Please wait a minute...
Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2011, Vol. 5 Issue (3) : 253-261     DOI: 10.1007/s11703-011-1069-3
Effects of chromosome substitution on the utilization efficiency of nitrogen, phosphorus, and potassium in wheat
Chengjin GUO1, Jincai LI2, Wensuo CHANG1, Lijun ZHANG1, Xirong CUI1, Shuwen LI3, Kai XIAO1()
1. College of Agronomy, Agricultural University of Hebei, Baoding 071001, China; 2. Administration Office of Science and Technology, Agricultural University of Hebei, Baoding 071001, China; 3. College of Resource and Environment, Agricultural University of Hebei, Baoding 071001, China
Download: PDF(306 KB)   HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks

A complete set of chromosome substitution lines with genetic background of Chinese Spring (CS) were used to determine the effects of each chromosome on utilization efficiencies of nitrogen, phosphorus, and potassium in wheat (Triticum aestivum L.). In each line, only one pair of chromosomes in CS genome was substituted by the corresponding one of donor Synthetic 6x. Under normal growth conditions supplied with enough inorganic nutrients, the dry mass per plant and the utilization efficiencies of nitrogen (N), phosphorus (P), and potassium (K) in plants varied largely among CS, Synthetic 6x, and the chromosome substitution lines (1A–7A, 1B–7B, and 1D–7D). Of these, 1A substituted by the chromosome 1A of Synthetic 6x (other lines are the same as 1A hereafter) had the highest plant dry mass and the accumulative amount of N and K, and 1B behaved to have the highest plant accumulative P amount. 1D and 4D had the lowest accumulative P amount and plant dry mass, respectively. 4B showed the lowest plant accumulative N and K. Thus, chromosome 1A of Synthetic 6x contains major genes endowing plant capacities of higher dry mass, accumulative N and K, whereas chromosome 1B of Synthetic 6x carries major genes improving plant accumulative P capacities. The lines, together with CS and the donor, could be classified into three groups including high-efficiency, mid-efficiency, and low-efficiency based on plant dry mass. Regression analysis suggested that there are significantly positive correlations between plant dry mass and the accumulated amount of N, P, and K. Further, there are positively significant correlations among the plant accumulative N amount and some plant traits and physiological parameters, as well as positively significant correlations between the accumulative amount of P and K and the photosynthetic rate (Pn).

Keywords wheat (Triticum aestivum L.)      chromosome substitution line      nitrogen efficiency      phosphorus efficiency      potassium efficiency      plant growth trait      photosynthetic parameter     
Corresponding Authors: XIAO Kai,   
Issue Date: 05 September 2011
URL:     OR
Fig.1  The dry mass and accumulated amount of N, P, and K in plants of CSLs, CS, and Synthetic 6x. Chinese Spring (CS) and Synthetic 6x are used as the recipient and donor of chromosomes, respectively. 1A to 7A, 1B to 7B, and 1D to 7D are chromosome substitution lines (CSLs) with just only a single pair of chromosome in CS substituted by the corresponding one of Synthetic 6x. Fig. 1A is the plant dry mass of CS, Synthetic 6x, and CSLs. The data shown are average±standard errors (). Fig. 1B is the plant accumulative N of CSLs, CS, and Synthetic 6x. Fig. 1C is the plant accumulative P of CSLs, CS, and Synthetic 6x. Fig. 1D is the plant accumulative K of CSLs, CS, and Synthetic 6x.
Fig.2  Regression analysis of plant accumulative amount of N, P, and K based on plant dry mass and nutrient utilization traits derived from CSLs, CS, and Synthetic 6x. A, D, and G show the results of regression analyses between plant accumulative N amount and plant dry mass (A), N content (D), and N efficiency (G), respectively. B, E, and H show the results of regression analyses between plant accumulative P amount and plant dry mass (B), P content (E), and P efficiency (H), respectively. C, F, and I show the results of regression analyses between plant accumulative K amount and plant dry mass (C), K content (F), and K efficiency (I), respectively. * and ** indicate that the regression coefficient reaches significant levels of 5% and 1%, respectively.
LinesDry mass (mg per plant)N content (%)P content (%)K content (%)Accumulative N (mg per plant)Accumulative P (mg per plant)Accumulative K (mg per plant)N efficiency (mg per mg N)P efficiency (mg per mg P)K efficiency (mg per mg K)
Synthetic 6x68b6.47a1.91a0.69b4.40b1.29a0.46b15.45b52.71c147.83ab
A-D Ave68.67b5.51b0.91bc0.69b3.78c0.63b0.47b18.22a113.34b146.02ab
A-D SE8.210.300.170.040.520.150.071.0119.849.68
A-DCV (%)11.965.3818.876.0013.7724.6414.965.5717.506.63
A Ave69.14b5.65b0.97b0.71b3.90c0.67b0.49b17.75a104.20b140.92b
A SE8.380.
A CV (%)12.113.767.195.0413.6614.8815.223.677.154.78
B Ave68.71b5.42b0.98b0.69b3.72c0.68b0.48b18.52a107.05b144.79ab
B SE7.890.340.250.040.430.210.071.1920.928.12
B CV (%)11.486.3125.055.1811.6131.6914.566.4019.545.61
D Ave68.14b5.45b0.79c0.66b3.73c0.54c0.45b18.39a128.76a152.35a
D SE9.580.310.100.040.640.100.071.1019.9411.04
D CV (%)14.065.6812.036.2617.1618.2815.975.9715.497.25
Tab.1  Plant day masses and nutrient utilization traits of the CSLs, CS, and Synthetic 6x
LinesPlant height (cm)Leaf ageRoot numberLeaf area (cm2 per plant)SP (mg/g FW)Chla (mg/g FW)Chlb (mg/g FW)Chla+Chlb (mg/g FW)Caro (mg/g FW)Fv/FmPn (μmol/(m2·s)
Synthetic 6x26.1b3.2b3.7c15.1c58.90a1.39a0.37a1.76a0.27a0.855a16.12b
A-D Ave32.70a3.61a3.95b19.68b47.23c1.33a0.36ab1.69a0.27a0.856 a17.18b
A-D Se2.300.140.742.316.
A-DCV (%)7.035.4518.7211.7612.747.829.987.907.350.96612.82
A Ave32.50a3.61a3.89bc19.31b43.31d1.33a0.37a1.70a0.27a0.856a17.86b
A Se1.620.130.521.386.
A CV (%)5.005.1513.337.1714.046.417.826.068.640.84911.50
B Ave32.99a3.59a4.00b19.96b49.02b1.34a0.37a1.72a0.27a0.853a17.48b
B Se2.400.121.082.314.
B CV (%)7.284.7026.8911.588.687.644.846.775.901.29114.87
D Ave32.61a3.64a3.97b19.77b49.37b1.31a0.34b1.65b0.26a0.860a16.21b
D Se3.010.180.633.
D CV (%)9.226.8615.7716.2712.5910.0714.4110.907.520.59611.46
Tab.2  Plant morphological traits and photosynthetic parameters in CSLs, CS, and Synthetic 6x
Independent variable (x)Accumulative N per plant (y)Accumulative P per plant (y)Accumulative K per plant (y)
y = ax + bry = ax + bry =ax + br
Plant heighty = 0.123x - 0.3420.501*y = -0.011x + 0.762-0.463y = 0.016x - 0.03940.425
Leaf agey = 0.774x + 1.8170.188y = 0.0778x + 0.43150.071y = 0.0638x + 0.31680.105
Primary root numbery = 0.375x + 2.3420.488*y = -0.011x + 0.762-0.463y = 0.066x + 0.2200.582
Leaf area per planty = 0.162x + 0.6380.661*y = 0.030x + 0.0360.457y = 0.022x + 0.0580.597*
Soluble protein contenty = 0.021x + 2.8680.211y = 0.003x + 0.5130.097y = 0.002x + 0.3910.137
Chlay = 3.099x - 0.2760.549*y = 0.261x + 0.2880.171y = 0.311x + 0.0710.374
Chlby = 6.524x + 1.4760.411y = 0.952x + 0.2900.222y = 0.884x + 0.1630.379
Chla+by = 2.352x - 0.1350.538*y = 0.229x + 0.2490.193y = 0.254x + 0.0550.394
Caroy = 6.796x + 1.9950.235y = -0.556x + 0.7860.071y = 0.265x + 0.4120.062
Fv/Fmy = 26.482x - 18.8340.391y = 2.176x - 1.2280.119y = 3.390x - 2.4190.340
Pny = 0.141x + 1.40110.564*y = 0.019x + 0.3120.476*y = 0.015x + 0.2260.485*
Tab.3  Regression analysis of accumulative N, P, and K, plant morphological traits and photosynthetic parameters in CSLs
1 Atienza S G, Ramírez C M, Hernández P, Martin A (2004). Chromosomal location of genes for carotenoid pigments in Hordeum chilense. Plant Breed , 123(3): 303-304
doi: 10.1111/j.1439-0523.2004.00918.x
2 Bai Z Y, Li C D, Feng L X, Sun H C (2007). Chromosomal localization of genes associated with spikelet differentiation and drought tolerance in Chinese Spring (recipient)/Synthetic 6x (donor). Scientica Agricultua Sinaca , 40(10): 2136-2144 (in Chinese)
3 Clua A A, Castro A M, Gimenez D O, Tacaliti M S, Worland A J (2002). Chromosomal effects in the endogenous contents of non-structural carbohydrates and proteins measured in wheat substitution lines. Plant Breed , 121(2): 141-145
doi: 10.1046/j.1439-0523.2002.00692.x
4 Delauney A J, Verma D P S (1993). Proline biosynthesis and osmoregulation in plants. Plant J , 4(2): 215-223
doi: 10.1046/j.1365-313X.1993.04020215.x
5 Foulkes M J, Sylvester-Bradley R, Scott R K (1998). Evidence for differences between wheat cultivars in acquisition of soil mineral nitrogen. J Agric Sci , 130: 29-44
doi: 10.1017/S0021859697005029
6 Galiba G, Kocsy G, Kaur-sawhney R, Sutka J, Galston A W (1993). Chromosomal localization of osmotic and salt stress-induced differential alterations in polyamine content in wheat. Plant Sci , 92(2): 203-211
doi: 10.1016/0168-9452(93)90207-G
7 Galiba G, Simon-Sarkadi L, Kocsy G, Salgo A, Sutka J (1992). Possible chromosomal location of genes determining the osmoregulation of wheat. Theor Appl Genet , 85(4): 415-418
doi: 10.1007/BF00222322
8 Glass A D M, Siddiqi M Y, Giles K I (1981). Correlations between potassium uptake and hydrogen efflux in barley varieties. Plant Physiol , 68: 457-459
9 Guo L, Long S X, Zhao F H, Bao J X, Guo C J, Xiao K (2008). Comparison and evaluation of biochemical criteria for phosphorus efficiency in wheat. Journal of Plant Genetic Resources , 9(4): 506-510 (in Chinese)
10 Han Y L, Liu X H, Wang Y L, Tan J F (2006). Potassium nutrition characteristics of different wheat varieties. Journal of Triticeae Crops , 26(1): 99-103 (in Chinese)
11 Karrou A, Maranville J W (1994). Response of wheat cultivars to different soil nitrogen and moisture regimes: II. Nitrogen uptake, partitioning and influx. J Plant Nutr , 17(5): 745-761
doi: 10.1080/01904169409364764
12 Law C N, Wang J (1997). Study on inter-varietal chromosome substitutions lines of wheat (Triticum aestivum L.). Journal of Triticeae Crops , 17(2): 20-24 (in Chinese)
13 Li S W, Wen H D, Zhou Y Z, Li Y H, Xiao K (2006). Characterization of nitrogen uptake and dry matter production in wheat varieties with different N efficiency. Scientia Agricultura Sinica , 39(10): 1992-2000 (in Chinese)
14 Liu B H, Wang H S, Yang L (1998). Study and realization on chromosome substitutions lines of wheat (Triticum aestivum L.). Bulletin of Biology , 33(4): 26-27 (in Chinese)
15 Morgan J M (1991). A gene controlling differences in osmoregulation in wheat. Aust J Plant Physiol , 18(3): 249-257
doi: 10.1071/PP9910249
16 Muurinen S, Slafer G A, Peltonen-Sainio P (2006). Breeding effects on nitrogen use efficiency of spring cereals under northern conditions. Crop Sci , 46(2): 561-568
doi: 10.2135/cropsci2005-05-0046
17 Read S M, Northcote D H (1981). Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for proteins. Anal Biochem , 116(1): 53-64
doi: 10.1016/0003-2697(81)90321-3 pmid:7304986
18 Sivamani E, Bahieldin A, Wraith J M, Al-Niemi T, Dyer W E, Ho T H D, Qu R (2000). Improved biomass productivity and water use efficiency under water deficit conditions in transgenic wheat constitutively expressing the barley HVA1 gene. Plant Sci , 155(1): 1-9
19 Sun C F, Dai T B, Jing Q, Jiang D, Cao W X (2004). Nitrogen use efficiency and its relationship with nitrogen nutrition characteristics of wheat varieties. Chinese Journal of Applied Ecology , 15(6): 983-987 (in Chinese)
20 Sun Q X, Quick J S (1991). Chromosomal locations of genes for heat tolerance in tetraploid wheat. Cereal Res Commun , 19(4): 431-437
21 Trehan S P (2005). Nutrient management by exploiting genetic diversity of potato—A review. Potato Journal , 32(1-2): 1-15
22 Trehan S P (2009). Improving nutrient use efficiency by exploiting genetic diversity of potato. Potato Journal , 36(3-4): 121-135
23 Wang Q R, Li J Y, Li Z S (1999). Studies on the critical values of phosphorus in wheat genotypes with phosphorus efficiencies. Acta Bot Boreal-Occident Sin , 19(3): 363-370
24 Yang K, Chang X P, Hu R H, Jia J Z (2001a). Chromosomal positioning of the genes of water use efficiency and concerned physiological traits in wheat leaves. Acta Agron Sin , 27(3): 363-366 (in Chinese)
25 Yang K, Chang X P, Hu R H, Jia J Z (2001b). Chromosomal localization of genes associated with proline accumulation under drought stress in wheat (Triticum aestivum L.). Acta Agron Sin , 27(3): 363-366 (in Chinese)
26 Zhang J, Zhang Z B, Xie H M, Dong B D, Hu M Y, Xu P (2005). Chromosomal positioning of the genes of water use efficiency and concerned physiological traits in wheat leaves. Acta Bot Boreal-Occident Sin , 25(8): 1521-1527 (in Chinese)
27 Zhang Y, Wang D S, Zhang Y, He Z H (2007). Variation of major mineral elements concentration and their relationships in grains of Chinese wheat. Scientia Agricultura Sinica , 40(9): 1871-1876 (in Chinese)
[1] Wenjing LU, Jincai LI, Fangpeng LIU, Juntao GU, Chengjin GUO, Liu XU, Huiyan ZHANG, Kai XIAO. Expression pattern of wheat miRNAs under salinity stress and prediction of salt-inducible miRNAs targets[J]. Front Agric Chin, 2011, 5(4): 413-422.
[2] Xirong CUI, Yongsheng ZHANG, Fanghua ZHAO, Chengjin GUO, Juntao GU, Wenjing LU, Xiaojuan LI, Kai XIAO. Molecular characterization and expression analysis of phosphate transporter gene TaPT2-1 in wheat (Triticum aestivum L.)[J]. Front Agric Chin, 2011, 5(3): 274-283.
Full text