|
|
|
The effects of phlorizin stress on the protective
enzyme and metabolic regulation substances in the root of M. micromalus |
| Xiaojing CUI,Yanan WANG,Wenchao ZHEN, |
| College of Plant Protection,
Agricultural University of Hebei, Research Center for Biocontrol Techniques
Against Pests on Crops of Hebei Province, Baoding 071001, China; |
|
|
|
|
Abstract The effects of phlorizin stress on the protective enzyme (PE) and metabolic regulation substances (MRS) in the Malus micromalus root were studied by tissue culture method. Under four concentration gradients of phlorizin stress, the activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), the content of malondialdehyde (MDA), proline (Pro) and soluble proteins were tested. The results showed that phlorizin stress could lead to different changing tendencies for different detection indicators. The changes of SOD activity, POD activity and Pro content appeared more evident. The difference between the treatments under phlorizin stress and the control without phlorizin stress was not significant in the initial period. With the extension of processing time the difference became more and more evident and the protective enzyme activities and metabolic regulation substances were influenced greatly under different concentrations of phlorizin stress. When the concentration of phlorizin got to 1g·L−1, the effect was the most obvious. The activities of protective enzymes were significantly improved, with the MDA production reduced, and Pro and soluble protein (SP) accumulation accelerated.
|
|
Issue Date: 05 September 2010
|
|
|
Bai R, Zhao X, Ma F W, Li C Y (2009). Identification and bioassay of allelopathic substancesfrom the root exudates of Malus prunifolia. Allelopathy Journal, 23(2): 477–484
|
|
Evans H C (1997). Parthenium hysterophorus: a reviewof its weed status and the possibilities for biological control. Biocontrol News and Information, 18(3): 89–98
|
|
Liang Y, Chen Q, Liu Q, Zhang W, Ding R (2003). Exogenoussilicon (Si) increases antioxidant enzyme activity and reduces lipidperoxidation in roots of salt-stressed barley (L.). J Plant Physiol, 160(10): 1157–1164
doi: 10.1078/0176-1617-01065
|
|
Liu J P, Zheng C M (2002). Application of in vitro selection and somaclonal variation in improvementof disease resistance. Hereditas, 24(5): 617–630 (in Chinese)
|
|
Lu R J, Wang Y F, Sun Y F (2006). Selection of broccoli variants tolerant to higher temperature using haploid stem apexes in vitro. Acta Agriculturae Nucleatae Sinica, 20(5): 388–391
|
|
Lu W G, Zhang C L,Yuan F, Peng Y(2000). Preliminary study on relieving the obstacles of continuouscropping cucumber in greenhouse by inoculating. Acta Agriculturae Boreali-Sinica, S1: 153–156 (in Chinese)
|
|
Marci R, Vianello A, Pennazio S (1986). Salicylate-collapsed membrane potential in pea stem Mitoehdria. Physilogia plantarum, 67:136–140.
|
|
Sanderson M A, Skinner R H, Barker D J (2004). Plant species diversity and management of temperate forage and grazing landecosystems. Crop Science Society of America, 44(4): 1132–1144
|
|
Santos W D, Ferrarese M L, Finger A, Teixeira A C N, Ferrarese-Filho O (2004). Lignification and related enzymes in Glycine max root growth-inhibition by ferulic acid. J Chem Ecol, 30(6): 1203–1212
doi: 10.1023/B:JOEC.0000030272.83794.f0
|
|
Schieber A, Keller P, Carle R (2001). Determination of phenolic acids and flavonoids of apple and pear by high-performanceliquid chromatography. J Chromatogr A, 910(2): 265–273
doi: 10.1016/S0021-9673(00)01217-6
|
|
Shu H R (1993). Physiologist of fruit trees in cultivation. Beijing, China Agriculture Press (in Chinese)
|
|
Wang Y F, Huang J H, Lu R J (2002). In vitro selection of rape variants resistant to oxalic acid using haploid stem apexes. Acta Agriculturae Nucleatae Sinica, 16(6):355–359 (in Chinese)
|
|
Wu F Z, Zhao F Y, Ma F M (2001). Phenolic acid substances and Allelopathy mechanisms. Journal of Northeast Agricultural University, 32: 313–319 (in Chinese)
|
|
Wu F Z, Huang C H, Zhao F Y (2002). Effects of phenolic acids on growth and activities of membrance protective enzymes ofcucumber seedlings. Scientia AgriculturaSinica, 35: 821–825 (in Chinese)
|
|
Xu J H, Li R Q, Wang J B (1995). Some changes of enzyme activities from susceptible and resistant cucumber cultivars afterinoculation with cucumber wilt fusarium. Acta Phytopathologica Sinica, 25(3): 239–241(in Chinese)
|
|
Yang X H, Wang S H, Wu X Y (1994). Apple replant disease and the identification of pathogenic nematode species. Acta Phytopathologica Sinica, 24(2): 165–168 (in Chinese)
|
|
Yu J Q, Ye S F, Zhang M F, Hu W H (2003). Effects of root exudates and aqueous root extracts ofcucumber (Cucumis sativus) and allelochemicalson photosynthesis and antioxidant enzymes in cucumber. Biochemical Systematics and Ecology, 31(2): 129–139
doi: 10.1016/S0305-1978(02)00150-3
|
|
Zhang L (2002). Studies on screening techniques forsalt tolerance evaluation of induced mutants of apple stock in vitro. Dissertation for the Master Degree. Baoding: Agricultural University of Hebei (in Chinese)
|
|
Zhang J H, Shu H R, Wang L Q (2005). Allelopathic effect of phenolics and its role on apple replant disease mechanism. Dissertationfor the Doctoral Degree. Taian: Shandong Agricultural University, 64–65
|
|
Zhang J H, Mao Z Q, Wang L Q, Shu H R (2007). Effect of phloridzin on physiological characteristicsof malus hupehensis Rehd seedlings. Scientia Agricultura Sinica, 40(3): 492–498 (in Chinese)
|
|
Zhang X L, Pan Z G, Zhou X F (2007). Autotoxicity and continuous cropping obstacles: a review. Chinese Journal of Soil Science, 38(4): 782–783 (in Chinese)
|
|
Zhang X Z (1992). Research Methods of Crop Physiology. Bejing: China Agricultural Press, 139–145 (in Chinese)
|
|
Zou Q (1995). The Plant Physiology BiochemistryExperiment Instructs. Beijing: China Agricultural Press, 94–99(in Chinese)
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
