Please wait a minute...
Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2008, Vol. 2 Issue (4) : 480-483     DOI: 10.1007/s11703-008-0082-7
|
Suitable condition of enzymatic reactivity for determining the digestibility of feedstuffs phosphorus by dialysis tube method
ZUO Jianjun, DONG Zemin, YAN Xiling, YE Hui, CAO Qingyun, FENG Dingyuan
College of Animal Science, South China Agricultural University
 Download: PDF(72 KB)   HTML
 Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract In order to develop a new method for determining the phosphorus (P) digestibility in vitro in feedstuffs by dialysis tube, a L32(49) orthogonal experiment with eight factors (4 levels for each factor) and a single factor experiment on the enzymatic reactivity were carried out. The sequence of significance of the eight factors on sample-P dialyzability was as follows: trypsin digestion for 6 h, dialyzing solution at 100 mL, pH of pepsin solution at 2.5, pepsin concentration at 2000 UmL-1 pepsin digestion for 100 min, at temperature of 35°C, trypsin concentration at 1625 UmL-1, and pH of trypsin solution at 6.5, respectively. And in vitro dialyzabilities of P in soybean meal, barley, sorghum, peanut meal, and rapeseed meal were (36.91 ± 0.58)%, (27.28 ± 0.94)%, (26.95 ± 0.58)%, (30.51 ± 0.83)%, and (20.82 ± 1.09)%, respectively.
Issue Date: 05 December 2008
 Cite this article:   
DONG Zemin,ZUO Jianjun,YAN Xiling, et al. Suitable condition of enzymatic reactivity for determining the digestibility of feedstuffs phosphorus by dialysis tube method[J]. Front. Agric. China, 2008, 2(4): 480-483.
 URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-008-0082-7
http://academic.hep.com.cn/fag/EN/Y2008/V2/I4/480
1 Fan M Z, Archbold T, Sauer W C, Lackeyram D, Rideout T, Gao Y X, de Lange C F M, Hacker R R (2001). Novelmethodology allows simultaneous measurement of true phosphorus digestibilityand the gastrointestinal endogenous phosphorus outputs in studieswith pigs. J Nutr, 131: 2388–2396
2 Fang R J (2003). True digestibility of phosphorus and predicted modelof truly digestible phosphorus in plant feedstuffs for growing pigs. Dissertation for the Doctoral Degree. Ya'an: SichuanAgricultural University, 60–65 (in Chinese)
3 Gauthier S F, Vachon C, Jones J D, Savoie L (1982). Assessment of protein digestibility by invitro enzymatic hydrolysis with simultaneous dialysis. J Nutr, 112: 1718–1725
4 Huang R L, Li T J, Tan Z L, Xing T X (1999). Study on the suitable condition of enzymatic reactivity for determiningthe disappearance of sample crude protein in vitro by dialysis tube method. Acta Zoonutrimenta Sinica, 11(4): 51–58 (in Chinese)
5 Huo Q G (2002). Animal Nutrition and Source of Phosphorus. Beijing: China Agricultural Science and Technology Press, 114–115 (in Chinese)
6 Ji C, Rong Y, Schenermann S E, Xu W G (1994). Measurement of availability of amino acids in poultry feedstuff bypepsin in vitro. Journal of Agricultural Biotechnology, 2(2): 103–109 (in Chinese)
7 Liu J Z, Ledoux D R, Veum T L (1997). In vitro procedure for predicting the enzymatic dephosphorylation of phytatein corn-soybean meal diets for growing swine. J Agric Food Chem, 45(7): 2612–2617.
doi:10.1021/jf970402z
8 Mauron J, Mottu F, Bujard E, Ejli R H (1955). The availability of lysine, methionine and tryptophan in condensedmilk and milk powder. In vitro digestionstudies. Arch Biochem Biophys, 59: 433–451.
doi:10.1016/0003-9861(55)90510-5
9 Savioe L, Gauthier S F (1986). Dialysiscell for the in vitro measurementof protein digestibility. J Food Sci, 51: 494–498.
doi:10.1111/j.1365-2621.1986.tb11164.x
10 Steinhart H, Kirchgessner H (1973). In vitro verdaungsapparatur zur enzymatischenhydrolyse von proteinen. Arch Tierernahr(Abstract), 23: 449–461
11 Xi P B, Li D F, Zheng C T (2003). Enzymatic conditions of an in vitro method using dialysis tube to studydigestion of protein in rapeseed meal. Journal of China Agricultural University, 8(5): 88–92 (in Chinese)
12 Zhang L Y (2003). Feeding Stuffs-analysis and Determination Technology. Beijing: China Agricultural University Press, 134–136 (in Chinese)
13 Zhang L Y (2003). Feeding Stuffs-analysis and Determination Technology. Beijing: China Agricultural University Press, 136–138 (in Chinese)
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed