spp.) grown
ZHANG Yuxing1, DU Guoqiang1, POUDYAL Bharat Kumar2
1.College of Horticulture, Agricultural University of Hebei; 2.Department of Agriculture, Fruit Development Directorate; College of Horticulture, Agricultural University of Hebei
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Abstract The pear (Pyrus spp.) is one of the most important temperate fruit crops. A complete protocol for adventitious shoot regeneration was developed from the leaves of four pear varieties grown in vitro: Abbe Fetel, Yali, Packham’s Triumph and Aikansui, and the Chinese rootstock variety Duli. Shoot explants were collected from the field and cultured in vitro in Murashige and Skoog (MS) media supplemented with 1.0 mg·L-1 6-benzylaminopurine (BA) and 0.1 mg·L-1 indole-3-butyric acid (IBA). After four weeks, leaf explants of all 5 varieties grown in vitro were excised and cultured in MS media supplemented with 0.0 mg·L-1, 0.2 mg·L-1, 0.5 mg·L-1, 1.0 mg·L-1 and 2.0 mg·L-1 naphthaleneacetic acid (NAA) and 5.0 mg·L-1 BA or with 1.0 mg·L-1, 2.0 mg·L-1 and 4.0 mg·L-1 thidiazuron (TDZ). The cultures were maintained in darkness for 21 days for shoot induction in the shoot induction medium (IM), then transferred to the shoot expression medium (EM) in 1.0 mg·L-1 TDZ without any auxins and kept in a growth room at (25 ± 2)°C under a 16/8 h light/dark photoperiod regime for 8 weeks. Finally, the shoots were transferred to the MS shoot elongation medium (SEM) supplemented with 0.2 mg·L-1 BA, 0.1 mg·L-1 IBA and 0.2 mg·L-1 gibberellic acid (GA3). A combination of TDZ and NAA had a significant effect on the number of shoot regenerations in all 5 tested varieties. The maximum mean number of shoots and maximum number of shoots per leaf obtained from Yali variety were 11.8 (P ≤ 0.001) and 22, followed by Aikansui with 6.6 (P ≤ 0.001) and 4.6, and Duli with 8 (P ≤ 0.001) and 12, all arising from the combination of 0.2 mg·L-1 NAA with 1.0 mg·L-1 TDZ. For Packham’s Triumph and Abbe Fetel, the maximum mean number of shoots and maximum number of shoots per leaf were 5.6 (P ≤ 0.001), 4.8 and 8 (P ≤ 0.001), and 11, respectively, from the combination of NAA (1.0 mg·L-1) and TDZ (2.0 mg·L-1). Abbe Fetel was the only variety which produced significantly higher adventitious shoots from the two different combinations of 1.0 mg·L-1 NAA and 5.0 mg·L-1 BA (P ≤ 0.05), and 2.0 mg·L-1 NAA and 5.0 mg·L-1 BA (P ≤ 0.01). Some of the most prominent problems associated with shoot proliferation and regeneration were also observed and discussed in this paper.
Issue Date: 05 March 2008
 Cite this article:   
POUDYAL Bharat Kumar,ZHANG Yuxing,DU Guoqiang. Adventitious shoot regeneration from the leaves of some pear varieties ( spp.) grown [J]. Front. Agric. China, 2008, 2(1): 82-92.
 URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-008-0016-4
http://academic.hep.com.cn/fag/EN/Y2008/V2/I1/82
1 Aliyu O M 2005 Application of tissue culture to Cashew (Anacardium occidentale) breeding: an appraisalAfrican Journal of Biotechnology4(13)14851489
2 Amiri M E 2002 Mass propagation of a unique variety of Pear (Pyrus pyrifolia cv. Sebri) by shoot tip culture in vitro.ActaHort587555561
3 Ana C E Paul M P Charles H M 2006 Adventitious shoot regeneration and rootingof Prunus serotina in vitro cultureHortScience41(1)193201
4 Bell R L 2003 Interactions of genotype and auxin affecting adventitiousregeneration of pearHortScience38(5)750
5 Caboni E lauri P Damiano C D'Angeli S 2000 Somaclonal variation induced by adventitious shoot regeneration inpears and applesActa Hort530195202
6 Cao X 2005 Studies on tissue culture and genetic transformation ofSand Pear. Dissertation for the Master Degree. HangzhouZhejiang University, China610 (in Chinese)
7 Castro M Oyanedel E Cautín R 1995 In vitro shoot proliferation in Avocado (Persea americana) induced by CPPUProceedings of the WorldAvocado Congress III223226
8 Chandra R Padaria J C 1999 Litchi shootbud culture for micropropagationJournalof Applied Hort1(1)3840
9 Gao J Zhang W Ou Y Yang P 2003 Effects on the fast propagation by cultural tissue of pear variety- Xinli No. 7.Journal of Northern Fruits346 (in Chinese)
10 Grazina S Vidmantas S 2004 Plant regenerationfrom leaves of Cydonia oblonga cultivarsActa Universitatis Latviensis, Biology676231133
11 He Q Zhang D Wang R 1995 A preliminary study on preventing brownturning of sucker explants from banana by ascorbic acid pre-treatmentJournal of South China Agricultural University16(3)7982 (in Chinese)
12 Jackson J E 2003 Biology of Apples and PearsCambridgeCambridge UniversityPress22
13 Ju Z 1987 The effects of PPO and its substrates on tissue browningof four pear cultivarsJournal of LaiyangAgricultural College4(2)4247 (in Chinese)
14 Jules J James N M 1996 Pears in fruitbreedingTree and Tropical Fruits1441514
15 Lane W D Iketani H Hayashi T 1998 Shoot regeneration from cultured leavesof Japanese pear (Pyrus pyrifolia)Plant Cell Tiss Org Cult54(1)914
16 Lee C H Kim S B Han D H Kim C S Noh Y M Ban S J Lee D W Lee G P 2004 Shoot organogenesis from leaf explantsin Japanese Pear (Pyrus pyrifolia)Acta Hort653215218
17 Li H X Wang Q C Li C X 1994 Preliminary studies of polyphenoloxidaseactivities and phenol contents in the ear bud and stem terminalsJournal of Sichuan Agricultural University2(2)218222 (in Chinese)
18 Li H X Yan B J 2001 Preliminarystudy on the relationship of oxidases and their isozymes with browningrate of pear explants in tissue cultureJournal of Southwest Agricultural University23(5)432437 (in Chinese)
19 Liu C 2003 The system of in vitro culture and shoot regeneration fromleaves of pear cultures. Dissertation for the Master Degree.Ya'anSichuanAgricultural University5556 (in Chinese)
20 Liu S 2005 Effects of antibiotics on adventitious shoot regenerationfrom in vitro leaves of pear cultivars. Dissertation for the MasterDegree.Ya'anSichuan Agricultural University78 (in Chinese)
21 Marino G Molendini L 2005 In vitro leaf-shootregeneration and somaclone selection for sodium chloride tolerancein quince and pearJournal of HorticulturalScience and Biotechnology80(5)561570
22 Masanori K Yoshiji N 2004 Influencesof carbon sources and their concentrations on shoot proliferationand rooting of ‘Hosui' Japanese pearJournal of Hort Sci39(7)16811683
23 Meghwal P R Sharma H C Singh S K 2000 Effect of surface sterilizing agents onin vitro culture establishment of guava (Psidiumguajava)Journal of Applied Hort2(2)9495
24 Nee C C Tsai C H Anstine D D 2002 Asian pear germplasm - future trends andcurrent research in the industryActa Hort5876169
25 Shaohu T Min S Qigui Z Yun L Daogao L 2005 Establishment of highfrequently regeneration system from leaves of Xueqing' pearActa Hort Sinica32(6)10841087 (in Chinese)
26 Smith J 2000 Micro-propagation of the Gymea lilyKingstonthe Rural Industries Research and Development Corporation
27 Sonia L Dan N Rhonda D C Ping C Stephen H H 2004 Quantitative trait lociassociated with adventitious shoot formation in tissue culture andthe program of shoot development in ArabidopsisGenetics16718831892
28 Sun Q R Davis R E Zhao Y 2005 High frequency regeneration of plantletsfrom leaf explants of commercial pear (Pyruscommunis) cultivars ‘Onward' and ‘Old Home'.The Society for In vitro Biology, Mryland, USA. In Vitro Biology Meeting Abstract49A
29 Sun Q R Liu Q Z Zhao H J Liu P 2004 The study on factors affecting on high frequency shoot regenerationfrom in vitro leaves in pearChinese Agricultural Science Bulletin20(4)99100, 107 (in Chinese)
30 Wang Y L 1996 Chinese PearsBeijingChina Agricultural Scientech Press1
31 Xu L 2001 Studies on setting up the regenerating system of leaf andpetiole of pear. Dissertation for the Master Degree.YanglingNorthwest Sci-Tech Universityof Agriculture and Forestry56 (in Chinese)
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