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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2009, Vol. 3 Issue (3) : 279-283     DOI: 10.1007/s11703-009-0060-8
RESEARCH ARTICLE |
Somatic embryogenesis and plant regeneration in glandless upland cotton (Gossypium hirsutum L.)
Jingyan SUN1, Weiming LI1(), Hanshuang ZHANG1, Junli ZHAO1, Xiaolin YIN1, Li’an WANG2
1. Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China; 2. College of Life Science, Hebei Normal University, Shijiazhuang 050016, China
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Abstract  

Glandless upland cotton has an important economic value. Embryogenic calli and regenerated plants were obtained from the hypocotyl explants of glandless upland cotton seedlings, cultivar Jisheng1. The results indicated that somatic embryogenesis was significantly influenced by the types of auxin and cytokinin. 2, 4-D was advantageous to induce cotton callus, but embryogenic callus could not be obtained on the 2, 4-D medium. Embryogenic calli were also not obtained on the MSB sold medium with the combination of IBA and BA. However, embryogenic calli were induced when the hypocotyl explants were cultured on the IBA and KT medium. More than 31% of the hypocotyl segments produced embryogenic calli when the MSB medium was supplemented with 1.0 mg·L-1 IBA and 0.5 mg·L-1 KT. Embryogenic calli with somatic embryos could be observed within three months. Somatic embryo germination and maturation occurred on the hormone-free MSB medium with 1.0 g·L-1 Gln and 0.5 g·L-1 Asn. A number of regenerated plants could be obtained in six months. In the present study, a simple and efficient system was established to induce a number of embryogenic calli and regenerate plantlets from hypocotyl explants.

Keywords cotton (Gossypium hirsutum L.)      glandless      somatic embryogenesis      plant regeneration     
Corresponding Authors: LI Weiming,Email:li_wyman@yahoo.com   
Issue Date: 05 September 2009
URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-009-0060-8     OR     http://academic.hep.com.cn/fag/EN/Y2009/V3/I3/279
treatmentNHSPCIRC/%weight of callus/gNHSPECIREC/%color and texture of callus
1120a100a53.8a0b0bwhite, soft unfriable
2120a100a53.5a0b0bwhite, soft unfriable
3100b83.3b28.2b0b0bdark, hard compact
467c55.8c13.5c38a31.7ayellowish, friable
5120a100a53.7a0b0bwhite, hard unfriable
Tab.1  Effects of hormone combinations and measures on the induction of embryogenic callus
Fig.1  Somatic embryogenesis of the glandless upland cotton
Note: (a)–(f) represent callus induced on the medium with the combination of 2,4-D, IAA and KT, callus induced on the medium with the combination of IBA and BA, callus induced on the medium with the combination of IBA and KT, embryogenic callus, the germination of somatic embryos and regenerated plants of Jisheng1, respectively.
Fig.2  Effect of the concentrations of the IBA and KT on the induction rate of callus and embryogenic callus
treatmentGln/(g·L-1)Asn/(g·L-1)embryo conversion/%
medium 1006.3d
medium 20.50.511.8c
medium 30.51.022.6b
medium 41.00.538.5a
medium 51.01.037.9a
Tab.2  The effects of the concentrations of glutamine and asparagine on the germination of embryo
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[1] LI Zhikun, WANG Xingfen, ZHANG Yan, ZHANG Guiyin, WU Liqiang, CHI Jina, Zhiying MA. Assessment of genetic diversity in glandless cotton germplasm resources by using agronomic traits and molecular markers[J]. Front. Agric. China, 2008, 2(3): 245-252.
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