Please wait a minute...
Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2008, Vol. 2 Issue (2) : 190-193     DOI: 10.1007/s11703-008-0041-3
Genomic structure and sequence polymorphism of E,E-alpha-farnesene synthase gene in apples ( Borkh.)
YUAN Kejun, LIU Qingzhong, LI Bo, ZHANG Lisi
Shandong Institute of Pomology;
 Download: PDF(104 KB)   HTML
 Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract Primer pairs were designed to amplify the genomic DNA sequence of the alpha-farnesene synthase (AFS) gene by PCR. The PCR products were sequenced, spliced and compared to cDNA sequences in the GenBank (accession No. AY182241). The genomic sequence and intron-exon organization of the AFS gene were thus obtained. The AFS genomic sequence has been registered in the GenBank (accession No. DQ901739). It has 6 introns and 7 exons, encoding a protein of 576 amino acids. The sizes of the 6 introns were 108 bp, 113 bp, >1000 bp, 125 bp, 220 bp and 88 bp, and their phases were 0, 1, 2, 2, 0, 0, respectively. The sizes of the deduced amino acids of the 7 exons were 57, 89, 127, 73, 48, 83 and 99, respectively. The AFS protein contained three motifs: the RR(X8)W motif encoded by a sequence in exon 1, and the RxR motif and DDxxD motif encoded by two sequences in exon 4. After comparing the AFS genomic sequence (accession No. DQ901739) to the cDNA sequence (accession No. AY523409) in the GenBank, it was found that there were 6 single-nucleotide polymorphisms between the two sequences, four of which caused mutations at the amino acid level. Interestingly, one amino acid mutation (291R → G) was found in the RxR motif, and further investigation is needed to determine whether the alpha-farnesene synthesis ability and superficial scald susceptibility of apples are influenced by this amino acid mutation and other mutations.
Issue Date: 05 June 2008
 Cite this article:   
YUAN Kejun,LIU Qingzhong,LI Bo, et al. Genomic structure and sequence polymorphism of E,E-alpha-farnesene synthase gene in apples ( Borkh.)[J]. Front. Agric. China, 2008, 2(2): 190-193.
1 Chen J Wang Z Y 2004 Expressionof OsBP-73 gene requires involvement of its intron in riceJournal of Plant Physiology and Molecular Biology30(1)8186 (in Chinese)
2 Chen K S Li F Xu C J Zhang S L Fu C X 2004 An efficient macro-methodof genomic DNA isolation from Actinidia chinensis leavesHereditas (Beijing)26(4)529531 (in Chinese)
3 Davis E M Croteau R 2000 Cyclizationenzymes in the biosynthesis of monoterpenes, sesquiterpenes, and diterpenesTopics in Current Chemistry2095395
4 Hu X S Xiao H Z Wang X X 2002 Contents of α-farnesene and conjugatedtrienes in apples and their relation with storage temperatureActa Horticulturae Sinica31(2)169172 (in Chinese)
5 Ju Z G Curry E A 2000 Lovastatininhibitsα-farnesene synthesis without affecting ethylene productionduring fruit ripening in ‘Golden Supereme' applesJournal of the American Society for HorticulturalScience125(1)105110
6 Kollner T G Schnee C Gershenzon J Degenhardt J 2004 The variability of sesquiterpenes emitted from two Zea mays cultivars is controlled by allelicvariation of two terpene synthase genes encoding stereoselective multipleproduct enzymesThe Plant Cell1611151131.
7 Kuhnlein U Ni L Weigend S Gavora J S Fairfall W Zadworny D 1997 DNA polymorphisms in the chicken growth hormone gene: Responseto selection for disease resistance and association with egg productionAnimal Genetics28116123.
8 Li M Sui N Zhang Y H Meng Q W 2006 Cloning and prokaryotic expression of apple AFS geneActa Horticulturae Sinica33(1)122124 (in Chinese)
9 Pechous S W Watkins C B Whitaker B D 2005 Expression of α-farnesene synthasegene AFS1 in relation to levels of α-farnesene and conjugatedtrienols in peel tissue of scald-susceptible ‘Law Rome' andscald-resistant ‘Idared' apple fruitPostharvest Biology and Technology35125132.
10 Pechous S W Whitaker B D 2004 Cloning andbacterial expression of an (E,E)-α-farnesene synthase cDNA frompeel tissue of apple fruitPlanta2198494.
11 Rupasinghe H P V Paliyath G Murr D P 1998 Biosynthesis of α-farnesene andits relation to superficial scald development in Delicious applesJournal of the American Society for HorticulturalScience123(5)882886
Full text