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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2009, Vol. 3 Issue (2) : 152-158     DOI: 10.1007/s11703-009-0024-z
RESEARCH ARTICLE |
Symptoms and histopathological study of Anoplophora glabripennis larvae infected with Metarhizium (Metsch.) Sorokin MS01
Baohui WANG, Jianwei ZHENG, Dazhuang HUANG(), Da WANG, Xiaoyong HAN, Xiaohong WANG
College of Forestry, Agricultural University of Hebei, Baoding 071000, China
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Abstract  

Some changes in color and morphology of Anoplophora glabripennis larvae were observed when infected with Metarhizium (Metsch.) Sorokin MS01. Paraffin sections show the spores of MS01 attached to the cuticle of A. glabripennis larvae at 24 h post inoculation (hpi). At 36 hpi, the conidium germinated and penetrated through the cuticle. After 48 h of inoculation, the endocuticle was disintegrated and the germ tubes passed through the epithelial layers into the hemocoel and multiplied, and then invaded the tissues near the penetration point, such as the fat body, muscle, tracheal tissue, malpighian tubule, alimentary canal, etc. The infected larvae were dead and most tissues and organs were infected and disintegrated with the multiplication of the mycelium for 3 days. The hyphae emerged through the cuticle and formed a hyphal layer on the surface of the dead insect and the conidium emerged five days after inoculation.

Keywords Anoplophora glabripennis      larvae      Metarhizium (Metsch.) Sorokin      symptom      histopathology     
Corresponding Authors: HUANG Dazhuang,Email:huangdazhuang@126.com   
Issue Date: 05 June 2009
URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-009-0024-z     OR     http://academic.hep.com.cn/fag/EN/Y2009/V3/I2/152
Fig.1  Symptom of larvae infected with strain MS01
Note: (a), (b) and (c) represent the soaked spots on the cuticle of infected larvae, the white mycelium on the cuticle of infected larvae, and the green conidia on the cuticle of infected larvae, respectively.
Fig.2  The histopathological changes of integument of larvae infected with strain MS01(×160)
Note: (a) indicates healthy cuticle; (b) indicates cuticle of infected larvae separated from epidermis at 48 hpi; (c) indicates bristle of infected larvae fell at 72 hpi.
Fig.3  The histopathological changes of trachea of larvae infected with strain MS01 (×160)
Note: (a) indicates healthy trachea; (b) indicates that the taenidium of infected larvae was damaged and the cavity emerged at 48 hpi; (c) indicates the taenidium of infected larvae slowly disappeared at 96 hpi.
Fig.4  The histopathologic changes of fat body of larvae infected with strain MS01 (×160)
Note: (a), (b) and (c) represent healthy fat body, incompact fat body of infected larvae at 48 hpi, and fat body of infected larvae covered up with hyphae at 120 hpi, respectively.
Fig.5  Healthy muscle tissue ((a), (c)) and a crack in the loose muscle tissue of infected larvae at 72 hpi ((b), (d)) (×160)
Fig.6  Histopathologic changes of malpighian tubes of larvae infected with strain MS01 (×800)
Note: (a) stands for normal malpighian tubes showing a small diameter; (b) stands for a malpighian tube of infected larvae showing prominent extermal perimeter at 72 hpi; (c) stands for malpighian tubes of infected larvae showing large diameter and covered up with hyphae at 96 hpi.
Fig.7  Histopathological changes of the alimentary canal of larvae infected with strain MS01
Note: (a), (b), (c), (d), (e) and (f) represent healthy alimentary canal (×160), peritrophic membrane of infected larvae disappeared at 72 hpi (×160), the disorganizing alimentary canal at 120 hpi (×160), healthy mid-gut wall cells and peritrophic membrane (×640) columned cells vacuoled in the alimentary canal at 96 hpi (×640) and remnant alimentary canal at 120 hpi (×640), respectively.
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