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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2009, Vol. 3 Issue (2) : 164-170     DOI: 10.1007/s11703-009-0026-x
RESEARCH ARTICLE |
Mycorrhizal and dark septate endophytic fungi under the canopies of desert plants in Mu Us Sandy Land of China
Yanqing WU1, Xueli HE1(), Tiantian LIU2
1. College of Life Sciences, Hebei University, Baoding 071002, China; 2. College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China
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Abstract  

Biodiversity of arbuscular mycorrhizal colonization and spore density was investigated in 20 desert plants (dominant or common species) collected from different locations of Mu Us Sandy Land of China. We observed three mycorrhizal types including Arum-type,Paris-type, and an intermediate type among the plants. Another type of potentially beneficial fungi associated with roots of all species was also observed, namely, dark septate endophytic fungi (DSEF). Of the 20 sample plants examined, all species were coinfected by the two target fungi (Arbuscular mycorrhizal fungi (AMF) colonization of Salix psammophila and DSEF colonization of Periploca sepium were as low as 4%). Based on this investigation, we speculated that the DSEF are ubiquitous in desert ecosystems and can co-occur with AMF in desert plants, functioning much like mycorrhizal fungi. Further studies will be required to elucidate interactional mechanisms with AMF and the mechanisms operating in desert ecosystem.

Keywords Arbuscular mycorrhizal fungi      mycorrhiza type      root endophytic fungi      desert plants     
Corresponding Authors: HE Xueli,Email:xuelh1256@yahoo.com.cn   
Issue Date: 05 June 2009
URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-009-0026-x     OR     http://academic.hep.com.cn/fag/EN/Y2009/V3/I2/164
plant samplesite description
familyspecieslife formroot typenaturelocationlong. (E)lat. (N)altitude/m
SalicaceaeS. psammophilashrubtap rootnoYC107°10′054″37°54′333″1460
TamaricaceaeT. chinensisshrubtap rootnoYC107°10′054″37°54′333″1460
LeguminosaeC. intermediashrubtap rootnoYC107°10′054″37°54′333″1460
C. microphyllashrubtap rootnoYC109°57′733″40°13′175″1206
C. korshinskiishrubtap rootnoYC105°4′293″37°31′418″1255
A. mongolicusshrubtap rootyesALS104°58′099″37°54′333″1460
O. aciphyllashrubtap rootyesSPT104°48′406″37°26′799″1592
H. scopariumshrubtap rootyesSPT104°56′075″37°26′372″1360
H. fruticosumperennial herbtap rootnoYJZ110°11′226″39°29′401″1280
A. adsurgensperennial herbtap rootyesYC107°10′054″37°54′333″1460
G. uralensisperennial herbtap rootyesYC107°10′054″37°54′333″1460
ZygophyllaceaeS. xanthoxylumshrubtap rootnoYC107°10′054″37°54′333″1460
AsclepiadaceaeP. sepiumshrubfibrous rootyesALS104°58′099″37°43′573″1357
CompositaeA. ordosicasemi-shrubtap rootyesYC107°10′054″37°54′333″1460
A. sphaerocephalasemi-shrubtap rootyesRSCF109°42′541″38°20′072″1100
A. scopariaperennial herbtap rootyesRSCF109°42′541″38°20′072″1100
GramineaeS. capillataperennial herbfibrous rootyesSPT107°3′674″37°59′129″1415
P. villosperennial herbfibrous rootyesSPT104°56′075″37°26′372″1360
P. austiralisperennial herbfibrous rootyesSPT105°03′853″37°31′883″1242
LiliaceaeA . mongolicumbiennial herbfibrous rootyesSPT104°48′406″37°26′799″1592
Tab.1  Description of site and plant sampling
Fig.1  Mycorrhizal types
Note: (a), (b) and (c) represent Paris-type of , intermediate type of , and coexistence of AMF and DSEF in respectively. H, HC, V, HH and MS represent hyphae of AMF, hyphal coils of AMF, vesicles of AMF, hyaline hypha of DSEF and microsclerotium, respectively.
plant speciesmycorrhiza typeshyphae∕%vesicle∕%arbuscule∕%total AM & DSE/%DSE∕%MS∕%total AM∕%spore density/(spore number·g-1 dry soil)
SalicaceaeS. psammophilaI440410012434.75
TamaricaceaeT. chinensisA68280242486833.00
LeguminosaeC. intermediaI87971067535010063.25
C. microphyllaI956725247131005.36
C. korshinskii.I91602544022918.94
A. mongolicumP8800241868816.50
O. aciphyllaP589205858429233.45
H. scopariumI856507575308511.20
H. fruticosumA8083093977977.50
A. adsurgensP80400102008026.50
G. uralensisP1020027100302010.85
ZygophyllaceaeS. xanthoxylumA606704033206756.00
AsclepiadaceaeP. sepiumI962008441008.20
CompositaeA. ordosicaP563244048126474.60
A. sphaerocephalaP83330333308312.40
A. scopariaA30400102505039.25
GramineaeS. capillataP8590070701010083.60
P. villosaP23430439720462.80
P. australisA28280283328332.450
LiliaceaeA. mongolicumI06706773276716.05
Tab.2  Status of different structures and spore density of AMF and DSEF tissues in the arid plots
Fig.2  Colonization by AMF and DSEF in tissues
Note: (a) represents superficial hyphae of AMF (AMH) and DSE (DH) on lateral roots of . fruticosum. (b) represents hyhae of AMF (AMH) through the cell wall in the roots of . (c) represents superficial hyphae of DSE on roots of . (d) represents vesicles of AMF in the roots of . (e) represents vesicles of AMF in the roots of . (f) represents arbuscule (Ar) of AMF in the roots of . (g) represents vesicles of AMF in the roots of . (h) represents superficial hyphae of DSE and microsclerotia (MS) on the roots of ((a), bar=100 μm ; (b), (c), (d), (e), (f), (g) and (h), bar = 25 μm).
itemvesiclearbusculartotal AMspore densityDSEmicrosclerotium
hyphae0.2930.2570.869**0.076-0.491*-0.192
vesicle0.3600.578**0.3070.2330.491*
arbuscule0.2700.418-0.0350.488*
total AM0.103-0.376-0.025
spore density-0.0540.068
DSE0.363
Tab.3  Correlations of different AM structures and DSE tissues
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