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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2009, Vol. 3 Issue (1) : 89-94     DOI: 10.1007/s11703-009-0011-4
Research on Y. schidigera extract feeding on the rumen ecology, protozoal populations and serum chemistries of sheep
Chunlong LIU1,2, Zhongqiu LI3(), Fugang PENG3, Yanming REN4, Zhuolong WANG5
1. Heilongjiang Key Laboratory of Black Soil Ecology,Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China; 2. Animal Nutrition Institute, Northeast Agricultural University, Harbin 150030, China; 3. The Department of Animal Science Research ofHeilongjiang Provincial Agricultural Scientific Academy, Harbin 150086, China; 4. Heilongjiang Institute of Veterinary Drug and Feed Supervisal,Harbin 150009, China; 5. The Station of Animal Quarantine of Mudanjiang City,Mudanjiang 157009, China
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In a completely randomized block design experiment, 16 ruminally cannulated male sheep with body weights of (40 ± 2.1) kg were fed twice daily (8:00 and 16:00) with concentrate and forage (50:50 on dry matter (DM) basis). Dietary treatments were supplemented with intraruminal doses of powdered Yucca schidigera extract (YSE) at the levels of 0 (control), 100, 200 and 300 mg?kg-1. On days of 15, 16 and 17 after feeding, ruminal content was sampled at 0, 2, 4, 6 and 8 h after dosing (8:00), and blood samples were collected at the end of experiment (the days 18 and 19 after feeding). Results showed that the treatment groups’ acidity was not affected (P = 0.13) by YSE. Comparing to the control, the ruminal propionate concentration was increased by YSE addition in a dose-dependent manner by up to 29.8% (P< 0.05), and the acetic concentration was decreased by up to 17.5% (P< 0.05). The ruminal ammonia concentration 2 hours after feeding was higher (P< 0.05) in sheep fed without YSE (increased by 17.57 mg?100 mL-1) than those fed with YSE at 200 mg?kg-1 (6.77 mg?100 mL-1increase in NH3) and at 300 mg?kg-1 (6.50 mg?100 mL-1 increase in NH3). Protozoal populations in the rumen were lower (P< 0.05) with the YSE feeding dose at 300 mg?kg-1 than the control. The serum chemistries were not different among treatments (P > 0.05) and were within the normal physiological ranges for sheep 19 days after feeding. The study indicated that 200 mg?kg-1 and 300 mg?kg-1 YSE groups had particular suppressing effects on ruminal ammonia concentration, ammonia-N concentrations and protozoal populations. The effect of YSE on ruminal fermentation could be attributed to the selective inhibitory effect on rumen microbial species. High level (300 mg?kg-1) YSE as feed additives resulted no negative impact on sheep in our tests.

Keywords Yucca schidigera extract      rumen ecology      protozoal populations      serum chemistries      sheep     
Corresponding Authors: LI Zhongqiu,   
Issue Date: 05 March 2009
URL:     OR
itemLeymus chinensisconcentrateamixed dietb
dry matter (DM)883860871.5
organic matter (OM)844936890
crude protein (CP)80167.4123.7
soluble CP4812687
Tab.1  Chemical composition of , concentrate and mixed diet (g?kg DM)
itemYucca schidigera extract/mg?kg-1S.E.M.effecta (P-value)
0100200300DTD × T
ammonia N/mg?100mL-118.66a18.56a16.01b16.14b1.4520.030.170.53
propionic acid17.02b18.16b19.13b22.09a0.1730.020.520.61
butyric acid6.
total VFA67.4466.9064.3165.341.2700.670.030.12
Tab.2  Effect of YSE on rumen fermentation and protozoa populations
Fig.1  Effects of YSE dietary treatments on pH variation at different time
Fig.2  Effects of YSE dietary treatment on NH-N variation at differents times
0100200300S.E.M.P-valuenormal physiologicalranges for sheep
Tab.3  Effects of different levels of YSE on serum traits of sheep
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