Construction of a genetic linkage map and QTL analysis for some leaf traits in pear (<italic>Pyrus </italic>L.)

doi:10.1007/s11703-009-0013-2

Front Agric Chin

2009, Vol. 3

Issue (1) : 67-74 https://doi.org/10.1007/s11703-009-0013-2

RESEARCH ARTICLE

Construction of a genetic linkage map and QTL analysis for some leaf traits in pear (Pyrus L.)

Wenying SUN¹, Yuxing ZHANG¹(

), Wenying SUN², Wenquan LE³, Hai’e ZHANG³

1. Key Laboratory of Fruit Setting Physiology and Molecular Biology, Agricultural University of Hebei, Baoding 071001, China,; 2. Plant Science Department, Henan Vocational College of Agriculture, Zhengzhou 451450, China; 3. Hebei Changli Institute of Pomology, Changli 066600, China

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Abstract

The major incompatibility barriers to specific inbred lines and the long generation duration in Pyrus L. may hinder the Pyrus breeding process. A genetic linkage map provides the foundation for quantitative trait loci (QTL) mapping and molecular marker-assisted breeding. In this study, we constructed a genetic map with 145 F₁ populations from a cross of two cultivars, Yali and Jingbaili, using AFLP and SSR markers. The map consisted of 18 linkage groups which included 402 genetic markers and covered 1395.9 cM, with an average genetic distance of 3.8 cM. The interval mapping was used to identify quantitative trait loci associated with four leaf agronomic traits in the F₁ population. The results indicated that four QTLs were associated with leaf length, two QTLs with leaf width, two with leaf length/leaf width, and three with petiole length. The eleven QTLs were associated with 9.9%-48.5% of the phenotypic variation in different traits. It is considered that the map covers almost the whole genome, and molecular markers will be greatly helpful to the related breeding.

Keywords Pyrus L molecular linkage map QTL analysis leaf traits

Corresponding Author(s): ZHANG Yuxing,Email:jonsonzhyx@yahoo.com.cn

Issue Date: 05 March 2009

Cite this article:

Wenying SUN,Yuxing ZHANG,Wenying SUN, et al. Construction of a genetic linkage map and QTL analysis for some leaf traits in pear (Pyrus L.)[J]. Front Agric Chin, 2009, 3(1): 67-74.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-009-0013-2
https://academic.hep.com.cn/fag/EN/Y2009/V3/I1/67

Fig.1

Fig.2

Fig.3 Molecular genetic maps in pear based on AFLP and SSR markers

Fig.4 The frequency distribution of four leaf traits in pear mapping population

Tab.1 The QTLs distribution of 4 leaf traits in pear genetic linkage map

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