|
|
Analysis of the apple fruit acid/low-acid trait
by SSR markers |
YAO Yuxin, ZHAI Heng, ZHAO Lingling, YI Kai, LIU Zhi, SONG Ye |
State Key Laboratory of Crop Biology, College of Horticultural Science and Engineering, Shandong Agricultural University; |
|
|
Abstract It is necessary to find out the genetic characteristics of malic acid in the course of apple genomic research and breeding. In this study, the SSR marker linked to the acid/low-acid trait in apple fruit was identified from 140 SSR primer pairs, using 91 F1 population hybrids from the intra-specific cross between apple cultivar ‘Dongguang’ and ‘Fuji’ as the experimental materials. Of 140 SSR primer pairs, only primer SDY085 produced a polymorphic band linked to acid trait, and the linkage distance was 8.89 cM. Also, the titrated acid and malic acid in different developmental stages were determined. The SSR marker analysis, coupled with the change of the total acid and malic acid contents, revealed that the acid/low-acid trait was governed by a major gene and acid trait was completely dominant.
|
Issue Date: 05 December 2008
|
|
1 |
Boubals D, Bourzeix M, Guitraud J (1971). Gora Chirine, an Iranian cv. witha low berry acid levels. Ann Amelior Plantes, 21: 281–285
|
2 |
Cameron J W, Soost R K (1977). Acidityand total soluble solids in Citrus hybrids and advanced crosses involving acidless orange and acidlesspummelo. J Am Soc Hort Sci, 102: 198–201
|
3 |
Fang D Q, Federici C T, Roose M L (1997). Development of molecular markerslinked to a gene controlling fruit acidity in citrus. Genome, 40: 841–849. doi:10.1139/g97-809
|
4 |
Fulton T M, Grandillo S, BeckBunn T, Fridman E, Frampton A, Lopez J, Petiard V, Uhlig J, Zamir D, Tanksley S D (2000). Advanced backcross QTL analysis of a Lycopersicon esculentum × Lycopersicon parviflorum cross. Theor Appl Genet, 100: 1025–1042. doi:10.1007/s001220051384
|
5 |
Li B J, Jing S X, Ding Y Y, Zhang J E (1994). Studies of the inheritance and selection of sweetness and acidityin apples. Acta Genetica Sinica, 21(2): 147–154 (in Chinese)
|
6 |
Liebhard R, Gianfranceschi L, Koller B, Ryder C D, Tarchim R, Van De Weg E, Gessler C (2002). Developmentand characterization of 140 new microsatellites in apple. Molecular Breeding, 10: 217–241. doi:10.1023/A:1020525906332
|
7 |
Liu Z, Yi K, Wang D M, Yang W, Yang F, Zhang J E (2004). Studies on the fruit internal characteristics inheritancetrends of Fuji apple variety crossed progenies. Journal of Fruit Science, 21(2): 95–102 (in Chinese)
|
8 |
Maliepaard C, Alston F H, Arkel G V, Brown L M, Chevreau E, Dunemann F, Evans K M, Gardiner S, Guilford P, Heusden A W V, Janse J, Laurens F, Lynn J R, Manganaris A G, Nijs A P M, Periam N, Rikkerink E, Roche P, Ryder C, Sansavini S, Schmidt H, Tartarini S, Verhaegh J J, Vrielink-van Ginkel M, King G J (1998). Aligning male andfemale linkage maps of apple (Malus pumila Mill.) using multi-allelic markers. Theor Appl Genet, 97: 60–73. doi:10.1007/s001220050867
|
9 |
Monet R (1979). Transmission of the ‘fruit sweetness' character.Incidence on selection for quality. In: INRA, ed. Proceedings of Eucarpiafruit section Symp. Tree Fruit Breed Angers (FRA), 273–276
|
10 |
Nybom T (1959). On the inheritance of acidity in cultivated apples. Heriditas, 45: 332–350
|
11 |
Saliba-Colombani V, Causse M, Langlois D, Philouze J, Buret M (2001). Geneticanalysis of organoleptic quality in fresh market tomato. 1. MappingQTLs for physical and chemical traits. Theor Appl Genet, 102: 259–272. doi:10.1007/s001220051643
|
12 |
Stevens M A (1972). Citrate and malate concentration in tomato fruits: Geneticcontrol and maturational effects. J Am Soc Hortic Sci, 97: 655–658
|
13 |
Sweeney J P, Chapman V J, Hepner P A (1970). Sugar, acid and flavor in fresh fruits. J Am Diet Assoc, 57: 432–435
|
14 |
Visser T, Verhaegh J J (1978). Inheritanceand selection of some fruit characters of apple. Euphytica, 27: 753–760. doi:10.1007/BF00023711
|
15 |
Yoshida M (1970). Genetical studies on the fruit quality of peach varieties.I. Acidity. Bull Hortic Res Stn Jpn Series A, 9: 1–15
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|