Please wait a minute...
Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2008, Vol. 2 Issue (4) : 502-507     DOI: 10.1007/s11703-008-0074-7
|
Sequence of a Cashmere goat type I hair keratin gene and its expression in skin
YIN Jun1, ZHANG Yanjun2, LI Changqing2, LI Jinquan2
1.College of Biotechnology, Inner Mongolia Agricultural University; 2.College of Animal Science and Medical Science, Inner Mongolia Agricultural University;
 Download: PDF(486 KB)   HTML
 Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract The keratin family includes the epithelial (soft) keratins and the hair (hard) keratins, which can be divided into two subfamilies-the acidic type I and the basic or neutral type II. A cDNA is isolated and sequenced from a cDNA library constructed with poly (A) + RNA derived from the goat skin. Because the gene sequence of the known type I hair keratins 8C1 is in accordance with that of the hHa1, this keratin can be identified as the goat hair keratin gHa1 cDNA (GenBank Accession No. AY510110.1). The 413 amino acid sequence derived is compared with the Sheep 8C1 type I mRNA and they exhibit the highest homology (97.8%). In in situ hybridization, it has revealed a strong expression in the cortex of both primary and secondary hair follicles.
Issue Date: 05 December 2008
 Cite this article:   
ZHANG Yanjun,YIN Jun,LI Changqing, et al. Sequence of a Cashmere goat type I hair keratin gene and its expression in skin[J]. Front. Agric. China, 2008, 2(4): 502-507.
 URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-008-0074-7
http://academic.hep.com.cn/fag/EN/Y2008/V2/I4/502
1 Bertolino A P, Checkla D M, Heitner S, Freedberg I M, Yu D W (1990). Differentialexpression of type I hair keratins. J InvestDermatol, 94(3): 297–303.
doi:10.1111/1523-1747.ep12874436
2 Dowling I M, Parry D A D, Sparrow L G (1983). Structural homology between hardα-keratin and the intermediate filament proteins desmin andvimentin. Biosci Rep, 3: 73–78.
doi:10.1007/BF01121573
3 Hanukoglu I, Fuchs E (1982). The cDNAsequence of a human epidermal keratin: divergene of a sequence butconservation of structure among intermediate filament proteins. Cell, 31: 243–252.
doi:10.1016/0092-8674(82)90424-X
4 Heid H W, Moll I, Franke W W (1988). Pattern of expression of trichocyticand epithelial cytokeratins in mammalian tissues. II. Concomitantand mutually exclusive synthesis of trichocytic and epithelial cytokeratinsin diverse human and bovine tissues (hair follicle, nail bed and matrix,lingual papilla and thymic reticulum). Differentiation, 37: 215–230.
doi:10.1111/j.1432-0436.1988.tb00724.x
5 Heid H W, Werner E, Franke W W (1986). The complement of native alpha-keratinpolypeptides of hair-forming cells: a subset of eight polypeptidesthat differ from epithelial cytokeratins. Differentiation, 32: 101–119.
doi:10.1111/j.1432-0436.1986.tb00562.x
6 Kaytes P S, McNab A R, Rea T J, Groppi V, Kawabe T T, Buhl A E, Bertolino A P, Hatzenbuhler N T, Vogeli G (1991). Hair-specific keratins: characterization and expression of a mousetype I keratin gene. J Invest Dermatol, 97(5): 835–42.
doi:10.1111/1523-1747.ep12491511
7 Langbein L, Rogers M A, Winter H, Praetzel S, Beckhaus U, Rackwitz H R (1999). The catalog of human hair keratins. J Biol Chem, 274(28): 19874–19884.
doi:10.1074/jbc.274.28.19874
8 Langbein L, Rogers M A, Winter H, Praetzel S, Schweizer J (2001). The catalogof human hair keratins. II. Expression of the six type II membersin the hair follicle and the combined catalog of human type I andII keratins. J Biol Chem, 276(37): 35123–35132.
doi:10.1074/jbc.M103305200
9 Powell B C, Rogers G E (1986). Hairkeratin composition, structure and biogentsis. In: Bereiter-Hahn J, Matoltsy A G, Riehards K S, eds. Biology of the Integument, Vol2. Berlin: Springer-Verlag, 695–721
10 Steinert P M, Steven A C, Roop D R (1985). The molecular biology of intermediatefilaments. Cell, 42: 411–419.
doi:10.1016/0092-8674(85)90098-4
11 Tohyama C, Nishimura N, Suzuki J S, Karasawa M, Nishimura H (1994). MetallothioneinmRNA in the testis and prostate of the rat detected by digoxygenin-labeledriboprobe, Histochemistry, 101: 341–346.
doi:10.1007/BF00268995
12 Wilson B C, Edwards K J, Sleigh M J, Byrne C R, Ward K A (1988). Complete sequenceof a type I microfibrillar wool keratin gene. Gene, 73: 21–31.
doi:10.1016/0378-1119(88)90309-5
13 Winter H, Siry P, Tobiasch E, Schweizer J (1994). Sequence and expression of murine type I hair keratins mHa2 and mHa3. Exp Cell Res, 212(2): 190–200.
doi:10.1006/excr.1994.1134
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed